LOCUS       40924_28886        9186 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pLV.DsRed, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Goncalves MA, de Vries AA, Holkers M, van de Watering MJ, van der 
            Velde I, van Nierop GP, Valerio D, Knaan-Shanzer S.
  TITLE     Human mesenchymal stem cells ectopically expressing full-length 
            dystrophin can complement Duchenne muscular dystrophy myotubes by 
            cell fusion
  JOURNAL   Hum. Mol. Genet. 15 (2), 213-221 (2006)
  PUBMED    16321987
REFERENCE   2  (bases 1 to 9186)
  AUTHORS   Goncalves MA, Swildens J, Holkers M, Narain A, van Nierop GP, van de
            Watering MJ, Knaan-Shanzer S, de Vries AA.
  TITLE     Genetic complementation of human muscle cells via directed stem cell
            fusion
  JOURNAL   Mol. Ther. 16 (4), 741-748 (2008)
  PUBMED    18334989
REFERENCE   3  (bases 1 to 9186)
  AUTHORS   Goncalves MA, de Vries AA.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUL-2007) Molecular Cell Biology, Leiden University 
            Medical Center, Einthovenweg 20, Leiden 2333 ZC, The Netherlands
REFERENCE   4  (bases 1 to 9186)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 9186)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum. Mol. 
            Genet."; date: "2006"; volume: "15"; issue: "2"; pages: "213-221"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Mol. 
            Ther."; date: "2008"; volume: "16"; issue: "4"; pages: "741-748"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (23-JUL-2007) Molecular Cell Biology, Leiden University Medical 
            Center, Einthovenweg 20, Leiden 2333 ZC, The Netherlands"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..808
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1459..1692
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1789..1906
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        3196..4377
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     LTR             4485..4753
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(4790..4808)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4900..5574
                     /codon_start=1
                     /label=DsRed-Express
                     /note="rapidly maturing tetrameric variant of DsRed
                     fluorescent protein (Bevis and Glick, 2002)"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEPSTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     repeat_region   5625..5859
                     /label=HIV-1 3' SIN LTR
                     /note="HIV-1 3' SIN LTR"
     LTR             5626..5858
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5883..5901)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5922..5938)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5946..5962)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5970..6000)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6015..6036)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6324..6912)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7304..7960)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(7961..8063)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     promoter        complement(8383..8487)
                     /label=AmpR promoter
     rep_origin      complement(8513..8968)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     9110..9126
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9136..9154
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tggaagggct aattcactcc caacaaagac aagatatcct tgatctgtgg gtctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggact agatggccac
      121 tgacctttgg atggtgcttc aagctagtac cagttgagcc agagaagata gaagaggcca
      181 atgcaggaga gaacaactgc ttgttacacc ctatgagcca gcatggaatg gatgacccgg
      241 agagagaagg gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtactac aagaactgat gacctcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggaag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tgctgcatat aagcagctgc ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta gctagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttt aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaaggaaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggcgagta cgccaaaaaa tttttgacta gcggaggcta
      781 gaaggagaga gtagggtgcg agagcgtcag tattaagtgc gggggaatta gataagtggg
      841 aaaaaattcg gttaaggcca gggggaaaga aacaatatag attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagttg atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccgt cccttcagac aggatcagaa gagcttagat
     1021 cattatataa tacagtagcc accctctatt gtgtacatca aaagatagag gtaaaagaca
     1081 ccaaggaagc tttagagaag atagaggaag agcaaaacaa aagtaagaaa aaagcacagc
     1141 aagcagcagc tgacacagga aacagcagcc aggtcagcca aaattaccct atagtgcaga
     1201 acctacaggg gcaaatggta catcaggcca tatcacctag aactttaaat gcatgggtaa
     1261 aagtagtgga agagaaggcg ttcagcccag aagtaatacc ctagttttca gcattatcag
     1321 aaggagccac cccacaagat tctagaattg gagaagtgaa ttatataaat ataaagtagt
     1381 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     1441 aaaaagagca gtgggactag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     1501 tatgggcgca gcgtcaataa cgctgacggt acaggccaga caattattgt ctggtatagt
     1561 gcaacagcag aacaatctgc tgagggctat tgaggcgcaa cagcacctgt tgcaactcac
     1621 agtctggggc atcaagcagc tccaggcaag agtcctggct gtggaaagat acctaaggga
     1681 tcaacagctc ctagggattt ggggttgctc tggaaaactc atttgcacca ctactgtgcc
     1741 ttggaatact agttggagta ataaatctct gaatgaattc atccacaatt ttaaaagaaa
     1801 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     1861 acaaactaaa gaactacaga aacaaattac aaaaattcaa aattttcggg tttattacag
     1921 ggacaggatc agcttcttct agaggtaccg catgcgatct agcttgcatg cctgcaggtc
     1981 acttagcgtt ggccacatag taggttctca aatacttgtt aataaataag tttgttcgag
     2041 aagctgggca atgatattct acagctggaa gaagaaacat aatgatctag taattagctc
     2101 aattaaaaat aaacgttctt ctttcctcag aggagcattt cccaaggcct gccttgatag
     2161 ccatccaaaa aggccaagct catccaatct tgccctagat ttatgctaaa atgcagttac
     2221 aatcgatagg atgacagaaa acgacagcac ttatttaaat ataataggca cttatttaaa
     2281 taggagaagc tgtgacttca tagcaagtgt tggggttagg aaactgggtg gataaacttg
     2341 ctgatgctgt agatcttagc ctctacatga gatcatgtgg aaaatctgaa agcattttag
     2401 gttccttatg tttgcaatca aataactgta caccttttaa tttaaaaagt accatgaggc
     2461 acacacacac actcgcagga actttttggc gtaacaaaac tagaattaga tctaaaagct
     2521 aactgtagga ctgagtctat tctaaactga aagcctggac atctggagta ccagggggag
     2581 atgacgtgtt acgggcttcc ataaaagcag ctggctttga atggaaggag ccaagaggcc
     2641 agcacaggag cggattcgtc gctttcacgg ccatcgagcc gaacctctcg caagtccgtg
     2701 agccgttaag gaggccccca gtcccgaccc ttcgccccaa gcccctcggg gtccccgggc
     2761 ctggtactcc ttgccacacg ggaggggcgc ggaagccggg gcggaggagg agccaacccc
     2821 gggctgggct gagacccgca gaggaagacg ctctagggat ttgtcccgga ctagcgagat
     2881 ggcaaggctg aggacgggag gctgattgag aggcgaaggt acaccctaat ctcaatacaa
     2941 cctttggagc taagccagca atggtagagg gaagattctg cacgtccctt ccaggcggcc
     3001 tccccgtcac cacccccccc aacccgcccc gaccggagct gagagtaatt catacaaaag
     3061 gactcgcccc tgccttgggg aatcccaggg accgtcgtta aactcccact aacgtagaac
     3121 ccagagatcg ctgcgttccc gccccctcac ccgcccgctc tcgtcatcac tgaggtggag
     3181 aagagcatgc gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     3241 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg
     3301 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     3361 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     3421 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt
     3481 gcgtgccttg aattacttcc acgcccctgg ctgcagtacg tgattcttga tcccgagctt
     3541 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     3601 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     3661 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     3721 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     3781 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     3841 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     3901 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     3961 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     4021 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     4081 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     4141 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     4201 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     4261 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     4321 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     4381 attatcgagc tctcccggga attcgatcgg cctctgagct attccagaag tagtgaggag
     4441 gcttttttgg aggcctaggc ttttgcaaaa agctcctcga tcgaggggct cgcatctctc
     4501 cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc
     4561 cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca
     4621 ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct
     4681 ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc
     4741 gccgttacag atccaagctc ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag
     4801 tcgtattacg cgcgatcgag ctctcccaat actagatcgc atgcctgcag agctctagag
     4861 tcgacggtac cgcgggcccg ggatccacta gtcgccacca tggcctcctc cgaggacgtc
     4921 atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc
     4981 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     5041 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttccagtac
     5101 ggctccaagg tgtacgtgaa gcaccccgcc gacatccccg actacaagaa gctgtccttc
     5161 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     5221 acccaggact cctccctgca ggacggctgc ttcatctaca aggtgaagtt catcggcgtg
     5281 aacttcccct ccgacggccc cgtaatgcag aagaagacta tgggctggga gccctccacc
     5341 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccacaaggc cctgaagctg
     5401 aaggacggcg gccactacct ggtggagttc aagtccatct acatggccaa gaagcccgtg
     5461 cagctgcccg gctactacta cgtggactcc aagctggaca tcacctccca caacgaggac
     5521 tacaccatcg tggagcagta cgagcgcgcc gagggccgcc accacctgtt cctgtagcgg
     5581 cctatgctat ggatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     5641 actcccaaca aagacaagat ctgcttttgc ctgtactggg tctctctggt tagaccagat
     5701 ctgagcctgg gagctctctg gctagctagg aaacccactg cttaagcctc aataaagctt
     5761 gccttgagtg ctttaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5821 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccggtacc cagcttttgt
     5881 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     5941 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6001 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6061 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6121 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6181 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6241 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6301 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6361 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6421 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6481 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6541 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6601 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6661 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6721 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     6781 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6841 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6901 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6961 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7021 tttgatcaga aaaaaaggat catatcgtca attattacct ccacggggag agcctgagca
     7081 aactggcctc aggcatttga gaagcacacg gtcacactgc ttccggtagt caataaaccg
     7141 gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc gacgaccggg
     7201 tcgaatttgc tttcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc
     7261 aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact
     7321 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     7381 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     7441 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     7501 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     7561 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     7621 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     7681 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     7741 gtaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     7801 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     7861 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     7921 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     7981 aaaatctcga caactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     8041 tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc
     8101 cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta
     8161 tttattcggt cgaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8221 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8281 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8341 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8401 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8461 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     8521 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     8581 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     8641 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     8701 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     8761 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     8821 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     8881 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     8941 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     9001 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     9061 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     9121 gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca ccgcggtggc
     9181 ggccgc
//
