LOCUS       V017085                14994 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017085
VERSION     V017085
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14994)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label="UbC promoter"
                     /note="human ubiquitin C promoter"
     CDS             1292..1315
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             1484..1504
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             1514..5617
                     /label="dCas9"
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
     CDS             5882..6595
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    6669..7257
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7782..7962
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7994..8218
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8264..8692
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        8706..9035
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        9083..9130
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             9149..9520
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    9653..9786
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(9871..9901)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9916..9937)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(10225..10813)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10987..11844)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(11845..11949)
                     /label="AmpR promoter"
     enhancer        12215..12594
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12595..12797
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             12812..12992
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    13039..13164
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    13657..13890
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             14075..14119
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             14268..14309
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    14417..14534
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     protein_bind    complement(14578..14611)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_RNA        complement(14622..14697)
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     promoter        complement(14722..14962)
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg ggctgcaggt cgactctaga gccaccatgg actacaaaga
     1261 ccatgacggt gattataaag atcatgacat cgattacaag gatgacgatg acaagcacgg
     1321 tccgcgggct gacgcattgg acgattttga tctggatatg ctgggaagtg acgccctcga
     1381 tgattttgac cttgacatgc ttggttcgga tgcccttgat gactttgacc tcgacatgct
     1441 cggcagtgac gcccttgatg atttcgacct ggacatggtt aaccccaaga agaagaggaa
     1501 ggtgggccgc ggaatggaca agaagtactc cattgggctc gccatcggca caaacagcgt
     1561 cggctgggcc gtcattacgg acgagtacaa ggtgccgagc aaaaaattca aagttctggg
     1621 caataccgat cgccacagca taaagaagaa cctcattggc gccctcctgt tcgactccgg
     1681 ggaaaccgcc gaagccacgc ggctcaaaag aacagcacgg cgcagatata cccgcagaaa
     1741 gaatcggatc tgctacctgc aggagatctt tagtaatgag atggctaagg tggatgactc
     1801 tttcttccat aggctggagg agtccttttt ggtggaggag gataaaaagc acgagcgcca
     1861 cccaatcttt ggcaatatcg tggacgaggt ggcgtaccat gaaaagtacc caaccatata
     1921 tcatctgagg aagaagcttg tagacagtac tgataaggct gacttgcggt tgatctatct
     1981 cgcgctggcg catatgatca aatttcgggg acacttcctc atcgaggggg acctgaaccc
     2041 agacaacagc gatgtcgaca aactctttat ccaactggtt cagacttaca atcagctttt
     2101 cgaagagaac ccgatcaacg catccggagt tgacgccaaa gcaatcctga gcgctaggct
     2161 gtccaaatcc cggcggctcg aaaacctcat cgcacagctc cctggggaga agaagaacgg
     2221 cctgtttggt aatcttatcg ccctgtcact cgggctgacc cccaacttta aatctaactt
     2281 cgacctggcc gaagatgcca agcttcaact gagcaaagac acctacgatg atgatctcga
     2341 caatctgctg gcccagatcg gcgaccagta cgcagacctt tttttggcgg caaagaacct
     2401 gtcagacgcc attctgctga gtgatattct gcgagtgaac acggagatca ccaaagctcc
     2461 gctgagcgct agtatgatca agcgctatga tgagcaccac caagacttga ctttgctgaa
     2521 ggcccttgtc agacagcaac tgcctgagaa gtacaaggaa attttcttcg atcagtctaa
     2581 aaatggctac gccggataca ttgacggcgg agcaagccag gaggaatttt acaaatttat
     2641 taagcccatc ttggaaaaaa tggacggcac cgaggagctg ctggtaaagc ttaacagaga
     2701 agatctgttg cgcaaacagc gcactttcga caatggaagc atcccccacc agattcacct
     2761 gggcgaactg cacgctatcc tcaggcggca agaggatttc tacccctttt tgaaagataa
     2821 cagggaaaag attgagaaaa tcctcacatt tcggataccc tactatgtag gccccctcgc
     2881 ccggggaaat tccagattcg cgtggatgac tcgcaaatca gaagagacca tcactccctg
     2941 gaacttcgag gaagtcgtgg ataagggggc ctctgcccag tccttcatcg aaaggatgac
     3001 taactttgat aaaaatctgc ctaacgaaaa ggtgcttcct aaacactctc tgctgtacga
     3061 gtacttcaca gtttataacg agctcaccaa ggtcaaatac gtcacagaag ggatgagaaa
     3121 gccagcattc ctgtctggag agcagaagaa agctatcgtg gacctcctct tcaagacgaa
     3181 ccggaaagtt accgtgaaac agctcaaaga agactatttc aaaaagattg aatgtttcga
     3241 ctctgttgaa atcagcggag tggaggatcg cttcaacgca tccctgggaa cgtatcacga
     3301 tctcctgaaa atcattaaag acaaggactt cctggacaat gaggagaacg aggacattct
     3361 tgaggacatt gtcctcaccc ttacgttgtt tgaagatagg gagatgattg aagaacgctt
     3421 gaaaacttac gctcatctct tcgacgacaa agtcatgaaa cagctcaaga ggcgccgata
     3481 tacaggatgg gggcggctgt caagaaaact gatcaatggg atccgagaca agcagagtgg
     3541 aaagacaatc ctggattttc ttaagtccga tggatttgcc aaccggaact tcatgcagtt
     3601 gatccatgat gactctctca cctttaagga ggacatccag aaagcacaag tttctggcca
     3661 gggggacagt cttcacgagc acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg
     3721 aatactgcag accgttaagg tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc
     3781 cgagaatatc gttatcgaga tggcccgaga gaaccaaact acccagaagg gacagaagaa
     3841 cagtagggaa aggatgaaga ggattgaaga gggtataaaa gaactggggt cccaaatcct
     3901 taaggaacac ccagttgaaa acacccagct tcagaatgag aagctctacc tgtactacct
     3961 gcagaacggc agggacatgt acgtggatca ggaactggac atcaatcggc tctccgacta
     4021 cgacgtggat gccatcgtgc cccagtcttt tctcaaagat gattctattg ataataaagt
     4081 gttgacaaga tccgataaaa atagagggaa gagtgataac gtcccctcag aagaagttgt
     4141 caagaaaatg aaaaattatt ggcggcagct gctgaacgcc aaactgatca cacaacggaa
     4201 gttcgataat ctgactaagg ctgaacgagg tggcctgtct gagttggata aagccggctt
     4261 catcaaaagg cagcttgttg agacacgcca gatcaccaag cacgtggccc aaattctcga
     4321 ttcacgcatg aacaccaagt acgatgaaaa tgacaaactg attcgagagg tgaaagttat
     4381 tactctgaag tctaagctgg tctcagattt cagaaaggac tttcagtttt ataaggtgag
     4441 agagatcaac aattaccacc atgcgcatga tgcctacctg aatgcagtgg taggcactgc
     4501 acttatcaaa aaatatccca agcttgaatc tgaatttgtt tacggagact ataaagtgta
     4561 cgatgttagg aaaatgatcg caaagtctga gcaggaaata ggcaaggcca ccgctaagta
     4621 cttcttttac agcaatatta tgaatttttt caagaccgag attacactgg ccaatggaga
     4681 gattcggaag cgaccactta tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa
     4741 gggtagggat ttcgcgacag tccggaaggt cctgtccatg ccgcaggtga acatcgttaa
     4801 aaagaccgaa gtacagaccg gaggcttctc caaggaaagt atcctcccga aaaggaacag
     4861 cgacaagctg atcgcacgca aaaaagattg ggaccccaag aaatacggcg gattcgattc
     4921 tcctacagtc gcttacagtg tactggttgt ggccaaagtg gagaaaggga agtctaaaaa
     4981 actcaaaagc gtcaaggaac tgctgggcat cacaatcatg gagcgatcaa gcttcgaaaa
     5041 aaaccccatc gactttctcg aggcgaaagg atataaagag gtcaaaaaag acctcatcat
     5101 taagcttccc aagtactctc tctttgagct tgaaaacggc cggaaacgaa tgctcgctag
     5161 tgcgggcgag ctgcagaaag gtaacgagct ggcactgccc tctaaatacg ttaatttctt
     5221 gtatctggcc agccactatg aaaagctcaa agggtctccc gaagataatg agcagaagca
     5281 gctgttcgtg gaacaacaca aacactacct tgatgagatc atcgagcaaa taagcgaatt
     5341 ctccaaaaga gtgatcctcg ccgacgctaa cctcgataag gtgctttctg cttacaataa
     5401 gcacagggat aagcccatca gggagcaggc agaaaacatt atccacttgt ttactctgac
     5461 caacttgggc gcgcctgcag ccttcaagta cttcgacacc accatagaca gaaagcggta
     5521 cacctctaca aaggaggtcc tggacgccac actgattcat cagtcaatta cggggctcta
     5581 tgaaacaaga atcgacctct ctcagctcgg tggagacagc agggctgacc ccaagaagaa
     5641 gaggaaggtg gctagccgcg ccgacgcgct ggacgatttc gatctcgaca tgctgggttc
     5701 tgatgccctc gatgactttg acctggatat gttgggaagc gacgcattgg atgactttga
     5761 tctggacatg ctcggctccg atgctctgga cgatttcgat ctcgatatgt taatcgctag
     5821 cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctggtac
     5881 cgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     5941 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     6001 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     6061 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     6121 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     6181 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     6241 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     6301 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     6361 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     6421 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     6481 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     6541 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaccgg
     6601 ttgataatag ataacttcgt atagcataca ttatacgaag ttatgaattc gatatcaagc
     6661 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6721 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6781 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6841 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6901 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6961 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     7021 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     7081 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     7141 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     7201 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     7261 gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     7321 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     7381 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7441 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     7501 ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag
     7561 ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     7621 aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc
     7681 tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag
     7741 catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
     7801 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7861 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7921 gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc
     7981 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     8041 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     8101 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     8161 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     8221 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     8281 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     8341 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     8401 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     8461 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     8521 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     8581 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     8641 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     8701 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     8761 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     8821 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     8881 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8941 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     9001 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     9061 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
     9121 ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct
     9181 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
     9241 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
     9301 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
     9361 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
     9421 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
     9481 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
     9541 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     9601 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     9661 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     9721 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     9781 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     9841 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     9901 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     9961 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    10021 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    10081 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    10141 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    10201 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    10261 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    10321 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    10381 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    10441 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    10501 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    10561 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    10621 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    10681 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    10741 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    10801 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    10861 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    10921 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    10981 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    11041 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    11101 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    11161 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    11221 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    11281 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    11341 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    11401 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    11461 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    11521 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    11581 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    11641 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    11701 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    11761 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    11821 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    11881 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    11941 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
    12001 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
    12061 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
    12121 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
    12181 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
    12241 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
    12301 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
    12361 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
    12421 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
    12481 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
    12541 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
    12601 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
    12661 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
    12721 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
    12781 gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc
    12841 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
    12901 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    12961 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
    13021 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
    13081 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
    13141 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
    13201 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
    13261 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
    13321 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
    13381 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
    13441 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
    13501 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
    13561 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
    13621 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
    13681 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
    13741 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
    13801 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
    13861 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
    13921 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
    13981 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
    14041 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
    14101 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
    14161 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
    14221 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
    14281 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
    14341 cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg
    14401 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
    14461 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
    14521 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaata
    14581 acttcgtata gcatacatta tacgaagtta ttaaaaaaaa agcaccgact cggtgccact
    14641 ttttcaagtt gataacggac tagccttatt ttaacttgct atttctagct ctaaaactga
    14701 gacgtccgtc tccggtgttt cgtcctttcc acaagatata taaagccaag aaatcgaaat
    14761 actttcaagt tacggtaagc atatgatagt ccattttaaa acataatttt aaaactgcaa
    14821 actacccaag aaattattac tttctacgtc acgtattttg tactaatatc tttgtgttta
    14881 cagtcaaatt aattccaatt atctctctaa cagccttgta tcgtatatgc aaatatgaag
    14941 gaatcatggg aaataggccc tcttaattaa ttaacccgtg tcggctccag atct
//
