LOCUS       V033180                13221 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033180
VERSION     V033180
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2677
                     /label="ZBTB48(NM_005341)"
                     /note="ZBTB48(NM_005341)"
                     /gene="ZBTB48"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2750..3091
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3217..3716
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3737..4444
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4445..4501
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4502..5101
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5112..5700
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5763..5996
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6021..6039)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6828..6932
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6933..7793
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7964..8552
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8798..9127
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10261..10326
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10456..10476
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10901..11035
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11204..11837
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11884..12009
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12501..12734
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12919..12963
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctatccgg ttcgcagtga
      601 actccaagcc accatggacg gctccttcgt ccagcacagt gtgagggttc tgcaggagct
      661 caacaagcag cgggagaagg gccagtactg cgacgccact ctggacgtgg ggggcctggt
      721 gtttaaggca cactggagtg tccttgcctg ctgcagtcac tttttccaga gcctctacgg
      781 ggatggctca gggggcagtg tcgtcctccc tgctggcttc gctgagatct ttggcctctt
      841 gttggacttt ttctacactg gtcacctcgc tctcacctca gggaaccggg atcaggtgct
      901 cctggcagcc agggagttgc gagtgccaga ggccgtagag ctgtgccaga gcttcaagcc
      961 caaaacttca gtgggacagg cagcaggtgg ccagagtggg ctggggcccc ctgcctccca
     1021 gaatgtgaac agccacgtca aggagccggc aggcttggaa gaagaggaag tttcgaggac
     1081 tctgggtcta gtccccaggg atcaggagcc cagaggcagt catagtcctc agaggcccca
     1141 gctccattcc ccagctcaga gtgagggccc ctcctccctc tgtgggaaac tgaagcaggc
     1201 cttgaagcct tgtccccttg aggacaagaa acccgaggac tgcaaagtgc ccccaaggcc
     1261 cttagaggct gaaggtgccc agctgcaggg cggcagtaat gagtgggaag tggtggttca
     1321 agtggaggat gatggggatg gcgattacat gtctgagcct gaggctgtgc tgaccaggag
     1381 gaagtcaaat gtaatccgaa agccctgtgc agctgagcca gccctgagcg cgggctccct
     1441 agcagctgag cctgctgaga acagaaaagg tacagcggtg ccggtcgaat gccccacatg
     1501 tcataaaaag ttcctcagca aatattatct aaaagtccac aacaggaaac atactgggga
     1561 gaaacccttt gagtgtccca aatgtgggaa gtgttacttt cggaaggaga acctcctgga
     1621 gcatgaagcc cggaattgca tgaaccgctc ggaacaggtc ttcacgtgct ctgtgtgcca
     1681 ggagacattc cgccgaagga tggagctgcg ggtgcacatg gtgtctcaca caggggagat
     1741 gccctacaag tgttcctcct gctcccagca gttcatgcag aagaaggact tgcagagcca
     1801 catgatcaaa cttcatggag cccccaagcc ccatgcatgc cccacctgtg ccaagtgctt
     1861 cctgtctcgg acagagctgc agctgcatga agctttcaag caccgtggtg agaagctgtt
     1921 tgtgtgtgag gagtgtgggc accgggcctc gagccggaat ggcctgcaga tgcacatcaa
     1981 ggccaagcac aggaatgaga ggccacacgt atgtgagttc tgcagccacg ccttcaccca
     2041 aaaggccaat ctcaacatgc acctgcgcac acacacgggt gagaagccct tccagtgcca
     2101 cctctgtggc aagaccttcc gaacccaagc cagcctggac aagcacaacc gcacccacac
     2161 cggggaaagg cccttcagtt gcgagttctg tgaacagcgc ttcactgaga aggggcccct
     2221 cctgaggcac gtggccagcc gccatcagga gggccggccc cacttctgcc agatatgcgg
     2281 caagaccttc aaagccgtgg agcaactgcg tgtgcacgtc agacggcaca agggggtgag
     2341 gaagtttgag tgcaccgagt gtggctacaa gtttacccga caggcccacc tgcggaggca
     2401 catggagatc cacgaccggg tagagaacta caacccgcgg cagcgcaagc tccgcaacct
     2461 gatcatcgag gacgagaaga tggtggtggt ggcgctgcag ccgcctgcag agctggaggt
     2521 gggctcggcg gaggtcattg tggagtccct ggcccagggc ggcctggcct cccagctccc
     2581 cggccagaga ctgtgtgcag aggagagctt caccggccca ggtgtcctgg agccctccct
     2641 catcatcaca gctgctgtcc ccgaggactg tgacacatac ccctacgacg tgcctgacta
     2701 cgccggcggc ggtggttcag gcggtggcgg ttccggtggt gggggatcag tccaactggt
     2761 ggagtctggt ggcgctttgg tgcagccagg tggctctctg cgtttgtcct gtgccgcttc
     2821 tggcttccca gtgaaccgct attccatgcg ctggtatcgc caggctccag gcaaagagcg
     2881 tgagtgggta gccggtatgt ccagcgcggg tgatcgtagc tcctatgaag actccgtgaa
     2941 gggccgtttc accatcagcc gtgacgatgc ccgtaacacg gtgtatctgc aaatgaacag
     3001 cttgaaacct gaagatacgg ccgtgtatta ctgtaatgtg aacgtgggct tcgagtattg
     3061 gggccaaggc acccaggtca ccgtctccag ctaagatcct tgacttgcgg ccgcaactcc
     3121 cacctgcaac atgcgtgact gactgaggcc gcgactctag agtcgacctg catctaggcg
     3181 ccggaattag atctctcgag gttaacgaat tctaccgggt aggggaggcg cttttcccaa
     3241 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
     3301 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
     3361 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
     3421 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
     3481 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
     3541 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
     3601 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
     3661 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
     3721 gcagcccaag cttaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     3781 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     3841 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     3901 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     3961 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     4021 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     4081 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     4141 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     4201 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     4261 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     4321 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     4381 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     4441 caaggcaaca aacttctctc tgctgaaaca agccggagat gtcgaagaga atcctggacc
     4501 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     4561 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     4621 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     4681 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     4741 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     4801 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     4861 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     4921 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     4981 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     5041 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     5101 atcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5161 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5221 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5281 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     5341 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     5401 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     5461 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     5521 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     5581 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     5641 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     5701 atcgataccg tcgaccaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5761 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     5821 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5881 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5941 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     6001 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
     6061 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
     6121 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
     6181 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
     6241 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     6301 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     6361 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     6421 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     6481 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     6541 cgtcgaatgg tgcactctca gtacaatcta gctctgatgc cgcatagtta agccagcccc
     6601 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     6661 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     6721 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     6781 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6841 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6901 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6961 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7021 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7081 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7141 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7201 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7261 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7321 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7381 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7441 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7501 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7561 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7621 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7681 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7741 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7801 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7861 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7921 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7981 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8041 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8101 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8161 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8221 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8281 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8341 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8401 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8461 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8521 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8581 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8641 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8701 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8761 ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa
     8821 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8881 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     8941 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     9001 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9061 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     9121 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc
     9181 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
     9241 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
     9301 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
     9361 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
     9421 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat
     9481 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
     9541 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
     9601 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
     9661 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
     9721 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
     9781 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
     9841 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
     9901 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
     9961 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc
    10021 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg
    10081 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg
    10141 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa
    10201 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
    10261 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
    10321 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
    10381 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
    10441 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
    10501 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
    10561 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
    10621 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
    10681 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
    10741 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
    10801 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
    10861 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
    10921 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    10981 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
    11041 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
    11101 caattaccta gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    11161 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac
    11221 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
    11281 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
    11341 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
    11401 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
    11461 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
    11521 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
    11581 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
    11641 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
    11701 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
    11761 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
    11821 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
    11881 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
    11941 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
    12001 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
    12061 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
    12121 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
    12181 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
    12241 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
    12301 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggtgatctt
    12361 cagacctgga cgatatatat gagggacaat tggagaagtg aattatataa atataaagta
    12421 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
    12481 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
    12541 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
    12601 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
    12661 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
    12721 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
    12781 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
    12841 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
    12901 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
    12961 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
    13021 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
    13081 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
    13141 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
    13201 gtgaacggat ctcgacggtc g
//
