LOCUS       V030216                14196 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030216
VERSION     V030216
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..3652
                     /label="ZBTB4(NM_020899)"
                     /note="ZBTB4(NM_020899)"
                     /gene="ZBTB4"
     CDS             3653..3679
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3725..4066
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        4192..4691
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4712..5419
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5420..5476
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             5477..6076
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6087..6675
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6738..6971
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6996..7014)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7803..7907
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7908..8768
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8939..9527
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9773..10102
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          11236..11301
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             11431..11451
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11876..12010
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             12179..12812
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12859..12984
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    13476..13709
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13894..13938
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttcttcattg gtagcagtga
      601 actccaagcc accatgcccc cccctgcaga ggtgacggac ccgtcccatg cccccgccgt
      661 cctgcgccag ctcaatgaac agcggctccg tggcctcttc tgtgacgtca ccctcatagc
      721 cggagacacc aagttccctg ctcaccgcag cgtcctggct gcttcaagtc ccttcttcag
      781 agaggccctg ctcacttcag ccccactacc ccttccacca gctactgggg gcgccgcacc
      841 caaccctgcc accaccacag ctgcctcttc ctcctcctcc tcttcctcgt cttcttcctc
      901 ttcttcctcc tctgcttctt cttcttcttc ctcttcctct tcctctcccc ctccagcctc
      961 tccccctgct tcttccccac cccgggtcct ggagttgcca ggagtcccag cagctgcgtt
     1021 ttctgatgtc ctcaacttca tctacagcgc ccggctcgca ctgcctggtg gtggagggga
     1081 cggggcagct gtagcagaga ttggagcttt ggggcgccgt ctgggcatct cccgccttca
     1141 gggcctgggg gagggaggtg atgcctgggt acctcctacc ccagccccca tggccacctc
     1201 gcagcctgaa gaggacagct ttgggcccgg gcccaggcca gctggggagt gggagggtga
     1261 cagggctgag gcccaggccc ctgacttgca gtgctccctg ccccggcggc ccctcccctg
     1321 cccccagtgt ggaaaaagct tcatccatcc caaacggctg cagacccatg aggcccagtg
     1381 ccgacgaggg gccagcacgc gggggtctac agggctggga gctgggggcg ctggccctgg
     1441 tggtcctgca ggggtggacg cctcagccct gcctccacca gtgggcttcc gagggggccc
     1501 cgagcacgtg gtgaaggtgg tgggcggcca cgtgctgtat gtgtgcgcgg cctgcgagcg
     1561 ttcctacgtg accctgtcca gtctgaagag acacagcaat gtacactcgt ggcggaggaa
     1621 gtacccctgc cgctattgtg agaaagtgtt tgctctggcg gagtaccgca cgaagcatga
     1681 agtgtggcac acgggggagc gcaggtacca gtgcatcttc tgttgggaga cctttgtcac
     1741 ttactataac ctgaagaccc accagcgagc cttccacggc attagcccgg gcctccttgc
     1801 cagtgagaag acacccaatg gaggctacaa gcccaagctc aatacactca agctctaccg
     1861 cctgctcccc atgcgggcag ccaagcggcc ctacaagacc tacagccagg gagccccgga
     1921 ggctcccctt tctccaaccc tcaacacacc ggcccctgtg gcaatgccag ccagcccgcc
     1981 gcctgggcct ccacctgccc cagagcctgg ccctccaccc tctgtcatca cttttgccca
     2041 cccagccccc tctgtcattg tccatggggg cagtagcagt ggtggagggg ggagtgggac
     2101 ggccagcaca ggagggtccc aagctgcctc ggttatcact tacactgctc ccccgaggcc
     2161 acccaagaaa cgagaatacc cacctcctcc ccctgagcct gcagccacac ccaccagccc
     2221 agccacagca gtcagcccag ccaccgctgc agggccagcc atggccacca ccacggagga
     2281 ggccaagggc cggaatccac gggctggaag gactctgact tacacagcca agccagtggg
     2341 cgggattggt ggaggtgggg gtccccccac aggggctggc cggggcccct ctcagctgca
     2401 ggctccacct ccactgtgtc agatcactgt gcgaataggg gaggaggcca tcgtcaagcg
     2461 ccgcatctca gagactgacc tgcgtcctgg ggagctgagc ggagaggaga tggaggagag
     2521 tgaggaggac gaagaggagg aggacgaaga ggaggaggag gaggatgagg aggaatcaaa
     2581 ggctggtggg gaggaccagc tctggaggcc ctactactcc tacaagccta agcgcaaggc
     2641 tggagctgct ggaggtgcca gtgtgggggg cagtgggctg ccccgaggcc gccggccacc
     2701 acgttggagg cagaagctgg aacggaggag ctgggaggaa accccagcgg ccgagagccc
     2761 agcgggacgt gcccgcacag agcggaggca ccgatgcggg gactgtgccc agaccttcac
     2821 caccctgaga aagctgcgga agcaccaaga ggcccacggt gggggctccc acagctcccg
     2881 ggccggacgg aggccctcca cccgctttac ctgcccccac tgcgccaagg tgtgcaagac
     2941 cgcagctgcc ctgagccgcc acgggcagag gcatgctgct gagcggcccg ggggcacccc
     3001 aacccctgtc attgcctatt ccaagggcag cgctggcacc aggcccgggg atgtcaagga
     3061 ggaagccccc caagagatgc aagtctcctc atccagcggt gaggcaggtg gcgggagcac
     3121 tgctgctgag gaagcttccg agaccgcctc actccaggac cctatcattt cagggggtga
     3181 ggagccccca gtagtggcaa gcgggggcag ctatgtatac ccacctgtgc aggaatttcc
     3241 actggccttg attgggggcg gccgggaacc tggcggtggc aggggaaaat ctgggagtga
     3301 agggccagtg ggggctggtg agggggaccg gatggagggg ataggggctg ccaaagtcac
     3361 tttctaccct gagccctacc cgctcgtcta tggcccccag ctccttgccg cctaccctta
     3421 caacttcagt aacttggccg ctctcccggt tgctctcaac atggtcctac ctgatgagaa
     3481 gggtgcgggg gcccttccct tcctaccagg ggtctttggc tacgcagtga atcctcaagc
     3541 agcaccccct gccccaccaa caccacctcc cccaactctt cctccaccaa ttccccctaa
     3601 gggagaaggg gaaagggcag gggttgagag aacccagaag ggcgatgtgg ggtaccccta
     3661 cgacgtgcct gactacgccg gcggcggtgg ttcaggcggt ggcggttccg gtggtggggg
     3721 atcagtccaa ctggtggagt ctggtggcgc tttggtgcag ccaggtggct ctctgcgttt
     3781 gtcctgtgcc gcttctggct tcccagtgaa ccgctattcc atgcgctggt atcgccaggc
     3841 tccaggcaaa gagcgtgagt gggtagccgg tatgtccagc gcgggtgatc gtagctccta
     3901 tgaagactcc gtgaagggcc gtttcaccat cagccgtgac gatgcccgta acacggtgta
     3961 tctgcaaatg aacagcttga aacctgaaga tacggccgtg tattactgta atgtgaacgt
     4021 gggcttcgag tattggggcc aaggcaccca ggtcaccgtc tccagctaag atccttgact
     4081 tgcggccgca actcccacct gcaacatgcg tgactgactg aggccgcgac tctagagtcg
     4141 acctgcatct aggcgccgga attagatctc tcgaggttaa cgaattctac cgggtagggg
     4201 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     4261 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     4321 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     4381 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     4441 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     4501 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     4561 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     4621 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     4681 cgggcctttc gacctgcagc ccaagcttac catggtgagc aagggcgagg aggataacat
     4741 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     4801 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     4861 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     4921 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     4981 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     5041 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     5101 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     5161 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     5221 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     5281 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     5341 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     5401 catggacgag ctgtacaagg caacaaactt ctctctgctg aaacaagccg gagatgtcga
     5461 agagaatcct ggaccgatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     5521 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     5581 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     5641 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     5701 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     5761 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     5821 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     5881 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     5941 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     6001 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     6061 caagcccggt gcctgatctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     6121 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6181 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6241 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6301 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6361 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6421 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6481 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6541 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6601 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     6661 gggccgcctc cccgcatcga taccgtcgac caaggcagct gtagatctta gccacttttt
     6721 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     6781 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6841 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6901 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6961 aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg
     7021 tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag
     7081 cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc
     7141 gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg
     7201 gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc
     7261 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     7321 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     7381 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     7441 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     7501 tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctagctct gatgccgcat
     7561 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     7621 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     7681 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     7741 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     7801 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7861 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7921 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7981 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8041 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8101 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8161 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8221 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8281 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8341 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8401 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8461 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8521 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8581 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8641 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8701 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8761 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8821 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8881 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8941 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9001 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9061 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     9121 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9181 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9241 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9301 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9361 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9421 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9481 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9541 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9601 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9661 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     9721 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca
     9781 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     9841 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     9901 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     9961 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    10021 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    10081 tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga
    10141 gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg
    10201 aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga
    10261 acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg
    10321 tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg
    10381 aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc
    10441 gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa
    10501 ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat
    10561 tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc
    10621 gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt
    10681 tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc
    10741 gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa
    10801 cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg
    10861 caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag
    10921 tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca
    10981 tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac
    11041 gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg
    11101 atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg
    11161 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
    11221 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
    11281 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
    11341 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
    11401 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
    11461 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
    11521 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
    11581 atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca taacatactg
    11641 ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg
    11701 tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc
    11761 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
    11821 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
    11881 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    11941 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    12001 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
    12061 ccctattacc actgccaatt acctagtggt ttcatttact ctaaacctgt gattcctctg
    12121 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg
    12181 gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca
    12241 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
    12301 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
    12361 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
    12421 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
    12481 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
    12541 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
    12601 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
    12661 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
    12721 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    12781 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
    12841 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
    12901 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
    12961 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
    13021 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
    13081 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
    13141 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
    13201 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
    13261 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
    13321 gcggccggtg atcttcagac ctggacgata tatatgaggg acaattggag aagtgaatta
    13381 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
    13441 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
    13501 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
    13561 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
    13621 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
    13681 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
    13741 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
    13801 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
    13861 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
    13921 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
    13981 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
    14041 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
    14101 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
    14161 agatccattc gattagtgaa cggatctcga cggtcg
//
