LOCUS       V033585                13380 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033585
VERSION     V033585
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2836
                     /label="ZBTB20(NM_001164342)"
                     /note="ZBTB20(NM_001164342)"
                     /gene="ZBTB20"
     CDS             2837..2863
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2909..3250
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3376..3875
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3896..4603
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4604..4660
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4661..5260
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5271..5859
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5922..6155
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6180..6198)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6987..7091
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7092..7952
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8123..8711
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8957..9286
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10420..10485
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10615..10635
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11060..11194
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11363..11996
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12043..12168
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12660..12893
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13078..13122
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctttgcta gcagcagtga
      601 actccaagcc accatgctag aacggaagaa acccaagaca gctgaaaacc agaaggcatc
      661 tgaggagaat gagattactc agccgggtgg atccagcgcc aagccgggcc ttccctgcct
      721 gaactttgaa gctgttttgt ctccagaccc agccctcatc cactcaacac attcactgac
      781 aaactctcac gctcacaccg ggtcatctga ttgtgacatc agttgcaagg ggatgaccga
      841 gcgcattcac agcatcaacc ttcacaactt cagcaattcc gtgctcgaga ccctcaacga
      901 gcagcgcaac cgtggccact tctgtgacgt aacggtgcgc atccacggga gcatgctgcg
      961 cgcacaccgc tgcgtgctgg cagccggcag ccccttcttc caggacaaac tgctgcttgg
     1021 ctacagcgac atcgagatcc cgtcggtggt gtcagtgcag tcagtgcaaa agctcattga
     1081 cttcatgtac agcggcgtgc tacgggtctc gcagtcggaa gctctgcaga tcctcacggc
     1141 cgccagcatc ctgcagatca aaacagtcat cgacgagtgc acgcgcatcg tgtcacagaa
     1201 cgtgggcgat gtgttcccgg ggatccagga ctcgggccag gacacgccgc ggggcactcc
     1261 cgagtcaggc acgtcaggcc agagcagcga cacggagtcg ggctacctgc agagccaccc
     1321 acagcacagc gtggacagga tctactcggc actctacgcg tgctccatgc agaatggcag
     1381 cggcgagcgc tctttttaca gcggcgcagt ggtcagccac cacgagactg cgctcggcct
     1441 gccccgcgac caccacatgg aagaccccag ctggatcaca cgcatccatg agcgctcgca
     1501 gcagatggag cgctacctgt ccaccacccc cgagaccacg cactgccgca agcagccccg
     1561 gcctgtgcgc atccagaccc tagtgggcaa catccacatc aagcaggaga tggaggacga
     1621 ttacgactac tacgggcagc aaagggtgca gatcctggaa cgcaacgaat ccgaggagtg
     1681 cacggaagac acagaccagg ccgagggcac cgagagtgag cccaaaggtg aaagcttcga
     1741 ctcgggcgtc agctcctcca taggcaccga gcctgactcg gtggagcagc agtttgggcc
     1801 tggggcggcg cgggacagcc aggctgaacc cacccaaccc gagcaggctg cagaagcccc
     1861 cgctgagggt ggtccgcaga caaaccagct agaaacaggt gcttcctctc cggagagaag
     1921 caatgaagtg gagatggaca gcactgttat cactgtcagc aacagctccg acaagagcgt
     1981 cctacaacag ccttcggtca acacgtccat cgggcagcca ttgccaagta cccagctcta
     2041 cttacgccag acagaaaccc tcaccagcaa cctgaggatg cctctgacct tgaccagcaa
     2101 cacgcaggtc attggcacag ctggcaacac ctacctgcca gccctcttca ctacccagcc
     2161 cgcgggcagt ggccccaagc ctttcctctt cagcctgcca cagcccctgg caggccagca
     2221 gacccagttt gtgacagtgt cccagcccgg tctgtcgacc tttactgcac agctgccagc
     2281 gccacagccc ctggcctcat ccgcaggcca cagcacagcc agtgggcaag gcgaaaaaaa
     2341 gccttatgag tgcactctct gcaacaagac tttcaccgcc aaacagaact acgtcaagca
     2401 catgttcgta cacacaggtg agaagcccca ccaatgcagc atctgttggc gctccttctc
     2461 cttaaaggat taccttatca agcacatggt gacacacaca ggagtgaggg cataccagtg
     2521 tagtatctgc aacaagcgct tcacccagaa gagctccctc aacgtgcaca tgcgcctcca
     2581 ccggggagag aagtcctacg agtgctacat ctgcaaaaag aagttctctc acaagaccct
     2641 cctggagcga cacgtggccc tgcacagtgc cagcaatggg accccccctg caggcacacc
     2701 cccaggtgcc cgcgctggcc ccccaggcgt ggtggcctgc acggagggga ccacttacgt
     2761 ctgctccgtc tgcccagcaa agtttgacca aatcgagcag ttcaacgacc acatgaggat
     2821 gcatgtgtct gacggatacc cctacgacgt gcctgactac gccggcggcg gtggttcagg
     2881 cggtggcggt tccggtggtg ggggatcagt ccaactggtg gagtctggtg gcgctttggt
     2941 gcagccaggt ggctctctgc gtttgtcctg tgccgcttct ggcttcccag tgaaccgcta
     3001 ttccatgcgc tggtatcgcc aggctccagg caaagagcgt gagtgggtag ccggtatgtc
     3061 cagcgcgggt gatcgtagct cctatgaaga ctccgtgaag ggccgtttca ccatcagccg
     3121 tgacgatgcc cgtaacacgg tgtatctgca aatgaacagc ttgaaacctg aagatacggc
     3181 cgtgtattac tgtaatgtga acgtgggctt cgagtattgg ggccaaggca cccaggtcac
     3241 cgtctccagc taagatcctt gacttgcggc cgcaactccc acctgcaaca tgcgtgactg
     3301 actgaggccg cgactctaga gtcgacctgc atctaggcgc cggaattaga tctctcgagg
     3361 ttaacgaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     3421 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     3481 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     3541 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     3601 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     3661 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     3721 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     3781 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     3841 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatggt
     3901 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     3961 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     4021 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     4081 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     4141 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     4201 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     4261 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     4321 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     4381 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     4441 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     4501 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     4561 cgagggccgc cactccaccg gcggcatgga cgagctgtac aaggcaacaa acttctctct
     4621 gctgaaacaa gccggagatg tcgaagagaa tcctggaccg atgaccgagt acaagcccac
     4681 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     4741 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     4801 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     4861 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     4921 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     4981 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     5041 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     5101 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     5161 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     5221 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga tcttatcgat aatcaacctc
     5281 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5341 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5401 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5461 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5521 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5581 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5641 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     5701 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     5761 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     5821 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgaccaaggc
     5881 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     5941 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     6001 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6061 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6121 cagacccttt tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt
     6181 ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg
     6241 cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc
     6301 ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt
     6361 tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct
     6421 agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     6481 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     6541 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     6601 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     6661 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag
     6721 tacaatctag ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     6781 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     6841 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     6901 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     6961 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7021 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7081 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7141 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7201 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7261 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7321 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7381 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7441 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7501 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7561 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7621 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7681 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7741 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7801 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7861 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7921 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7981 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8041 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8101 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8161 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8221 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     8281 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8341 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8401 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8461 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8521 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8581 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8641 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8701 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8761 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8821 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8881 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     8941 aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     9001 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     9061 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     9121 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     9181 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     9241 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa
     9301 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
     9361 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
     9421 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
     9481 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
     9541 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
     9601 gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
     9661 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
     9721 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc
     9781 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
     9841 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg
     9901 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
     9961 actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc
    10021 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
    10081 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
    10141 agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
    10201 ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga
    10261 gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca
    10321 actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca
    10381 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
    10441 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
    10501 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
    10561 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
    10621 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
    10681 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
    10741 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
    10801 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
    10861 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
    10921 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
    10981 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
    11041 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
    11101 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    11161 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
    11221 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctag tggtttcatt
    11281 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    11341 tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc
    11401 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
    11461 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
    11521 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
    11581 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
    11641 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
    11701 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
    11761 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
    11821 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
    11881 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
    11941 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
    12001 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
    12061 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
    12121 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
    12181 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
    12241 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
    12301 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
    12361 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
    12421 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
    12481 aaaagtaaga ccaccgcaca gcaagcggcc ggtgatcttc agacctggac gatatatatg
    12541 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
    12601 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
    12661 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
    12721 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
    12781 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
    12841 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
    12901 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
    12961 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
    13021 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
    13081 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
    13141 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
    13201 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
    13261 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
    13321 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtcg
//
