LOCUS       V030131                13176 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030131
VERSION     V030131
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2632
                     /label="ZBTB16(NM_006006)"
                     /note="ZBTB16(NM_006006)"
                     /gene="ZBTB16"
     CDS             2633..2659
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2705..3046
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3172..3671
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3692..4399
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4400..4456
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4457..5056
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5067..5655
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5718..5951
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5976..5994)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6783..6887
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6888..7748
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7919..8507
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8753..9082
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10216..10281
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10411..10431
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10856..10990
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11159..11792
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11839..11964
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12456..12689
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12874..12918
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctattgaa aaagcagtga
      601 actccaagcc accatggatc tgacaaaaat gggcatgatc cagctgcaga accctagcca
      661 ccccacgggg ctactgtgca aggccaacca gatgcggctg gccgggactt tgtgcgatgt
      721 ggtcatcatg gtggacagcc aggagttcca cgcccaccgg acggtgctgg cctgcaccag
      781 caagatgttt gagatcctct tccaccgcaa tagtcaacac tatactttgg acttcctctc
      841 gccaaagacc ttccagcaga ttctggagta tgcatataca gccacgctgc aagccaaggc
      901 ggaggacctg gatgacctgc tgtatgcggc cgagatcctg gagatcgagt acctggagga
      961 acagtgcctg aagatgctgg agaccatcca ggcctcagac gacaatgaca cggaggccac
     1021 catggccgat ggcggggccg aggaagaaga ggaccgcaag gctcggtacc tcaagaacat
     1081 cttcatctcg aagcattcca gcgaggagag tgggtatgcc agtgtggctg gacagagcct
     1141 ccctgggccc atggtggacc agagcccttc agtctccact tcatttggtc tttcagccat
     1201 gagtcccacc aaggctgcag tggacagttt gatgaccata ggacagtctc tcctgcaggg
     1261 aactcttcag ccacctgcag ggcccgagga gccaactctg gctgggggtg ggcggcaccc
     1321 tggggtggct gaggtgaaga cggagatgat gcaggtggat gaggtgccca gccaggacag
     1381 ccctggggca gccgagtcca gcatctcagg agggatgggg gacaaggttg aggaaagagg
     1441 caaagagggg cctgggaccc cgactcgaag cagcgtcatc accagtgcta gggagctaca
     1501 ctatgggcga gaggagagtg ccgagcaggt gccaccccca gctgaggctg gccaggcccc
     1561 cactggccga cctgagcacc cagcaccccc gcctgagaag catctgggca tctactccgt
     1621 gttgcccaac cacaaggctg acgctgtatt gagcatgccg tcttccgtga cctctggcct
     1681 ccacgtgcag cctgccctgg ctgtctccat ggacttcagc acctatgggg ggctgctgcc
     1741 ccagggcttc atccagaggg agctgttcag caagctgggg gagctggctg tgggcatgaa
     1801 gtcagagagc cggaccatcg gagagcagtg cagcgtgtgt ggggtcgagc ttcctgataa
     1861 cgaggctgtg gagcagcaca ggaagctgca cagtgggatg aagacgtacg ggtgcgagct
     1921 ctgcgggaag cggttcctgg atagtttgcg gctgagaatg cacttactgg ctcattcagc
     1981 gggtgccaaa gcctttgtct gtgatcagtg cggtgcacag ttttcgaagg aggatgccct
     2041 ggagacacac aggcagaccc atactggcac tgacatggcc gtcttctgtc tgctgtgtgg
     2101 gaagcgcttc caggcgcaga gcgcactgca gcagcacatg gaggtccacg cgggcgtgcg
     2161 cagctacatc tgcagtgagt gcaaccgcac cttccccagc cacacggctc tcaaacgcca
     2221 cctgcgctca catacaggcg accaccccta cgagtgtgag ttctgtggca gctgcttccg
     2281 ggatgagagc acactcaaga gccacaaacg catccacacg ggtgagaaac cctacgagtg
     2341 caatggctgt ggcaagaagt tcagcctcaa gcatcagctg gagacgcact atagggtgca
     2401 cacaggtgag aagccctttg agtgtaagct ctgccaccag cgctcccggg actactcggc
     2461 catgatcaag cacctgagaa cgcacaacgg cgcctcgccc taccagtgca ccatctgcac
     2521 agagtactgc cccagcctct cctccatgca gaagcacatg aagggccaca agcccgagga
     2581 gatcccgccc gactggagga tagagaagac gtacctctac ctgtgctatg tgtaccccta
     2641 cgacgtgcct gactacgccg gcggcggtgg ttcaggcggt ggcggttccg gtggtggggg
     2701 atcagtccaa ctggtggagt ctggtggcgc tttggtgcag ccaggtggct ctctgcgttt
     2761 gtcctgtgcc gcttctggct tcccagtgaa ccgctattcc atgcgctggt atcgccaggc
     2821 tccaggcaaa gagcgtgagt gggtagccgg tatgtccagc gcgggtgatc gtagctccta
     2881 tgaagactcc gtgaagggcc gtttcaccat cagccgtgac gatgcccgta acacggtgta
     2941 tctgcaaatg aacagcttga aacctgaaga tacggccgtg tattactgta atgtgaacgt
     3001 gggcttcgag tattggggcc aaggcaccca ggtcaccgtc tccagctaag atccttgact
     3061 tgcggccgca actcccacct gcaacatgcg tgactgactg aggccgcgac tctagagtcg
     3121 acctgcatct aggcgccgga attagatctc tcgaggttaa cgaattctac cgggtagggg
     3181 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     3241 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     3301 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     3361 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     3421 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     3481 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     3541 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     3601 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     3661 cgggcctttc gacctgcagc ccaagcttac catggtgagc aagggcgagg aggataacat
     3721 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     3781 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     3841 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     3901 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     3961 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     4021 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     4081 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     4141 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     4201 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     4261 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     4321 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     4381 catggacgag ctgtacaagg caacaaactt ctctctgctg aaacaagccg gagatgtcga
     4441 agagaatcct ggaccgatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     4501 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     4561 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     4621 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     4681 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     4741 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     4801 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     4861 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     4921 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     4981 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     5041 caagcccggt gcctgatctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     5101 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     5161 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     5221 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     5281 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     5341 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     5401 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     5461 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     5521 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     5581 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     5641 gggccgcctc cccgcatcga taccgtcgac caaggcagct gtagatctta gccacttttt
     5701 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     5761 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     5821 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     5881 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     5941 aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg
     6001 tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag
     6061 cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc
     6121 gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg
     6181 gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc
     6241 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     6301 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     6361 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     6421 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     6481 tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctagctct gatgccgcat
     6541 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     6601 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     6661 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     6721 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     6781 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6841 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6901 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6961 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7021 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7081 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     7141 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     7201 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7261 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7321 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7381 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     7441 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7501 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7561 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7621 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7681 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7741 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7801 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7861 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7921 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7981 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8041 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     8101 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8161 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8221 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8281 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8341 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8401 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8461 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8521 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8581 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8641 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     8701 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca
     8761 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8821 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8881 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     8941 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     9001 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     9061 tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga
     9121 gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg
     9181 aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga
     9241 acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg
     9301 tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg
     9361 aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc
     9421 gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa
     9481 ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat
     9541 tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc
     9601 gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt
     9661 tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc
     9721 gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa
     9781 cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg
     9841 caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag
     9901 tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca
     9961 tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac
    10021 gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg
    10081 atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg
    10141 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
    10201 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
    10261 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
    10321 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
    10381 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
    10441 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
    10501 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
    10561 atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca taacatactg
    10621 ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg
    10681 tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc
    10741 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
    10801 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
    10861 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    10921 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    10981 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
    11041 ccctattacc actgccaatt acctagtggt ttcatttact ctaaacctgt gattcctctg
    11101 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg
    11161 gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca
    11221 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
    11281 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
    11341 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
    11401 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
    11461 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
    11521 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
    11581 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
    11641 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
    11701 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    11761 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
    11821 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
    11881 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
    11941 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
    12001 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
    12061 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
    12121 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
    12181 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
    12241 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
    12301 gcggccggtg atcttcagac ctggacgata tatatgaggg acaattggag aagtgaatta
    12361 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
    12421 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
    12481 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
    12541 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
    12601 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
    12661 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
    12721 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
    12781 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
    12841 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
    12901 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
    12961 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
    13021 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
    13081 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
    13141 agatccattc gattagtgaa cggatctcga cggtcg
//
