LOCUS       V030049                13296 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030049
VERSION     V030049
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2752
                     /label="ZBTB1(NM_001123329)"
                     /note="ZBTB1(NM_001123329)"
                     /gene="ZBTB1"
     CDS             2753..2779
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2825..3166
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3292..3791
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3812..4519
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4520..4576
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4577..5176
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5187..5775
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5838..6071
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6096..6114)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6903..7007
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7008..7868
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8039..8627
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8873..9202
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10336..10401
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10531..10551
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10976..11110
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11279..11912
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11959..12084
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12576..12809
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12994..13038
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctccgtcc ctagcagtga
      601 actccaagcc accatggcaa agcccagcca cagcagctat gtccttcagc agctaaacaa
      661 ccaaagagaa tggggttttc tctgtgactg ctgtattgca attgatgaca tttactttca
      721 agcacacaag gcagttctag ctgcctgtag ctcctatttt agaatgtttt tcatgaacca
      781 tcagcatagt actgcacaac tgaatctcag caacatgaaa attagtgcag aatgttttga
      841 tctcattttg cagtttatgt atttaggaaa aattatgaca gctccctcca gttttgagca
      901 gtttaaagtg gcaatgaact acctacagct atacaatgtt cctgactgtt tagaagacat
      961 ccaggatgca gattgttcta gttcaaaatg ttcctcttct gcttccagca aacagaacag
     1021 caaaatgata tttggggtaa gaatgtatga agatactgtg gctcgaaatg gcaatgaagc
     1081 caacaggtgg tgtgcagagc caagttcaac ggtaaataca ccacataata gagaggctga
     1141 tgaagagtct ttacaattag gtaattttcc tgagccacta tttgatgtat gtaaaaaaag
     1201 ttccgtgtcc aaattatcta ctccaaaaga acgtgtgtca agacgctttg ggcggagttt
     1261 tacctgtgat agctgtggat ttggctttag ctgtgaaaaa ttattagatg agcatgtgct
     1321 aacctgtact aacagacatt tataccaaaa cacaagatct taccatagaa tagtagatat
     1381 tagagatgga aaagacagta acatcaaagc tgaatttggt gaaaaagatt cttccaaaac
     1441 attttctgca cagacggaca aatacagagg agacacaagc caggctgctg atgattcagc
     1501 ttcaaccact ggaagcagaa aaagtagcac agtggagtct gaaatagcaa gcgaagagaa
     1561 aagcagagct gctgagagga aaaggattat tattaagatg gagccagaag atattcctac
     1621 agatgaactg aaagacttta acattattaa agttactgat aaagactgta atgaatccac
     1681 tgacaatgat gaattagaag atgaacctga agagccattt tatagatact atgttgaaga
     1741 agatgtcagc ataaaaaaaa gtggtaggaa aactctaaaa cctcgaatgt cagtaagtgc
     1801 tgatgaaaga ggtggtttag agaatatgag gccccctaac aacagcagtc cagtacaaga
     1861 ggatgctgaa aatgcatctt gtgagctgtg tggacttaca ataaccgagg aggacctgtc
     1921 atctcattac ttagccaaac acattgaaaa tatctgtgca tgtggtaaat gtggacaaat
     1981 acttgtaaag ggtaggcagc ttcaggaaca tgctcaacga tgtggcgagc cccaagatct
     2041 gaccatgaat gggttaggaa atactgagga gaaaatggac ttggaagaga atcctgatga
     2101 gcagtccgaa ataagagata tgtttgttga aatgctggat gattttaggg acaatcatta
     2161 ccagataaac agtatccaaa aaaagcagtt atttaaacat tctgcctgcc cttttcgatg
     2221 tcctaattgt ggccagcgtt ttgaaactga aaatctagtg gttgaacata tgtctagctg
     2281 cttagatcaa gatatgttta agagtgccat catggaagaa aatgaaagag atcacagacg
     2341 aaagcatttt tgtaatctgt gtggaaaagg attttatcag cggtgtcact taagagaaca
     2401 ctatactgtt catactaagg aaaaacagtt tgtttgtcaa acatgtggaa agcagttttt
     2461 aagagagcgt cagttgcgac tgcacaatga tatgcacaaa ggcatggcca ggtatgtctg
     2521 ttccatttgt gatcaaggaa acttcagaaa acatgaccat gtacggcata tgatttctca
     2581 tttatctgct ggtgagacta tatgccaggt ctgctttcag atattcccaa ataatgaaca
     2641 gttggagcag cacatggatg ttcatctgta tacatgtgga atatgtggag caaaatttaa
     2701 tttgaggaaa gatatgagat cacattataa tgccaagcat ttgaaaagaa cctaccccta
     2761 cgacgtgcct gactacgccg gcggcggtgg ttcaggcggt ggcggttccg gtggtggggg
     2821 atcagtccaa ctggtggagt ctggtggcgc tttggtgcag ccaggtggct ctctgcgttt
     2881 gtcctgtgcc gcttctggct tcccagtgaa ccgctattcc atgcgctggt atcgccaggc
     2941 tccaggcaaa gagcgtgagt gggtagccgg tatgtccagc gcgggtgatc gtagctccta
     3001 tgaagactcc gtgaagggcc gtttcaccat cagccgtgac gatgcccgta acacggtgta
     3061 tctgcaaatg aacagcttga aacctgaaga tacggccgtg tattactgta atgtgaacgt
     3121 gggcttcgag tattggggcc aaggcaccca ggtcaccgtc tccagctaag atccttgact
     3181 tgcggccgca actcccacct gcaacatgcg tgactgactg aggccgcgac tctagagtcg
     3241 acctgcatct aggcgccgga attagatctc tcgaggttaa cgaattctac cgggtagggg
     3301 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     3361 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     3421 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     3481 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     3541 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     3601 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     3661 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     3721 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     3781 cgggcctttc gacctgcagc ccaagcttac catggtgagc aagggcgagg aggataacat
     3841 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     3901 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     3961 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     4021 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     4081 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     4141 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     4201 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     4261 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     4321 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     4381 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     4441 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     4501 catggacgag ctgtacaagg caacaaactt ctctctgctg aaacaagccg gagatgtcga
     4561 agagaatcct ggaccgatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     4621 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     4681 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     4741 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     4801 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     4861 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     4921 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     4981 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     5041 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     5101 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     5161 caagcccggt gcctgatctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     5221 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     5281 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     5341 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     5401 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     5461 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     5521 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     5581 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     5641 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     5701 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     5761 gggccgcctc cccgcatcga taccgtcgac caaggcagct gtagatctta gccacttttt
     5821 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     5881 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     5941 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6001 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6061 aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg
     6121 tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag
     6181 cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc
     6241 gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg
     6301 gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc
     6361 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     6421 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     6481 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     6541 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     6601 tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctagctct gatgccgcat
     6661 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     6721 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     6781 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     6841 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     6901 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6961 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7021 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7081 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7141 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7201 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     7261 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     7321 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7381 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7441 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7501 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     7561 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7621 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7681 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7741 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7801 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7861 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7921 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7981 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8041 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8101 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8161 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     8221 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8281 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8341 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8401 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8461 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8521 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8581 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8641 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8701 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8761 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     8821 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca
     8881 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8941 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     9001 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     9061 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     9121 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     9181 tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga
     9241 gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg
     9301 aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga
     9361 acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg
     9421 tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg
     9481 aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc
     9541 gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa
     9601 ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat
     9661 tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc
     9721 gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt
     9781 tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc
     9841 gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa
     9901 cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg
     9961 caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag
    10021 tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca
    10081 tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac
    10141 gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg
    10201 atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg
    10261 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
    10321 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
    10381 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
    10441 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
    10501 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
    10561 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
    10621 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
    10681 atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca taacatactg
    10741 ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg
    10801 tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc
    10861 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
    10921 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
    10981 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    11041 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    11101 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
    11161 ccctattacc actgccaatt acctagtggt ttcatttact ctaaacctgt gattcctctg
    11221 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg
    11281 gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca
    11341 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
    11401 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
    11461 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
    11521 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
    11581 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
    11641 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
    11701 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
    11761 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
    11821 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    11881 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
    11941 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
    12001 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
    12061 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
    12121 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
    12181 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
    12241 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
    12301 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
    12361 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
    12421 gcggccggtg atcttcagac ctggacgata tatatgaggg acaattggag aagtgaatta
    12481 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
    12541 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
    12601 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
    12661 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
    12721 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
    12781 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
    12841 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
    12901 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
    12961 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
    13021 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
    13081 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
    13141 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
    13201 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
    13261 agatccattc gattagtgaa cggatctcga cggtcg
//
