LOCUS       V030142                13923 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030142
VERSION     V030142
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..3379
                     /label="WWP1(NM_007013)"
                     /note="WWP1(NM_007013)"
                     /gene="WWP1"
     CDS             3380..3406
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3452..3793
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3919..4418
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4439..5146
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5147..5203
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             5204..5803
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5814..6402
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6465..6698
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6723..6741)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7530..7634
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7635..8495
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8666..9254
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9500..9829
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10963..11028
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             11158..11178
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11603..11737
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11906..12539
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12586..12711
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    13203..13436
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13621..13665
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctgttaga cgcgcagtga
      601 actccaagcc accatggcca ctgcttcacc aaggtctgat actagtaata accacagtgg
      661 aaggttgcag ttacaggtaa ctgtttctag tgccaaactt aaaagaaaaa agaactggtt
      721 cggaacagca atatatacag aagtagttgt agatggagaa attacgaaaa cagcaaaatc
      781 cagtagttct tctaatccaa aatgggatga acagctaact gtaaatgtta cgccacagac
      841 tacattggaa tttcaagttt ggagccatcg cactttaaaa gcagatgctt tattaggaaa
      901 agcaacgata gatttgaaac aagctctgtt gatacacaat agaaaattgg aaagagtgaa
      961 agaacaatta aaactttcct tggaaaacaa gaatggcata gcacaaactg gtgaattgac
     1021 agttgtgctt gatggattgg tgattgagca agaaaatata acaaactgca gctcatctcc
     1081 aaccatagaa atacaggaaa atggtgatgc cttacatgaa aatggagagc cttcagcaag
     1141 gacaactgcc aggttggctg ttgaaggcac gaatggaata gataatcatg tacctacaag
     1201 cactctagtc caaaactcat gctgctcgta tgtagttaat ggagacaaca caccttcatc
     1261 tccgtctcag gttgctgcca gacccaaaaa tacaccagct ccaaaaccac tcgcatctga
     1321 gcctgccgat gacactgtta atggagaatc atcctcattt gcaccaactg ataatgcgtc
     1381 tgtcacgggt actccagtag tgtctgaaga aaatgccttg tctccaaatt gcactagtac
     1441 tactgttgaa gatcctccag ttcaagaaat actgacttcc tcagaaaaca atgaatgtat
     1501 tccttctacc agtgcagaat tggaatctga agctagaagt atattagagc ctgacacctc
     1561 taattctaga agtagttctg cttttgaagc agccaaatca agacagccag atgggtgtat
     1621 ggatcctgta cggcagcagt ctgggaatgc caacacagaa accttgccat cagggtggga
     1681 acaaagaaaa gatcctcatg gtagaaccta ttatgtggat cataatactc gaactaccac
     1741 atgggagaga ccacaacctt tacctccagg ttgggaaaga agagttgatg atcgtagaag
     1801 agtttattat gtggatcata acaccagaac aacaacgtgg cagcggccta ccatggaatc
     1861 tgtccgaaat tttgaacagt ggcaatctca gcggaaccaa ttgcagggag ctatgcaaca
     1921 gtttaaccaa cgatacctct attcggcttc aatgttagct gcagaaaatg acccttatgg
     1981 acctttgcca ccaggctggg aaaaaagagt ggattcaaca gacagggttt actttgtgaa
     2041 tcataacaca aaaacaaccc agtgggaaga tccaagaact caaggcttac agaatgaaga
     2101 acccctgcca gaaggctggg aaattagata tactcgtgaa ggtgtaaggt actttgttga
     2161 tcataacaca agaacaacaa cattcaaaga tcctcgcaat gggaagtcat ctgtaactaa
     2221 aggtggtcca caaattgctt atgaacgcgg ctttaggtgg aagcttgctc acttccgtta
     2281 tttgtgccag tctaatgcac tacctagtca tgtaaagatc aatgtgtccc ggcagacatt
     2341 gtttgaagat tccttccaac agattatggc attaaaaccc tatgacttga ggaggcgctt
     2401 atatgtaata tttagaggag aagaaggact tgattatggt ggcctagcga gagaatggtt
     2461 tttcttgctt tcacatgaag ttttgaaccc aatgtattgc ttatttgagt atgcgggcaa
     2521 gaacaactat tgtctgcaga taaatccagc atcaaccatt aatccagacc atctttcata
     2581 cttctgtttc attggtcgtt ttattgccat ggcactattt catggaaagt ttatcgatac
     2641 tggtttctct ttaccattct acaagcgtat gttaagtaaa aaacttacta ttaaggattt
     2701 ggaatctatt gatactgaat tttataactc ccttatctgg ataagagata acaacattga
     2761 agaatgtggc ttagaaatgt acttttctgt tgacatggag attttgggaa aagttacttc
     2821 acatgacctg aagttgggag gttccaatat tctggtgact gaggagaaca aagatgaata
     2881 tattggttta atgacagaat ggcgtttttc tcgaggagta caagaacaga ccaaagcttt
     2941 ccttgatggt tttaatgaag ttgttcctct tcagtggcta cagtacttcg atgaaaaaga
     3001 attagaggtt atgttgtgtg gcatgcagga ggttgacttg gcagattggc agagaaatac
     3061 tgtttatcga cattatacaa gaaacagcaa gcaaatcatt tggttttggc agtttgtgaa
     3121 agagacagac aatgaagtaa gaatgcgact attgcagttc gtcactggaa cctgccgttt
     3181 acctctagga ggatttgctg agctcatggg aagtaatggg cctcaaaagt tttgcattga
     3241 aaaagttggc aaagacactt ggttaccaag aagccataca tgttttaatc gcttggatct
     3301 accaccatat aagagttatg aacaactaaa ggaaaaactt ctttttgcaa tagaagagac
     3361 agagggattt ggacaagaat acccctacga cgtgcctgac tacgccggcg gcggtggttc
     3421 aggcggtggc ggttccggtg gtgggggatc agtccaactg gtggagtctg gtggcgcttt
     3481 ggtgcagcca ggtggctctc tgcgtttgtc ctgtgccgct tctggcttcc cagtgaaccg
     3541 ctattccatg cgctggtatc gccaggctcc aggcaaagag cgtgagtggg tagccggtat
     3601 gtccagcgcg ggtgatcgta gctcctatga agactccgtg aagggccgtt tcaccatcag
     3661 ccgtgacgat gcccgtaaca cggtgtatct gcaaatgaac agcttgaaac ctgaagatac
     3721 ggccgtgtat tactgtaatg tgaacgtggg cttcgagtat tggggccaag gcacccaggt
     3781 caccgtctcc agctaagatc cttgacttgc ggccgcaact cccacctgca acatgcgtga
     3841 ctgactgagg ccgcgactct agagtcgacc tgcatctagg cgccggaatt agatctctcg
     3901 aggttaacga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     3961 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     4021 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     4081 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     4141 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     4201 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     4261 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     4321 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     4381 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     4441 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     4501 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     4561 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     4621 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     4681 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     4741 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     4801 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     4861 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     4921 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     4981 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     5041 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     5101 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaaggcaa caaacttctc
     5161 tctgctgaaa caagccggag atgtcgaaga gaatcctgga ccgatgaccg agtacaagcc
     5221 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     5281 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     5341 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     5401 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     5461 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     5521 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     5581 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     5641 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     5701 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     5761 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgatcttatc gataatcaac
     5821 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     5881 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     5941 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     6001 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     6061 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     6121 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6181 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     6241 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     6301 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     6361 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgaccaa
     6421 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     6481 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     6541 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6601 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6661 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt
     6721 attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag
     6781 ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag
     6841 accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga
     6901 gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc
     6961 gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     7021 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     7081 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     7141 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     7201 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct
     7261 cagtacaatc tagctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     7321 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     7381 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     7441 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     7501 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7561 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7621 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7681 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7741 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7801 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7861 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7921 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7981 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8041 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8101 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8161 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8221 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8281 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8341 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8401 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8461 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8521 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8581 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8641 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8701 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8761 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     8821 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8881 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8941 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9001 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9061 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9121 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9181 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9241 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9301 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9361 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9421 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     9481 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     9541 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     9601 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     9661 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     9721 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     9781 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca
     9841 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
     9901 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
     9961 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
    10021 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta
    10081 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
    10141 gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt
    10201 ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga
    10261 aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt
    10321 tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg
    10381 tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc
    10441 gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct
    10501 ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta
    10561 ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct
    10621 ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg
    10681 tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga
    10741 tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga
    10801 tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg
    10861 gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg
    10921 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
    10981 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
    11041 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
    11101 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
    11161 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
    11221 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
    11281 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
    11341 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
    11401 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
    11461 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
    11521 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
    11581 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
    11641 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    11701 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    11761 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tagtggtttc
    11821 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    11881 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
    11941 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
    12001 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
    12061 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
    12121 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
    12181 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
    12241 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
    12301 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
    12361 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    12421 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    12481 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    12541 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
    12601 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
    12661 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
    12721 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
    12781 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
    12841 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
    12901 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
    12961 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
    13021 aacaaaagta agaccaccgc acagcaagcg gccggtgatc ttcagacctg gacgatatat
    13081 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
    13141 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
    13201 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
    13261 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
    13321 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
    13381 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
    13441 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
    13501 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
    13561 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    13621 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    13681 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    13741 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
    13801 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
    13861 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
    13921 tcg
//
