LOCUS       V030213                13719 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030213
VERSION     V030213
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..3175
                     /label="VPS41(NM_014396)"
                     /note="VPS41(NM_014396)"
                     /gene="VPS41"
     CDS             3176..3202
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3248..3589
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3715..4214
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4235..4942
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4943..4999
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             5000..5599
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5610..6198
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6261..6494
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6519..6537)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7326..7430
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7431..8291
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8462..9050
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9296..9625
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10759..10824
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10954..10974
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11399..11533
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11702..12335
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12382..12507
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12999..13232
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13417..13461
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctgctata ggtgcagtga
      601 actccaagcc accatggcgg aagcagagga gcaggaaact gggtcccttg aagaatctac
      661 agatgagtct gaggaagaag agagcgaaga ggaacccaag ctgaagtatg aaaggctttc
      721 caatggggta actgaaatac ttcagaagga tgcagctagc tgcatgacag tccatgacaa
      781 gtttttggca ttgggcacac attatggcaa ggtttattta cttgatgtcc aggggaacat
      841 cactcagaag tttgatgtaa gtcctgtgaa gataaatcag attagcttgg atgaaagtgg
      901 agagcacatg ggtgtgtgtt cagaggatgg caaggtgcag gtatttggac tgtattctgg
      961 agaagaattt cacgagactt ttgactgtcc cattaaaatt attgctgtgc acccacattt
     1021 cgtgagatcc agttgcaagc agtttgtgac cggagggaag aagctgctac tgtttgaacg
     1081 gtcttggatg aacagatgga agtctgctgt tctgcatgaa ggggaaggga acataaggag
     1141 tgtgaagtgg agaggccatc tgattgcttg ggccaataat atgggtgtga agatttttga
     1201 catcatctca aagcaaagaa tcaccaatgt gccccgggat gatataagtc ttcgcccaga
     1261 catgtatccc tgcagcctct gctggaagga caatgtgaca ctgattattg gctgggggac
     1321 ttctgtcaag gtgtgctcag tgaaggaacg gcatgccagt gaaatgaggg atttgccaag
     1381 tcgatatgtt gaaatagtgt ctcagtttga aactgaattc tacatcagtg gacttgcacc
     1441 tctctgtgat cagcttgttg tactttcgta tgtaaaggag atttcagaaa aaacggaaag
     1501 agaatactgt gccaggccta gactggacat catccagcca ctttctgaga cttgtgaaga
     1561 gatctcttct gatgctttga cagtcagagg ctttcaggag aatgaatgta gagattatca
     1621 tttagaatac tctgaagggg aatcactttt ttacatcgtg agtccgagag atgttgtagt
     1681 ggccaaggaa cgagaccaag atgatcacat tgactggctc cttgaaaaga agaaatatga
     1741 agaagcattg atggcagctg aaattagcca aaaaaatatt aaaagacata agattctgga
     1801 tattggcttg gcatatataa atcacctggt ggagagagga gactatgaca tagcagcacg
     1861 caaatgccag aaaattcttg ggaaaaatgc agcactctgg gaatatgaag tttataaatt
     1921 taaagaaatt ggacagctta aggctattag tccttatttg ccaagaggtg atccagttct
     1981 gaaaccactc atctatgaaa tgatcttaca tgaatttttg gagagtgatt atgagggttt
     2041 tgccacattg atccgagaat ggcctggaga tctgtataat aattcagtca tagttcaagc
     2101 agttcgggat catttgaaga aagatagtca gaacaagact ttacttaaaa ccctggcaga
     2161 attgtacacc tatgacaaga actatggcaa tgctctggaa atatacttaa cattaagaca
     2221 taaagacgtt tttcagttga tccacaagca taatcttttc agttctatca aggataaaat
     2281 tgttttatta atggattttg attcagagaa agctgttgac atgcttttgg acaatgaaga
     2341 taaaatttca attaaaaagg tagtggaaga attggaagac agaccagagc tacagcatgt
     2401 gtatttgcat aagcttttca agagagacca ccataagggg cagcgttacc atgaaaaaca
     2461 gatcagtctt tatgctgaat atgatcgacc aaacttactt ccctttctcc gagacagtac
     2521 ccattgccca cttgaaaagg ctcttgagat ctgtcaacag agaaactttg tagaagagac
     2581 agtttatctt ctgagccgaa tgggtaatag ccgaagtgcc ctgaagatga ttatggagga
     2641 attacatgat gttgataaag caatcgaatt tgccaaggag caagatgatg gagagctgtg
     2701 ggaagatttg attttatatt ccattgacaa accaccattt attactggct tgttaaacaa
     2761 cattggcaca catgttgacc caattctact gattcaccgt attaaggaag gaatggagat
     2821 ccccaatttg agagattcct tggttaaaat tctgcaagac tacaatttgc aaattctgct
     2881 tcgtgaaggc tgcaagaaga ttctcgtagc tgactctttg tccttactga agaaaatgca
     2941 ccgaactcaa atgaaaggtg ttcttgttga tgaggagaac atctgtgagt cgtgcctttc
     3001 ccctattctt ccatcagatg cagctaagcc cttcagcgtg gtggtcttcc attgccggca
     3061 catgttccac aaggagtgcc tgcccatgcc cagcatgaac tctgctgcac agttctgcaa
     3121 catctgcagt gctaagaacc gtggaccagg aagtgcaatt ttggagatga aaaaataccc
     3181 ctacgacgtg cctgactacg ccggcggcgg tggttcaggc ggtggcggtt ccggtggtgg
     3241 gggatcagtc caactggtgg agtctggtgg cgctttggtg cagccaggtg gctctctgcg
     3301 tttgtcctgt gccgcttctg gcttcccagt gaaccgctat tccatgcgct ggtatcgcca
     3361 ggctccaggc aaagagcgtg agtgggtagc cggtatgtcc agcgcgggtg atcgtagctc
     3421 ctatgaagac tccgtgaagg gccgtttcac catcagccgt gacgatgccc gtaacacggt
     3481 gtatctgcaa atgaacagct tgaaacctga agatacggcc gtgtattact gtaatgtgaa
     3541 cgtgggcttc gagtattggg gccaaggcac ccaggtcacc gtctccagct aagatccttg
     3601 acttgcggcc gcaactccca cctgcaacat gcgtgactga ctgaggccgc gactctagag
     3661 tcgacctgca tctaggcgcc ggaattagat ctctcgaggt taacgaattc taccgggtag
     3721 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     3781 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     3841 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     3901 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     3961 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     4021 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     4081 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     4141 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     4201 ctccgggcct ttcgacctgc agcccaagct taccatggtg agcaagggcg aggaggataa
     4261 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     4321 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     4381 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     4441 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     4501 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     4561 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     4621 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     4681 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     4741 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     4801 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     4861 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     4921 cggcatggac gagctgtaca aggcaacaaa cttctctctg ctgaaacaag ccggagatgt
     4981 cgaagagaat cctggaccga tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     5041 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     5101 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     5161 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     5221 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     5281 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     5341 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     5401 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     5461 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     5521 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     5581 ccgcaagccc ggtgcctgat cttatcgata atcaacctct ggattacaaa atttgtgaaa
     5641 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5701 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5761 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5821 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     5881 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     5941 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     6001 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     6061 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     6121 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     6181 tttgggccgc ctccccgcat cgataccgtc gaccaaggca gctgtagatc ttagccactt
     6241 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     6301 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6361 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6421 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6481 gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt
     6541 atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac
     6601 aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact
     6661 gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac
     6721 ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga
     6781 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     6841 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     6901 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
     6961 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     7021 gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctagc tctgatgccg
     7081 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     7141 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     7201 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     7261 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     7321 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7381 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7441 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7501 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7561 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7621 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     7681 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7741 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     7801 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     7861 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     7921 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     7981 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8041 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8101 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8161 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8221 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8281 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     8341 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8401 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8461 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8521 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8581 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     8641 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8701 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     8761 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     8821 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     8881 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     8941 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     9001 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     9061 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     9121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9181 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     9241 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
     9301 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9361 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9421 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     9481 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     9541 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     9601 ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt
     9661 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
     9721 gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct
     9781 cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca
     9841 tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt
     9901 ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact
     9961 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
    10021 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
    10081 tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa
    10141 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
    10201 tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt
    10261 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
    10321 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
    10381 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
    10441 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa
    10501 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc
    10561 cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg
    10621 atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc
    10681 cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
    10741 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
    10801 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
    10861 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
    10921 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
    10981 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
    11041 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
    11101 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
    11161 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
    11221 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
    11281 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
    11341 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
    11401 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    11461 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    11521 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
    11581 ataccctatt accactgcca attacctagt ggtttcattt actctaaacc tgtgattcct
    11641 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    11701 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
    11761 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
    11821 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
    11881 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
    11941 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
    12001 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
    12061 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
    12121 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
    12181 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    12241 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    12301 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
    12361 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
    12421 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
    12481 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
    12541 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
    12601 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
    12661 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
    12721 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
    12781 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
    12841 caagcggccg gtgatcttca gacctggacg atatatatga gggacaattg gagaagtgaa
    12901 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
    12961 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
    13021 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
    13081 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
    13141 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
    13201 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
    13261 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
    13321 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
    13381 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
    13441 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
    13501 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    13561 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    13621 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
    13681 gacagatcca ttcgattagt gaacggatct cgacggtcg
//
