LOCUS       V033215                13575 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033215
VERSION     V033215
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..3031
                     /label="UHRF1(NM_013282)"
                     /note="UHRF1(NM_013282)"
                     /gene="UHRF1"
     CDS             3032..3058
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3104..3445
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3571..4070
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4091..4798
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4799..4855
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4856..5455
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5466..6054
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6117..6350
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6375..6393)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7182..7286
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7287..8147
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8318..8906
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9152..9481
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10615..10680
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10810..10830
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11255..11389
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11558..12191
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12238..12363
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12855..13088
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13273..13317
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctcaggat tcagcagtga
      601 actccaagcc accatggggg tttttgctgt ccctcccctc agcgccgaca ccatgtggat
      661 ccaggttcgg accatggacg ggaggcagac ccacacggtg gactcgctgt ccaggctgac
      721 caaggtggag gagctgaggc ggaagatcca ggagctgttc cacgtggagc caggcctgca
      781 gaggctgttc tacaggggca aacagatgga ggacggccat accctcttcg actacgaggt
      841 ccgcctgaat gacaccatcc agctcctggt ccgccagagc ctcgtgctcc cccacagcac
      901 caaggagcgg gactccgagc tctccgacac cgactccggc tgctgcctgg gccagagtga
      961 gtcagacaag tcctccaccc acggtgaggc ggccgccgag actgacagca ggccagccga
     1021 tgaggacatg tgggatgaga cggaattggg gctgtacaag gtcaatgagt acgtcgatgc
     1081 tcgggacacg aacatggggg cgtggtttga ggcgcaggtg gtcagggtga cgcggaaggc
     1141 cccctcccgg gacgagccct gcagctccac gtccaggccg gcgctggagg aggacgtcat
     1201 ttaccacgtg aaatacgacg actacccgga gaacggcgtg gtccagatga actccaggga
     1261 cgtccgagcg cgcgcccgca ccatcatcaa gtggcaggac ctggaggtgg gccaggtggt
     1321 catgctcaac tacaaccccg acaaccccaa ggagcggggc ttctggtacg acgcggagat
     1381 ctccaggaag cgcgagacca ggacggcgcg ggaactctac gccaacgtgg tgctggggga
     1441 tgattctctg aacgactgtc ggatcatctt cgtggacgaa gtcttcaaga ttgagcggcc
     1501 gggtgaaggg agccccatgg ttgacaaccc catgagacgg aagagcgggc cgtcctgcaa
     1561 gcactgcaag gacgacgtga acagactctg ccgggtctgc gcctgccacc tgtgcggggg
     1621 ccggcaggac cccgacaagc agctcatgtg cgatgagtgc gacatggcct tccacatcta
     1681 ctgcctggac ccgcccctca gcagtgttcc cagcgaggac gagtggtact gccctgagtg
     1741 ccggaatgat gccagcgagg tggtactggc gggagagcgg ctgagagaga gcaagaagaa
     1801 ggcgaagatg gcctcggcca catcgtcctc acagcgggac tggggcaagg gcatggcctg
     1861 tgtgggccgc accaaggaat gtaccatcgt cccgtccaac cactacggac ccatcccggg
     1921 gatccccgtg ggcaccatgt ggcggttccg agtccaggtc agcgagtcgg gtgtccatcg
     1981 gccccacgtg gctggcatac acggccggag caacgacgga gcgtactccc tagtcctggc
     2041 ggggggctat gaggatgatg tggaccatgg gaattttttc acatacacgg gtagtggtgg
     2101 tcgagatctt tccggcaaca agaggaccgc ggaacagtct tgtgatcaga aactcaccaa
     2161 caccaacagg gcgctggctc tcaactgctt tgctcccatc aatgaccaag aaggggccga
     2221 ggccaaggac tggcggtcgg ggaagccggt cagggtggtg cgcaatgtca agggtggcaa
     2281 gaatagcaag tacgcccccg ctgagggcaa ccgctacgat ggcatctaca aggttgtgaa
     2341 atactggccc gagaagggga agtccgggtt tctcgtgtgg cgctaccttc tgcggaggga
     2401 cgatgatgag cctggccctt ggacgaagga ggggaaggac cggatcaaga agctggggct
     2461 gaccatgcag tatccagaag gctacctgga agccctggcc aaccgagagc gagagaagga
     2521 gaacagcaag agggaggagg aggagcagca ggaggggggc ttcgcgtccc ccaggacggg
     2581 caagggcaag tggaagcgga agtcggcagg aggtggcccg agcagggccg ggtccccgcg
     2641 ccggacatcc aagaaaacca aggtggagcc ctacagtctc acggcccagc agagcagcct
     2701 catcagagag gacaagagca acgccaagct gtggaatgag gtcctggcgt cactcaagga
     2761 ccggccggcg agcggcagcc cgttccagtt gttcctgagt aaagtggagg agacgttcca
     2821 gtgtatctgc tgtcaggagc tggtgttccg gcccatcacg accgtgtgcc agcacaacgt
     2881 gtgcaaggac tgcctggaca gatcctttcg ggcacaggtg ttcagctgcc ctgcctgccg
     2941 ctacgacctg ggccgcagct atgccatgca ggtgaaccag cctctgcaga ccgtcctcaa
     3001 ccagctcttc cccggctacg gcaatggccg gtacccctac gacgtgcctg actacgccgg
     3061 cggcggtggt tcaggcggtg gcggttccgg tggtggggga tcagtccaac tggtggagtc
     3121 tggtggcgct ttggtgcagc caggtggctc tctgcgtttg tcctgtgccg cttctggctt
     3181 cccagtgaac cgctattcca tgcgctggta tcgccaggct ccaggcaaag agcgtgagtg
     3241 ggtagccggt atgtccagcg cgggtgatcg tagctcctat gaagactccg tgaagggccg
     3301 tttcaccatc agccgtgacg atgcccgtaa cacggtgtat ctgcaaatga acagcttgaa
     3361 acctgaagat acggccgtgt attactgtaa tgtgaacgtg ggcttcgagt attggggcca
     3421 aggcacccag gtcaccgtct ccagctaaga tccttgactt gcggccgcaa ctcccacctg
     3481 caacatgcgt gactgactga ggccgcgact ctagagtcga cctgcatcta ggcgccggaa
     3541 ttagatctct cgaggttaac gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     3601 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     3661 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     3721 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     3781 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     3841 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     3901 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     3961 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     4021 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
     4081 caagcttacc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     4141 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     4201 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     4261 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     4321 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     4381 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     4441 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     4501 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     4561 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     4621 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     4681 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     4741 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggc
     4801 aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg gaccgatgac
     4861 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     4921 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     4981 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     5041 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     5101 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     5161 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     5221 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     5281 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     5341 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     5401 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgatctta
     5461 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     5521 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5581 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5641 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     5701 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5761 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     5821 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     5881 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     5941 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6001 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     6061 accgtcgacc aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     6121 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     6181 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6241 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6301 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa
     6361 ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg
     6421 tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc
     6481 ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga
     6541 cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc
     6601 agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc
     6661 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
     6721 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
     6781 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
     6841 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
     6901 atggtgcact ctcagtacaa tctagctctg atgccgcata gttaagccag ccccgacacc
     6961 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     7021 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     7081 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     7141 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     7201 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     7261 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     7321 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     7381 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     7441 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     7501 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     7561 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     7621 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7681 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7741 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7801 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     7861 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     7921 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7981 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     8041 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     8101 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     8161 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     8221 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     8281 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8341 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8401 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8461 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8521 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8581 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8641 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8701 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8761 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8821 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8881 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8941 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     9001 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     9061 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     9121 gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     9181 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     9241 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     9301 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     9361 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     9421 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     9481 aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt
     9541 cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc
     9601 cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata
     9661 aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga
     9721 tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg
     9781 ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca
     9841 tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct
     9901 gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg
     9961 tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc
    10021 aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg
    10081 gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc
    10141 ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt
    10201 ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt
    10261 tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg
    10321 cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga
    10381 ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg
    10441 cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg
    10501 gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac
    10561 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
    10621 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
    10681 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
    10741 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
    10801 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
    10861 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
    10921 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
    10981 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
    11041 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
    11101 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
    11161 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
    11221 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
    11281 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    11341 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
    11401 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
    11461 cctagtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    11521 attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa
    11581 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
    11641 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
    11701 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
    11761 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
    11821 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
    11881 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
    11941 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
    12001 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
    12061 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    12121 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    12181 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
    12241 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
    12301 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
    12361 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
    12421 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
    12481 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
    12541 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
    12601 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
    12661 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggtga tcttcagacc
    12721 tggacgatat atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
    12781 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
    12841 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
    12901 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
    12961 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
    13021 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
    13081 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
    13141 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
    13201 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
    13261 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
    13321 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
    13381 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
    13441 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
    13501 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
    13561 ggatctcgac ggtcg
//
