LOCUS       V033765                13761 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033765
VERSION     V033765
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..3217
                     /label="TRIM71(NM_001039111)"
                     /note="TRIM71(NM_001039111)"
                     /gene="TRIM71"
     CDS             3218..3244
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3290..3631
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3757..4256
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4277..4984
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4985..5041
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             5042..5641
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5652..6240
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6303..6536
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6561..6579)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7368..7472
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7473..8333
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8504..9092
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9338..9667
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10801..10866
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10996..11016
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11441..11575
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11744..12377
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12424..12549
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    13041..13274
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13459..13503
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctcagtca ttagcagtga
      601 actccaagcc accatggctt cgttccccga gaccgatttc cagatctgct tgctgtgcaa
      661 ggagatgtgc ggctcgccgg cgccgctctc ctccaactcg tccgcgtcgt cgtcctcctc
      721 gcagacgtcc acgtcgtcgg ggggcggcgg cgggggccct ggggcggcgg cgcgccgcct
      781 acacgtcctg ccctgcctgc acgccttctg ccgcccctgc ctcgaggcgc accggctgcc
      841 ggcggcgggc ggcggcgcgg cgggagagcc gctcaagctg cgctgccccg tgtgcgacca
      901 gaaagtagtg ctagccgagg cggcgggtat ggacgcgctg ccttcgtccg ccttcctgct
      961 tagcaacctg ctcgacgcgg tggtggccac tgccgacgag ccgccgccca agaacgggcg
     1021 cgccggcgct ccggcgggag cgggcggcca cagcaaccac cggcaccacg ctcaccacgc
     1081 gcacccgcgc gcgtccgcct ccgcgccgcc actcccgcag gcgccgcagc cgcccgcgcc
     1141 ttcccgctcg gcacccggcg gccctgccgc ttccccgtcg gcgctgctgc tccgccgtcc
     1201 tcacggctgc agctcgtgcg atgagggcaa cgcagcttct tcgcgctgcc tcgactgcca
     1261 ggagcacctg tgcgacaact gcgtccgagc gcaccagcgc gtgcgcctca ccaaggacca
     1321 ctacatcgag cgcggcccgc cgggtcccgg tgccgcagca gcggcgcagc agctcgggct
     1381 cgggccgccc tttcccggcc cgcccttctc catcctctca gtgtttcccg agcgcctcgg
     1441 cttctgccag caccacgacg acgaggtgct gcacctgtac tgtgacactt gctctgtacc
     1501 catctgtcgt gagtgcacaa tgggccggca tgggggccac agcttcatct acctccagga
     1561 ggcactgcag gactcacggg cactcaccat ccagctgctg gcagatgccc agcagggacg
     1621 acaggcaatc cagctgagca tcgagcaggc ccagacggtg gcggaacagg tggagatgaa
     1681 ggcgaaggtt gtgcagtcgg aggtcaaagc cgtgacggcg aggcataaga aagccctgga
     1741 ggaacgcgag tgtgagctgc tgtggaaggt agaaaagatc cgccaggtga aagccaagtc
     1801 tctgtacctg caggtggaga agctgcggca aaacctcaac aagcttgaga gcaccatcag
     1861 tgccgtgcag caggtcctgg aggagggtag agcgctagac atcctactgg cccgagaccg
     1921 gatgctggcc caggtgcagg agctgaagac cgtgcggagc ctcctgcagc cccaggaaga
     1981 cgaccgagtc atgttcacac cccccgatca ggcactgtac cttgccatca agtcttttgg
     2041 ctttgttagc agcggggcct ttgccccact caccaaggcc acaggcgatg gcctcaagcg
     2101 tgccctccag ggtaaggtgg cctccttcac agtcattggt tatgaccacg atggtgagcc
     2161 ccgcctctca ggaggcgacc tgatgtcggc tgtggtcctg ggccctgatg gcaacctgtt
     2221 tggtgcagag gtgagtgatc agcagaatgg gacatacgtg gtgagttacc gaccccagct
     2281 ggagggtgag cacctggtat ctgtgacact gtgcaaccag cacattgaga acagcccttt
     2341 caaggtggtg gtcaagtcag gccgcagcta cgtgggcatt gggctcccgg gcctgagctt
     2401 cggcagtgag ggtgacagcg atggcaagct ctgccgccct tggggtgtga gtgtagacaa
     2461 ggagggctac atcattgtcg ccgaccgcag caacaaccgc atccaggtgt tcaagccctg
     2521 cggcgccttc caccacaaat tcggcaccct gggctcccgg cctgggcagt tcgaccgacc
     2581 agccggcgtg gcctgtgacg cctcacgcag gatcgtggtg gctgacaagg acaatcatcg
     2641 catccagatc ttcacgttcg agggccagtt cctcctcaag tttggtgaga aaggaaccaa
     2701 gaatgggcag ttcaactacc cttgggatgt ggcggtgaat tctgagggca agatcctggt
     2761 ctcagacacg aggaaccacc ggatccagct gtttgggcct gatggtgtct tcctaaacaa
     2821 gtatggcttc gagggggctc tctggaagca ctttgactcc ccacggggtg tggccttcaa
     2881 ccatgagggc cacttggtgg tcactgactt caacaaccac cggctcctgg ttattcaccc
     2941 cgactgccag tcggcacgct ttctgggctc ggagggcaca ggcaatgggc agttcctgcg
     3001 cccacaaggg gtagctgtgg accaggaagg gcgcatcatt gtggcggatt ccaggaacca
     3061 tcgggtacag atgtttgaat ccaacggcag cttcctgtgc aagtttggtg ctcaaggcag
     3121 cggctttggg cagatggacc gcccttccgg catcgccatc acccccgacg gaatgatcgt
     3181 tgtggtggac tttggcaaca atcgaatcct cgtcttctac ccctacgacg tgcctgacta
     3241 cgccggcggc ggtggttcag gcggtggcgg ttccggtggt gggggatcag tccaactggt
     3301 ggagtctggt ggcgctttgg tgcagccagg tggctctctg cgtttgtcct gtgccgcttc
     3361 tggcttccca gtgaaccgct attccatgcg ctggtatcgc caggctccag gcaaagagcg
     3421 tgagtgggta gccggtatgt ccagcgcggg tgatcgtagc tcctatgaag actccgtgaa
     3481 gggccgtttc accatcagcc gtgacgatgc ccgtaacacg gtgtatctgc aaatgaacag
     3541 cttgaaacct gaagatacgg ccgtgtatta ctgtaatgtg aacgtgggct tcgagtattg
     3601 gggccaaggc acccaggtca ccgtctccag ctaagatcct tgacttgcgg ccgcaactcc
     3661 cacctgcaac atgcgtgact gactgaggcc gcgactctag agtcgacctg catctaggcg
     3721 ccggaattag atctctcgag gttaacgaat tctaccgggt aggggaggcg cttttcccaa
     3781 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
     3841 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
     3901 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
     3961 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
     4021 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
     4081 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
     4141 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
     4201 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
     4261 gcagcccaag cttaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     4321 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     4381 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     4441 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     4501 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     4561 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     4621 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     4681 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     4741 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     4801 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     4861 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     4921 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     4981 caaggcaaca aacttctctc tgctgaaaca agccggagat gtcgaagaga atcctggacc
     5041 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     5101 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     5161 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     5221 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     5281 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     5341 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     5401 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     5461 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     5521 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     5581 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     5641 atcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5701 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5761 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5821 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     5881 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     5941 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6001 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     6061 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6121 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6181 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6241 atcgataccg tcgaccaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6301 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     6361 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6421 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6481 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     6541 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
     6601 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
     6661 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
     6721 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
     6781 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     6841 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     6901 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     6961 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     7021 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     7081 cgtcgaatgg tgcactctca gtacaatcta gctctgatgc cgcatagtta agccagcccc
     7141 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     7201 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     7261 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     7321 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7381 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7441 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7501 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7561 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7621 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7681 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7741 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7801 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7861 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7921 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7981 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     8041 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     8101 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     8161 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     8221 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8281 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8341 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8401 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8461 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8521 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8581 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8641 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8701 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8761 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8821 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8881 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8941 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     9001 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     9061 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     9121 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     9181 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     9241 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     9301 ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa
     9361 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     9421 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     9481 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     9541 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9601 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     9661 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc
     9721 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
     9781 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
     9841 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
     9901 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
     9961 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat
    10021 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
    10081 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
    10141 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
    10201 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
    10261 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
    10321 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
    10381 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
    10441 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
    10501 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc
    10561 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg
    10621 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg
    10681 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa
    10741 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
    10801 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
    10861 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
    10921 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
    10981 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
    11041 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
    11101 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
    11161 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
    11221 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
    11281 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
    11341 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
    11401 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
    11461 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    11521 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
    11581 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
    11641 caattaccta gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    11701 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac
    11761 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
    11821 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
    11881 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
    11941 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
    12001 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
    12061 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
    12121 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
    12181 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
    12241 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
    12301 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
    12361 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
    12421 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
    12481 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
    12541 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
    12601 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
    12661 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
    12721 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
    12781 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
    12841 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggtgatctt
    12901 cagacctgga cgatatatat gagggacaat tggagaagtg aattatataa atataaagta
    12961 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
    13021 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
    13081 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
    13141 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
    13201 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
    13261 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
    13321 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
    13381 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
    13441 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
    13501 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
    13561 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
    13621 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
    13681 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
    13741 gtgaacggat ctcgacggtc g
//
