LOCUS       V033509                12357 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033509
VERSION     V033509
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..1813
                     /label="TRIM54(NM_032546)"
                     /note="TRIM54(NM_032546)"
                     /gene="TRIM54"
     CDS             1814..1840
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1886..2227
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2353..2852
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             2873..3580
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3581..3637
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3638..4237
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4248..4836
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             4899..5132
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5157..5175)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        5964..6068
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6069..6929
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7100..7688
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        7934..8263
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9397..9462
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9592..9612
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10037..10171
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10340..10973
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11020..11145
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11637..11870
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12055..12099
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttcttttata atagcagtga
      601 actccaagcc accatgaact tcacagtggg tttcaagccg ctgctagggg atgcacacag
      661 catggacaac ctggagaagc agctcatctg ccccatctgc ctggagatgt tctccaaacc
      721 agtggtgatc ctgccctgcc aacacaacct gtgccgcaaa tgtgccaacg acgtcttcca
      781 ggcctcgaat cctctatggc agtcccgggg ctccaccact gtgtcttcag gaggccgttt
      841 ccgctgccca tcgtgcaggc atgaggttgt cctggacaga cacggtgtct acggcctgca
      901 gcgaaacctg ctagtggaga acattatcga catttacaag caggagtcat ccaggccgct
      961 gcactccaag gctgagcagc acctcatgtg cgaggagcat gaagaagaga agatcaatat
     1021 ttactgcctg agctgtgagg tgcccacctg ctctctctgc aaggtcttcg gtgcccacaa
     1081 ggactgtgag gtggccccac tgcccaccat ttacaaacgc cagaagaaac aggatctcac
     1141 tctgttgccc aggctggagt gcagtggcac aaacacaact tactgcagcc ttgatctccc
     1201 gagctcaagt gatcctccca tcttagcctc gcagaacact aagattatag atagtgagct
     1261 cagcgatggc atcgcgatgc tggtggcagg caatgaccgc gtgcaagcag tgatcacaca
     1321 gatggaggag gtgtgccaga ctatcgagga caatagccgg aggcagaagc agttgttaaa
     1381 ccagaggttt gagagcctgt gcgcagtgct ggaggagcgc aagggtgagc tgctgcaggc
     1441 gctggcccgg gagcaagagg agaagctgca gcgcgtccgc ggcctcatcc gtcagtatgg
     1501 cgaccacctg gaggcctcct ctaagctggt ggagtctgcc atccagtcca tggaagagcc
     1561 acaaatggcg ctgtatctcc agcaggccaa ggagctgatc aataaggtcg gggccatgtc
     1621 gaaggtggag ctggcagggc ggccggagcc aggctatgag agcatggagc aattcaccgt
     1681 aagggtggag cacgtggccg aaatgctgcg gaccatcgac ttccagccag gcgcttccgg
     1741 ggaggaagag gaggtggccc cagacggaga ggagggcagc gcggggccgg aggaagagcg
     1801 gccggatggg ccttacccct acgacgtgcc tgactacgcc ggcggcggtg gttcaggcgg
     1861 tggcggttcc ggtggtgggg gatcagtcca actggtggag tctggtggcg ctttggtgca
     1921 gccaggtggc tctctgcgtt tgtcctgtgc cgcttctggc ttcccagtga accgctattc
     1981 catgcgctgg tatcgccagg ctccaggcaa agagcgtgag tgggtagccg gtatgtccag
     2041 cgcgggtgat cgtagctcct atgaagactc cgtgaagggc cgtttcacca tcagccgtga
     2101 cgatgcccgt aacacggtgt atctgcaaat gaacagcttg aaacctgaag atacggccgt
     2161 gtattactgt aatgtgaacg tgggcttcga gtattggggc caaggcaccc aggtcaccgt
     2221 ctccagctaa gatccttgac ttgcggccgc aactcccacc tgcaacatgc gtgactgact
     2281 gaggccgcga ctctagagtc gacctgcatc taggcgccgg aattagatct ctcgaggtta
     2341 acgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     2401 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     2461 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     2521 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     2581 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     2641 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     2701 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     2761 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     2821 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatggtgag
     2881 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     2941 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     3001 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     3061 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     3121 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     3181 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     3241 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     3301 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     3361 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     3421 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     3481 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     3541 gggccgccac tccaccggcg gcatggacga gctgtacaag gcaacaaact tctctctgct
     3601 gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg accgagtaca agcccacggt
     3661 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     3721 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     3781 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     3841 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     3901 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     3961 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     4021 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     4081 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     4141 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     4201 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgatct tatcgataat caacctctgg
     4261 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     4321 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     4381 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     4441 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     4501 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     4561 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     4621 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     4681 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     4741 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     4801 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga ccaaggcagc
     4861 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     4921 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     4981 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     5041 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     5101 acccttttag tcagtgtgga aaatctctag cagcatctag aattaattcc gtgtattcta
     5161 tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt
     5221 tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta
     5281 tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct
     5341 ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc
     5401 cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
     5461 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
     5521 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
     5581 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
     5641 ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac
     5701 aatctagctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     5761 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     5821 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     5881 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     5941 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6001 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6061 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6121 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6181 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6241 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6301 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6361 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6421 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6481 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6541 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6601 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6661 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6721 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6781 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6841 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6901 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6961 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7021 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7081 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7141 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7201 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     7261 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7321 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7381 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7441 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7501 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7561 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7621 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7681 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7741 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7801 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7861 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     7921 gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     7981 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     8041 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     8101 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8161 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     8221 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac
     8281 aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
     8341 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg
     8401 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
     8461 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
     8521 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
     8581 tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat
     8641 ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga
     8701 tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat
     8761 tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc
     8821 gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga
     8881 tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact
     8941 gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg
     9001 aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac
     9061 atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt
     9121 cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga
     9181 tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg
     9241 atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact
     9301 ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa
     9361 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
     9421 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
     9481 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
     9541 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
     9601 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
     9661 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
     9721 gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac
     9781 agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt
     9841 aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag
     9901 gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga
     9961 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
    10021 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    10081 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    10141 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
    10201 tcccaaactt cccaccccat accctattac cactgccaat tacctagtgg tttcatttac
    10261 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
    10321 gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt
    10381 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
    10441 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
    10501 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
    10561 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
    10621 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
    10681 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
    10741 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
    10801 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    10861 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    10921 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
    10981 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
    11041 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
    11101 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
    11161 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
    11221 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
    11281 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
    11341 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
    11401 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
    11461 agtaagacca ccgcacagca agcggccggt gatcttcaga cctggacgat atatatgagg
    11521 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
    11581 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
    11641 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
    11701 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
    11761 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
    11821 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
    11881 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
    11941 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
    12001 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
    12061 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
    12121 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
    12181 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
    12241 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
    12301 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtcg
//
