LOCUS       V033214                13434 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033214
VERSION     V033214
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2890
                     /label="TRIM46(NM_025058)"
                     /note="TRIM46(NM_025058)"
                     /gene="TRIM46"
     CDS             2891..2917
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2963..3304
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3430..3929
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3950..4657
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4658..4714
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4715..5314
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5325..5913
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5976..6209
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6234..6252)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7041..7145
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7146..8006
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8177..8765
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9011..9340
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10474..10539
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10669..10689
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11114..11248
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11417..12050
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12097..12222
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12714..12947
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13132..13176
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctcaccgg taggcagtga
      601 actccaagcc accatggcag agggtgagga tatgcagacc ttcacttcca tcatggacgc
      661 actggtccgc atcagtacca gcatgaagaa catggagaag gaactgctgt gcccagtgtg
      721 tcaagagatg tacaagcagc cactggtgct gccctgtacc cacaacgtgt gccaggcctg
      781 tgcccgagag gtcttgggcc agcagggcta cataggacat ggtggggacc ccagctccga
      841 gcccacctct cctgcctcca ccccttccac ccgcagcccc cgcctctccc gcagaactct
      901 ccccaagcca gaccgcctgg accggctgct taagtcaggc tttgggacat accctgggag
      961 gaagcgaggt gctttgcacc cccaagtgat catgttcccg tgcccagcct gccaaggtga
     1021 tgtggagctt ggggagcggg gtctggcagg gcttttccgg aacctgaccc tggagcgtgt
     1081 ggtggagcgg taccgccaga gtgtgagtgt gggaggtgcc atcctgtgcc agttgtgcaa
     1141 gcccccacca ctagaggcca ccaagggctg cacagagtgc cgcgccacct tctgcaatga
     1201 gtgcttcaag ctcttccacc cctggggcac ccagaaggcc cagcatgagc ccaccctgcc
     1261 taccctctcc ttccgaccca agggccttat gtgcccagac cacaaggaag aggtgaccca
     1321 ctactgcaag acatgccaac gcctggtatg tcaactctgc cgggtgcggc gcacccacag
     1381 cgggcacaag atcacaccag tgctcagtgc ctaccaggcc ctcaaggaca agctgacaaa
     1441 gagcctgaca tacatcctgg gaaaccagga cacggtacag acccagatct gtgagctgga
     1501 ggaggccgtg aggcacaccg aggtgagtgg tcagcaggcc aaggaggagg tgtcgcagct
     1561 ggtgcggggg ctgggggctg tgctggagga gaagcgggca tcactgcttc aggccattga
     1621 agaatgccag caggagcggc tggcccgtct cagcgcccag atccaggagc accggagcct
     1681 gctggatggc tcaggtctgg tgggctatgc ccaggaagta cttaaggaaa cagaccagcc
     1741 ttgctttgtg caagccgcca agcagctgca caacaggatt gcccgagcca ctgaagccct
     1801 ccagacattc cggccagctg ccagctcctc cttccgccat tgccagctcg acgtgggacg
     1861 tgagatgaag ctgctgacag agcttaactt cctgcgagtg cctgaggccc ccgtcattga
     1921 cacccagcgc acctttgcct atgatcagat cttcctgtgc tggcggctgc ccccccattc
     1981 accacctgcc tggcactata ccgttgagtt ccggcgcacg gatgtgcctg ctcagccagg
     2041 ccccacccgc tggcagcggc gggaggaggt gaggggcacc agtgccctgc ttgagaaccc
     2101 cgacacgggc tctgtgtatg tgctgcgtgt ccgcggctgc aacaaggccg gctacggcga
     2161 atacagtgaa gatgtgcacc tgcacacgcc cccggcacct gtcctgcact tcttcctcga
     2221 tagccgctgg ggcgcaagcc gagagcggct ggctatcagc aaggaccagc gagcagtacg
     2281 gagtgttcca gggctgcccc tgctgctggc tgctgaccgg ctgctgaccg gctgccacct
     2341 gagtgtggat gtggtcctgg gcgacgtggc tgtgacccag ggccgcagct actgggcctg
     2401 cgccgtagac ccagcctcct acttggtcaa ggtgggcgtc gggctggaga gcaagcttca
     2461 agaaagtttc cagggtgccc ccgatgtgat cagccccagg tacgacccgg acagcgggca
     2521 cgacagcggt gccgaggatg ccacagtgga ggcgtcgcca cccttcgctt tcctaaccat
     2581 tggcatgggc aagatcctgc tggggtcggg ggcaagctca aacgcagggc tgacagggag
     2641 ggatggcccc acagccggct gcacagtgcc cctgccaccc cgcctgggca tctgcctgga
     2701 ctatgagcgg ggccgggttt ccttcctgga tgctgtttcc ttccgtgggc tcttggagtg
     2761 ccccctggac tgctcagggc ctgtgtgccc tgccttttgc ttcatcgggg gtggcgcagt
     2821 acagctccag gagccagtgg gcactaagcc tgagaggaaa gtcaccattg ggggcttcgc
     2881 caagctggac tacccctacg acgtgcctga ctacgccggc ggcggtggtt caggcggtgg
     2941 cggttccggt ggtgggggat cagtccaact ggtggagtct ggtggcgctt tggtgcagcc
     3001 aggtggctct ctgcgtttgt cctgtgccgc ttctggcttc ccagtgaacc gctattccat
     3061 gcgctggtat cgccaggctc caggcaaaga gcgtgagtgg gtagccggta tgtccagcgc
     3121 gggtgatcgt agctcctatg aagactccgt gaagggccgt ttcaccatca gccgtgacga
     3181 tgcccgtaac acggtgtatc tgcaaatgaa cagcttgaaa cctgaagata cggccgtgta
     3241 ttactgtaat gtgaacgtgg gcttcgagta ttggggccaa ggcacccagg tcaccgtctc
     3301 cagctaagat ccttgacttg cggccgcaac tcccacctgc aacatgcgtg actgactgag
     3361 gccgcgactc tagagtcgac ctgcatctag gcgccggaat tagatctctc gaggttaacg
     3421 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     3481 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     3541 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     3601 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     3661 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     3721 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     3781 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     3841 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     3901 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca tggtgagcaa
     3961 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     4021 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     4081 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     4141 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     4201 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     4261 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     4321 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     4381 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     4441 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     4501 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     4561 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     4621 ccgccactcc accggcggca tggacgagct gtacaaggca acaaacttct ctctgctgaa
     4681 acaagccgga gatgtcgaag agaatcctgg accgatgacc gagtacaagc ccacggtgcg
     4741 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     4801 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     4861 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     4921 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     4981 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     5041 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     5101 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     5161 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     5221 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     5281 cacctggtgc atgacccgca agcccggtgc ctgatcttat cgataatcaa cctctggatt
     5341 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     5401 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     5461 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     5521 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     5581 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     5641 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     5701 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     5761 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     5821 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     5881 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacca aggcagctgt
     5941 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     6001 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     6061 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6121 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6181 cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag
     6241 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
     6301 aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca
     6361 tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt
     6421 aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag
     6481 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
     6541 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
     6601 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
     6661 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
     6721 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
     6781 ctagctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     6841 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     6901 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     6961 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     7021 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     7081 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     7141 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     7201 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     7261 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     7321 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     7381 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7441 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7501 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7561 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7621 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7681 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7741 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     7801 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7861 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7921 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7981 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     8041 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     8101 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8161 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8221 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8281 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     8341 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8401 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8461 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8521 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8581 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8641 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8701 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8761 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8821 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     8881 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8941 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     9001 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     9061 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     9121 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     9181 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     9241 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     9301 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg
     9361 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
     9421 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
     9481 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
     9541 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
     9601 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt
     9661 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
     9721 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
     9781 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
     9841 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
     9901 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
     9961 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
    10021 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
    10081 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
    10141 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
    10201 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
    10261 ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
    10321 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga
    10381 tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
    10441 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
    10501 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
    10561 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
    10621 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
    10681 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
    10741 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
    10801 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
    10861 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
    10921 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
    10981 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
    11041 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
    11101 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    11161 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    11221 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
    11281 caaacttccc accccatacc ctattaccac tgccaattac ctagtggttt catttactct
    11341 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
    11401 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
    11461 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
    11521 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
    11581 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
    11641 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
    11701 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
    11761 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
    11821 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
    11881 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
    11941 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    12001 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
    12061 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
    12121 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
    12181 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
    12241 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
    12301 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
    12361 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
    12421 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
    12481 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
    12541 aagaccaccg cacagcaagc ggccggtgat cttcagacct ggacgatata tatgagggac
    12601 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
    12661 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
    12721 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
    12781 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
    12841 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
    12901 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
    12961 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
    13021 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
    13081 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
    13141 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
    13201 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
    13261 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
    13321 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
    13381 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtcg
//
