LOCUS       V030050                13326 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030050
VERSION     V030050
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2782
                     /label="TRIM42(NM_152616)"
                     /note="TRIM42(NM_152616)"
                     /gene="TRIM42"
     CDS             2783..2809
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2855..3196
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3322..3821
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3842..4549
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4550..4606
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4607..5206
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5217..5805
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5868..6101
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6126..6144)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6933..7037
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7038..7898
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8069..8657
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8903..9232
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10366..10431
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10561..10581
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11006..11140
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11309..11942
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11989..12114
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12606..12839
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13024..13068
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctcctcga taggcagtga
      601 actccaagcc accatggaaa ctgctatgtg cgtttgctgt ccatgttgta catggcagag
      661 atgttgtcct cagttatgct cctgtctgtg ctgcaagttc atcttcacct cagagcggaa
      721 ctgcacctgc ttcccctgcc cttacaaaga tgagcggaac tgccagttct gccactgcac
      781 ctgttctgag agccccaact gccattggtg ttgctgctct tgggccaatg atcccaactg
      841 taagtgctgc tgcacagcca gcagcaatct caactgctac tactatgaga gccgctgctg
      901 ccgcaatacc atcatcactt tccacaaggg ccgcctcagg agcatccata cctcctccaa
      961 gactgccctg cgcactggga gcagcgatac ccaggtggat gaagtaaagt caataccagc
     1021 caacagtcac ctggtgaacc acctcaattg ccccatgtgc agccggctgc gcctgcactc
     1081 attcatgctg ccctgcaacc acagcctgtg cgagaagtgc ctgcggcagc tgcagaagca
     1141 cgccgaggtc accgagaact tcttcatcct catctgccca gtgtgcgacc gctcgcactg
     1201 catgccctac agcaacaaga tgcagctgcc cgagaactac ctgcacgggc gtctcaccaa
     1261 gcgctacatg caggagcacg gctacctcaa gtggcgcttt gaccgctcct ccgggcccat
     1321 cctctgccag gtctgccgca acaagcgcat cgcttacaag cgctgcatca cctgccgcct
     1381 caacctgtgc aacgactgcc tcaaggcctt ccactcggat gtggccatgc aagaccacgt
     1441 ctttgtggac accagcgccg aggaacagga cgagaagatc tgcatccacc acccatccag
     1501 ccgcatcatc gagtactgcc gcaatgacaa caaattgctc tgcaccttct gcaagttctc
     1561 tttccacaat ggccacgaca ccattagcct catcgacgcc tgctccgaga gggccgcctc
     1621 actcttcagc gccatcgcca agttcaaagc agtccgatat gaaattgata atgacctaat
     1681 ggaattcaac atcttaaaaa acagctttaa agctgacaag gaggcaaagc gaaaagagat
     1741 cagaaatggc tttctcaagt tgcgcagcat tcttcaggag aaagagaaga tcatcatgga
     1801 gcagatagag aatctagaag tgtccaggca gaaggaaatt gaaaaatatg tgtatgttac
     1861 aaccatgaaa gtgaacgaga tggatggtct gatcgcctac tccaaggaag ccctgaagga
     1921 gactggccag gtggcattcc tgcagtcagc caagatcctg gtggaccaga tcgaggacgg
     1981 catccagacc acctacaggc ctgacccaca gctccggctg cactcaataa actacgtgcc
     2041 cttggacttt gttgagcttt ccagtgccat ccatgagctc ttccccacag ggcccaagaa
     2101 ggtacgctcc tcaggggact ccctgccctc cccctacccc gtgcactcag aaacaatgat
     2161 tgccaggaag gtcactttca gcacccacag cctcggcaac cagcacatat accagcgaag
     2221 ctcctccatg ttgtccttca gcaacactga caagaaggcc aaggtgggtc tggaggcctg
     2281 tgggagagcc cagtcagcca cccccgccaa acccacagac ggcctctaca cctactggag
     2341 tgctggagca gacagccagt ctgtacagaa cagcagcagc ttccacaact ggtactcatt
     2401 caacgatggc tctgtgaaga ccccaggccc aattgttatc taccagactc tggtgtaccc
     2461 aagagctgcc aaggtttact ggacatgtcc agcagaagac gtggactctt ttgagatgga
     2521 attctatgaa gtcattactt ctcctcctaa caacgtacaa atggagctct gtggacaaat
     2581 tcgggacata atgcagcaaa atctggagct gcacaacctg acccccaaca cagaatacgt
     2641 gtttaaagtt agagccatca atgataatgg tcctgggcaa tggagtgata tctgcaaggt
     2701 ggtaacacca gatggacatg ggaagaaccg agctaagtgg ggcctgctga agaatatcca
     2761 gtctgccctc cagaagcact tctaccccta cgacgtgcct gactacgccg gcggcggtgg
     2821 ttcaggcggt ggcggttccg gtggtggggg atcagtccaa ctggtggagt ctggtggcgc
     2881 tttggtgcag ccaggtggct ctctgcgttt gtcctgtgcc gcttctggct tcccagtgaa
     2941 ccgctattcc atgcgctggt atcgccaggc tccaggcaaa gagcgtgagt gggtagccgg
     3001 tatgtccagc gcgggtgatc gtagctccta tgaagactcc gtgaagggcc gtttcaccat
     3061 cagccgtgac gatgcccgta acacggtgta tctgcaaatg aacagcttga aacctgaaga
     3121 tacggccgtg tattactgta atgtgaacgt gggcttcgag tattggggcc aaggcaccca
     3181 ggtcaccgtc tccagctaag atccttgact tgcggccgca actcccacct gcaacatgcg
     3241 tgactgactg aggccgcgac tctagagtcg acctgcatct aggcgccgga attagatctc
     3301 tcgaggttaa cgaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     3361 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     3421 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     3481 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     3541 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     3601 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     3661 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     3721 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     3781 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
     3841 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     3901 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     3961 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     4021 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     4081 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     4141 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     4201 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     4261 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     4321 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     4381 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     4441 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     4501 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg caacaaactt
     4561 ctctctgctg aaacaagccg gagatgtcga agagaatcct ggaccgatga ccgagtacaa
     4621 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     4681 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     4741 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     4801 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     4861 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     4921 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     4981 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     5041 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     5101 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     5161 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgatctt atcgataatc
     5221 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5281 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5341 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5401 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5461 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     5521 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5581 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     5641 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     5701 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     5761 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     5821 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     5881 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     5941 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6001 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6061 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
     6121 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
     6181 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
     6241 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
     6301 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
     6361 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     6421 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     6481 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     6541 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     6601 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
     6661 tctcagtaca atctagctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     6721 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     6781 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     6841 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     6901 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6961 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7021 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7081 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7141 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7201 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7261 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7321 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7381 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7441 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7501 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7561 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7621 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7681 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7741 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7801 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7861 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     7921 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     7981 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8041 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8101 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8161 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     8221 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8281 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8341 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8401 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8461 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8521 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8581 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8641 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8701 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8761 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     8821 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     8881 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     8941 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     9001 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     9061 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9121 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     9181 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
     9241 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
     9301 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
     9361 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
     9421 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
     9481 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
     9541 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     9601 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
     9661 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
     9721 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
     9781 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
     9841 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
     9901 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa
     9961 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
    10021 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
    10081 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
    10141 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg
    10201 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg
    10261 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt
    10321 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
    10381 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
    10441 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
    10501 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
    10561 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
    10621 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
    10681 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
    10741 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
    10801 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
    10861 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
    10921 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
    10981 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
    11041 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    11101 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    11161 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctagtggt
    11221 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    11281 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
    11341 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
    11401 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
    11461 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
    11521 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
    11581 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
    11641 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
    11701 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
    11761 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    11821 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    11881 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    11941 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
    12001 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
    12061 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
    12121 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
    12181 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
    12241 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
    12301 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
    12361 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    12421 caaaacaaaa gtaagaccac cgcacagcaa gcggccggtg atcttcagac ctggacgata
    12481 tatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
    12541 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
    12601 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
    12661 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
    12721 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
    12781 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
    12841 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
    12901 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
    12961 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
    13021 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
    13081 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
    13141 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
    13201 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
    13261 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
    13321 cggtcg
//
