LOCUS       V033392                14538 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033392
VERSION     V033392
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..3994
                     /label="TRIM33(NM_015906)"
                     /note="TRIM33(NM_015906)"
                     /gene="TRIM33"
     CDS             3995..4021
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4067..4408
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        4534..5033
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5054..5761
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5762..5818
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             5819..6418
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6429..7017
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7080..7313
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(7338..7356)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        8145..8249
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             8250..9110
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      9281..9869
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        10115..10444
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          11578..11643
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             11773..11793
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    12218..12352
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             12521..13154
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    13201..13326
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    13818..14051
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             14236..14280
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttcttgtttt aaagcagtga
      601 actccaagcc accatggcgg aaaacaaagg cggcggcgag gctgagagcg gcggcggggg
      661 cagcggcagc gcgccggtaa ctgccggggc cgccgggccc gccgcgcagg aggcggagcc
      721 gcctctcacc gcggtgctgg tggaggagga ggaggaggaa ggcggcaggg ccggcgctga
      781 gggcggcgcg gccgggcccg acgacggggg ggtggccgcg gcctcctcgg gctcggccca
      841 ggctgcttca tctcctgcgg cctcagtggg cactggagtt gccgggggcg cagtatcgac
      901 gccggctcca gctccagcct cggctcccgc tccgggtccc tcggcagggc cgcctcctgg
      961 accgccagcc tcgctcctgg acacctgcgc cgtgtgtcag cagagcttgc agagccggcg
     1021 tgaggcggag cccaagctgc tgccctgtct tcactccttc tgcctgcgct gcctgcccga
     1081 gccggagcgc cagctcagcg tgcccatccc ggggggcagc aacggcgaca tccagcaagt
     1141 tggtgtaata cggtgcccag tatgccgcca agaatgcaga cagatagacc ttgtggataa
     1201 ttattttgtg aaagacacat ctgaagctcc tagcagttct gatgaaaaat cagaacaggt
     1261 atgtactagt tgtgaagaca atgcaagtgc agttggcttt tgtgtagaat gtggagagtg
     1321 gctatgtaag acatgtatcg aagcacatca aagagtaaaa tttactaaag atcacttgat
     1381 caggaagaaa gaagatgtct cagagtctgt tggagcatct ggtcaacgcc ctgttttctg
     1441 ccctgtacac aaacaagaac agttgaaact tttctgtgaa acatgtgata gattgacatg
     1501 tagagactgt cagctattgg aacacaaaga acataggtat cagtttttgg aagaagcttt
     1561 tcaaaatcag aagggtgcaa ttgagaatct actggcgaaa cttcttgaga agaagaatta
     1621 tgttcatttt gcagctactc aggtgcagaa taggataaaa gaagtaaatg agactaacaa
     1681 acgagtagaa caggaaatta aagtggccat tttcaccctt atcaatgaaa ttaataagaa
     1741 aggaaaatct ctcttacaac agctagagaa tgttacaaag gaaagacaga tgaagttact
     1801 acagcagcag aatgacatca caggcctttc ccggcaggtg aagcatgtta tgaacttcac
     1861 aaattgggca attgcaagtg gcagcagcac agcactacta tacagcaagc gactgattac
     1921 tttccagttg cgtcatattt tgaaagcacg gtgtgatcct gtccctgctg ctaatggagc
     1981 aatacgtttc cattgtgatc ccaccttctg ggcaaagaat gtagtcaatt taggtaatct
     2041 agtaatagag agtaaaccag ctcctggtta tactcctaat gttgtagttg ggcaagttcc
     2101 tccagggaca aaccacatta gtaaaacccc tggacagatt aacttagcac agcttcgact
     2161 ccagcacatg caacaacaag tatatgcaca gaaacatcag cagttgcaac agatgaggat
     2221 gcagcaacca ccagcacctg taccaactac aacaacaaca acacaacagc atcctagaca
     2281 agcagcccct cagatgttac aacaacagcc tcctcgattg atcagtgtgc aaacaatgca
     2341 aagaggcaac atgaactgtg gagcttttca agcccatcag atgagactgg ctcagaatgc
     2401 tgccagaata ccagggatac ccaggcacag cggccctcaa tattccatga tgcagccaca
     2461 cctccaaaga caacactcaa acccagggca tgctggaccc tttcccgtag tatcggtaca
     2521 caacaccaca atcaacccaa cgagccctac tacagcaact atggcaaatg caaaccgagg
     2581 tcccaccagc ccatctgtta cagcaataga gctaatcccc tcagttacca atccagaaaa
     2641 ccttccatcg ctgccagata ttccacccat acagttggaa gatgctggct caagtagttt
     2701 agataatcta ctaagtagat acatctcagg cagtcaccta cccccacagc ctacaagcac
     2761 catgaatcct tctccaggtc cctctgccct ttctccggga tcatcaggtt tatccaattc
     2821 tcacacacct gtgagacccc caagtacttc tagtactggc agtcgaggca gctgtgggtc
     2881 atcaggaaga actgctgaga agacaagtct tagtttcaaa tctgatcagg tgaaggtcaa
     2941 gcaagaacct gggactgaag atgaaatatg tagcttttca ggaggtgtaa aacaagaaaa
     3001 aacagaggat ggcaggagga gtgcttgcat gttgagcagt cctgagagta gcttgacacc
     3061 acctctctca accaacctgc atctagaaag tgaattggat gcattggcaa gcctggaaaa
     3121 ccatgtgaaa attgaacctg cagatatgaa tgaaagctgc aaacagtcag ggctcagcag
     3181 ccttgttaat ggaaagtccc caattcgaag cctcatgcac aggtcggcaa ggattggagg
     3241 agatggcaac aataaagatg atgacccaaa tgaagactgg tgtgctgtct gccaaaacgg
     3301 aggagatctc ttgtgctgcg aaaaatgtcc aaaggtcttt catctaactt gtcatgttcc
     3361 aacactactt agctttccaa gtggggactg gatatgcaca ttttgtagag atattggaaa
     3421 gccagaagtt gaatatgatt gtgataattt gcaacatagt aagaagggga aaactgcgca
     3481 ggggttaagc cccgtggacc aaaggaaatg tgaacgtctt ctgctttacc tctattgcca
     3541 tgaattaagt attgaattcc aggagcctgt tcctgcttcg ataccaaact actataaaat
     3601 tataaagaaa ccaatggatt tatccaccgt gaaaaagaag cttcagaaaa aacattccca
     3661 acactaccaa atcccggatg actttgtggc cgatgtccgt ttgatcttca agaactgtga
     3721 aaggtttaat gaaatgatga aagttgttca agtttatgca gacacacaag agattaattt
     3781 gaaggctgat tcagaagtag ctcaggcagg gaaagcagtt gcattgtact ttgaagataa
     3841 actcacagag atctactcag acaggacctt cgcacctttg ccagagtttg agcaggaaga
     3901 ggatgatggt gaggtaactg aggactctga tgaagacttt atacagcccc gcagaaaacg
     3961 cctaaagtca gatgagagac cagtacatat aaagtacccc tacgacgtgc ctgactacgc
     4021 cggcggcggt ggttcaggcg gtggcggttc cggtggtggg ggatcagtcc aactggtgga
     4081 gtctggtggc gctttggtgc agccaggtgg ctctctgcgt ttgtcctgtg ccgcttctgg
     4141 cttcccagtg aaccgctatt ccatgcgctg gtatcgccag gctccaggca aagagcgtga
     4201 gtgggtagcc ggtatgtcca gcgcgggtga tcgtagctcc tatgaagact ccgtgaaggg
     4261 ccgtttcacc atcagccgtg acgatgcccg taacacggtg tatctgcaaa tgaacagctt
     4321 gaaacctgaa gatacggccg tgtattactg taatgtgaac gtgggcttcg agtattgggg
     4381 ccaaggcacc caggtcaccg tctccagcta agatccttga cttgcggccg caactcccac
     4441 ctgcaacatg cgtgactgac tgaggccgcg actctagagt cgacctgcat ctaggcgccg
     4501 gaattagatc tctcgaggtt aacgaattct accgggtagg ggaggcgctt ttcccaaggc
     4561 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     4621 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     4681 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     4741 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     4801 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     4861 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     4921 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     4981 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
     5041 gcccaagctt accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     5101 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     5161 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     5221 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     5281 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     5341 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     5401 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     5461 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     5521 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     5581 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     5641 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     5701 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     5761 ggcaacaaac ttctctctgc tgaaacaagc cggagatgtc gaagagaatc ctggaccgat
     5821 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     5881 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     5941 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     6001 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     6061 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     6121 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     6181 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     6241 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     6301 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     6361 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatc
     6421 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6481 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6541 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6601 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6661 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6721 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6781 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     6841 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6901 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6961 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     7021 gataccgtcg accaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     7081 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     7141 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7201 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7261 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta
     7321 gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt
     7381 atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct
     7441 ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt
     7501 ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca
     7561 atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc
     7621 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     7681 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     7741 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
     7801 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
     7861 cgaatggtgc actctcagta caatctagct ctgatgccgc atagttaagc cagccccgac
     7921 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     7981 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     8041 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     8101 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     8161 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     8221 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     8281 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     8341 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     8401 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     8461 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     8521 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     8581 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     8641 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     8701 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8761 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     8821 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8881 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8941 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     9001 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     9061 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     9121 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     9181 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     9241 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     9301 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     9361 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     9421 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     9481 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     9541 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     9601 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9661 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9721 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9781 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9841 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9901 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9961 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    10021 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    10081 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc
    10141 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
    10201 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
    10261 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
    10321 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    10381 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
    10441 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
    10501 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
    10561 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
    10621 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
    10681 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
    10741 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg
    10801 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
    10861 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
    10921 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
    10981 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
    11041 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
    11101 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
    11161 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
    11221 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
    11281 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca
    11341 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc
    11401 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga
    11461 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct
    11521 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
    11581 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
    11641 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
    11701 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
    11761 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
    11821 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
    11881 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
    11941 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
    12001 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
    12061 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
    12121 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
    12181 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
    12241 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    12301 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
    12361 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
    12421 ttacctagtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    12481 gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc
    12541 caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
    12601 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
    12661 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
    12721 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
    12781 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
    12841 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
    12901 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
    12961 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
    13021 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
    13081 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
    13141 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
    13201 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
    13261 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
    13321 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
    13381 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
    13441 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
    13501 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
    13561 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
    13621 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg tgatcttcag
    13681 acctggacga tatatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
    13741 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
    13801 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
    13861 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
    13921 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
    13981 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
    14041 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
    14101 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
    14161 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
    14221 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
    14281 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
    14341 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
    14401 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
    14461 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
    14521 aacggatctc gacggtcg
//
