LOCUS       V030088                13662 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030088
VERSION     V030088
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..3118
                     /label="TRIM28(NM_005762)"
                     /note="TRIM28(NM_005762)"
                     /gene="TRIM28"
     CDS             3119..3145
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3191..3532
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3658..4157
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4178..4885
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4886..4942
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4943..5542
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5553..6141
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6204..6437
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6462..6480)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7269..7373
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7374..8234
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8405..8993
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9239..9568
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10702..10767
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10897..10917
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11342..11476
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11645..12278
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12325..12450
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12942..13175
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13360..13404
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctgcgacc gaggcagtga
      601 actccaagcc accatggcgg cctccgcggc ggcagcctcg gcagcagcgg cctcggccgc
      661 ctctggcagc ccgggcccgg gcgagggctc cgctggcggc gaaaagcgct ccaccgcccc
      721 ttcggccgca gcctcggcct ctgcctcagc cgcggcgtcg tcgcccgcgg ggggcggcgc
      781 cgaggcgctg gagctgctgg agcactgcgg cgtgtgcaga gagcgcctgc gacccgagag
      841 ggagccccgc ctgctgccct gtttgcactc ggcctgtagt gcctgcttag ggcccgcggc
      901 ccccgccgcc gccaacagct cgggggacgg cggggcggcg ggcgacggca ccgtggtgga
      961 ctgtcccgtg tgcaagcaac agtgcttctc caaagacatc gtggagaatt atttcatgcg
     1021 tgatagtggc agcaaggctg ccaccgacgc ccaggatgcg aaccagtgct gcactagctg
     1081 tgaggataat gccccagcca ccagctactg tgtggagtgc tcggagcctc tgtgtgagac
     1141 ctgtgtagag gcgcaccagc gggtgaagta caccaaggac catactgtgc gctctactgg
     1201 gccagccaag tctcgggatg gtgaacgtac tgtctattgc aacgtacaca agcatgaacc
     1261 ccttgtgctg ttttgtgaga gctgtgatac tctcacctgc cgagactgcc agctcaatgc
     1321 ccacaaggac caccagtacc agttcttaga ggatgcagtg aggaaccagc gcaagctcct
     1381 ggcctcactg gtgaagcgcc ttggggacaa acatgcaaca ttgcagaaga gcaccaagga
     1441 ggttcgcagc tcaatccgcc aggtgtctga cgtacagaag cgtgtgcaag tggatgtcaa
     1501 gatggccatc ctgcagatca tgaaggagct gaataagcgg ggccgtgtgc tggtcaatga
     1561 tgcccagaag gtgactgagg ggcagcagga gcgcctggag cggcagcact ggaccatgac
     1621 caagatccag aagcaccagg agcacattct gcgctttgcc tcttgggctc tggagagtga
     1681 caacaacaca gcccttttgc tttctaagaa gttgatctac ttccagctgc accgggccct
     1741 caagatgatt gtggatcccg tggagccaca tggcgagatg aagtttcagt gggacctcaa
     1801 tgcctggacc aagagtgccg aggcctttgg caagattgtg gcagagcgtc ctggcactaa
     1861 ctcaacaggc cctgcaccca tggcccctcc aagagcccca gggcccctga gcaagcaggg
     1921 ctctggcagc agccagccca tggaggtgca ggaaggctat ggctttgggt caggagatga
     1981 tccctactca agtgcagagc cccatgtgtc aggtgtgaaa cggtcccgct caggtgaggg
     2041 cgaggtgagc ggccttatgc gcaaggtgcc acgagtgagc cttgaacgcc tggacctgga
     2101 cctcacagct gacagccagc cacccgtctt caaggtcttc ccaggcagta ccactgagga
     2161 ctacaacctt attgttattg aacgtggcgc tgccgctgca gctaccggcc agccagggac
     2221 tgcgcctgca ggaacccctg gtgccccacc cctggctggc atggccattg tcaaggagga
     2281 ggagacggag gctgccattg gagcccctcc tactgccact gagggccctg agaccaaacc
     2341 tgtgcttatg gctcttgcgg agggtcctgg tgctgagggt ccccgcctgg cctcacctag
     2401 tggcagcacc agctcagggc tggaggtggt ggctcctgag ggtacctcag ccccaggtgg
     2461 tggcccggga accctggatg acagtgccac catttgccgt gtctgccaga agccaggcga
     2521 tctggttatg tgcaaccagt gtgagttttg tttccacctg gactgtcacc tgccggccct
     2581 gcaggatgta ccaggggagg agtggagctg ctcactctgc catgtgctcc ctgacctgaa
     2641 ggaggaggat ggcagcctca gcctggatgg tgcagacagc actggcgtgg tggccaagct
     2701 ctcaccagcc aaccagcgga aatgtgagcg tgtactgctg gccctattct gtcacgaacc
     2761 ctgccgcccc ctgcatcagc tggctaccga ctccaccttc tccctggacc agcccggtgg
     2821 caccctggat ctgaccctga tccgtgcccg cctccaggag aagttgtcac ctccctacag
     2881 ctccccacag gagtttgccc aggatgtggg ccgcatgttc aagcaattca acaagttaac
     2941 tgaggacaag gcagacgtgc agtccatcat cggcctgcag cgcttcttcg agacgcgcat
     3001 gaacgaggcc ttcggtgaca ccaagttctc tgctgtgctg gtggagcccc cgccgatgag
     3061 cctgcctggt gctggcctga gttcccagga gctgtctggt ggccctggtg atggccccta
     3121 cccctacgac gtgcctgact acgccggcgg cggtggttca ggcggtggcg gttccggtgg
     3181 tgggggatca gtccaactgg tggagtctgg tggcgctttg gtgcagccag gtggctctct
     3241 gcgtttgtcc tgtgccgctt ctggcttccc agtgaaccgc tattccatgc gctggtatcg
     3301 ccaggctcca ggcaaagagc gtgagtgggt agccggtatg tccagcgcgg gtgatcgtag
     3361 ctcctatgaa gactccgtga agggccgttt caccatcagc cgtgacgatg cccgtaacac
     3421 ggtgtatctg caaatgaaca gcttgaaacc tgaagatacg gccgtgtatt actgtaatgt
     3481 gaacgtgggc ttcgagtatt ggggccaagg cacccaggtc accgtctcca gctaagatcc
     3541 ttgacttgcg gccgcaactc ccacctgcaa catgcgtgac tgactgaggc cgcgactcta
     3601 gagtcgacct gcatctaggc gccggaatta gatctctcga ggttaacgaa ttctaccggg
     3661 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     3721 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     3781 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     3841 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     3901 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     3961 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     4021 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     4081 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     4141 catctccggg cctttcgacc tgcagcccaa gcttaccatg gtgagcaagg gcgaggagga
     4201 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     4261 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     4321 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     4381 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     4441 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     4501 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     4561 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     4621 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     4681 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     4741 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     4801 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     4861 cggcggcatg gacgagctgt acaaggcaac aaacttctct ctgctgaaac aagccggaga
     4921 tgtcgaagag aatcctggac cgatgaccga gtacaagccc acggtgcgcc tcgccacccg
     4981 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     5041 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     5101 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     5161 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     5221 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     5281 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     5341 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     5401 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     5461 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     5521 gacccgcaag cccggtgcct gatcttatcg ataatcaacc tctggattac aaaatttgtg
     5581 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     5641 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     5701 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     5761 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     5821 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     5881 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     5941 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     6001 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     6061 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     6121 ccctttgggc cgcctccccg catcgatacc gtcgaccaag gcagctgtag atcttagcca
     6181 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     6241 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6301 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6361 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6421 gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat
     6481 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
     6541 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
     6601 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
     6661 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
     6721 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     6781 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     6841 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     6901 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     6961 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct agctctgatg
     7021 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     7081 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     7141 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     7201 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     7261 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7321 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7381 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7441 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7501 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7561 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7621 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7681 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7741 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7801 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7861 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7921 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7981 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8041 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8101 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8161 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8221 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8281 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8341 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8401 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8461 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8521 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     8581 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8641 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8701 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8761 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8821 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8881 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8941 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9001 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     9061 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     9121 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     9181 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
     9241 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     9301 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     9361 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     9421 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     9481 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     9541 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
     9601 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     9661 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     9721 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     9781 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     9841 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
     9901 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     9961 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
    10021 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
    10081 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
    10141 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
    10201 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
    10261 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
    10321 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
    10381 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
    10441 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
    10501 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
    10561 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
    10621 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
    10681 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
    10741 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
    10801 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
    10861 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
    10921 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
    10981 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
    11041 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
    11101 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
    11161 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
    11221 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
    11281 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
    11341 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    11401 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    11461 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    11521 cccataccct attaccactg ccaattacct agtggtttca tttactctaa acctgtgatt
    11581 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
    11641 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
    11701 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
    11761 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
    11821 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
    11881 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
    11941 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
    12001 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
    12061 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
    12121 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
    12181 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
    12241 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
    12301 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
    12361 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
    12421 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
    12481 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
    12541 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
    12601 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
    12661 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
    12721 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
    12781 cagcaagcgg ccggtgatct tcagacctgg acgatatata tgagggacaa ttggagaagt
    12841 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
    12901 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
    12961 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
    13021 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
    13081 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
    13141 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
    13201 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
    13261 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
    13321 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
    13381 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
    13441 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
    13501 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
    13561 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
    13621 agagacagat ccattcgatt agtgaacgga tctcgacggt cg
//
