LOCUS       V033419                13278 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033419
VERSION     V033419
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2734
                     /label="SHKBP1(NM_138392)"
                     /note="SHKBP1(NM_138392)"
                     /gene="SHKBP1"
     CDS             2735..2761
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2807..3148
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3274..3773
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3794..4501
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4502..4558
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4559..5158
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5169..5757
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5820..6053
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6078..6096)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6885..6989
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6990..7850
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8021..8609
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8855..9184
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10318..10383
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10513..10533
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10958..11092
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11261..11894
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11941..12066
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12558..12791
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12976..13020
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctaaccta atagcagtga
      601 actccaagcc accatggcag cagcggctac tgcagccgag ggggtcccca gtcgggggcc
      661 tcccggggaa gtcattcatc tgaatgtggg aggcaagaga ttcagtacct ctcgccagac
      721 tctcacctgg atcccagact ccttcttctc cagtcttctg agcggacgca tctcgacgct
      781 gaaagatgag accggagcaa tcttcatcga cagggaccct acagtcttcg cccccatcct
      841 caacttcctg cgcaccaaag agttggatcc caggggtgtc cacggttcca gcctcctcca
      901 tgaagcccag ttctatgggc tcactcctct ggttcgtcgc ctgcagcttc gagaggagtt
      961 ggatcgatct tcttgtggaa acgtcctctt caatggttac ctgccgccac cagtgttccc
     1021 agtgaagcgg cggaaccggc acagcctagt ggggcctcag cagctaggag gacggccagc
     1081 ccctgtccga cggagcaaca cgatgccccc caaccttggc aatgcagggc tgctgggccg
     1141 aatgctggat gagaaaaccc ctccctcacc ctcaggacaa cctgaggagc cggggatggt
     1201 gcgcctggtg tgtggacacc ataattggat cgctgtggcc tatacccagt ttctagtctg
     1261 ctacaggttg aaggaagcct ctggctggca gctggtgttt tccagccccc gcctggactg
     1321 gcccatcgaa cgactggcgc tcacagcccg ggtgcatggt ggggctttgg gtgaacatga
     1381 caagatggtg gcagcagcca ccggcagcga gatcctgcta tgggctctgc aggcggaagg
     1441 cggtggctcc gagatagggg tctttcatct gggggtgcct gtggaggcct tgttcttcgt
     1501 cgggaaccag ctcattgcta caagccacac agggcgcatc ggggtgtgga atgccgtcac
     1561 caagcactgg caggtccagg aggtgcagcc catcaccagt tatgacgcgg caggctcctt
     1621 cctcctcctg ggctgcaaca acggctccat ttactacgtg gatgtgcaga agttcccctt
     1681 gcgcatgaaa gacaacgacc tccttgtcag cgagctctat cgggacccag cggaggatgg
     1741 ggtcaccgcc ctcagtgtct acctcacccc caagaccagt gacagtggga actggatcga
     1801 gatcgcctat ggcaccagct cagggggcgt gcgggtcatc gtgcagcacc cggagactgt
     1861 gggctcgggg cctcagctct tccagacctt cactgtgcac cgcagccctg tcaccaagat
     1921 catgctgtcg gagaagcacc tcatctcagt ctgtgccgac aacaaccacg tgcggacatg
     1981 gtctgtgact cgcttccgcg gcatgatttc cacccagccc ggctccaccc cactcgcttc
     2041 ctttaagatc ctggctctgg agtcggcaga tgggcatggc ggctgcagtg ctggcaatga
     2101 cattggcccc tacggtgagc gggacgacca gcaagtgttc atccagaagg tggtgcccag
     2161 tgccagccag ctcttcgtgc gtctctcatc tactgggcag cgggtgtgct ccgtgcgctc
     2221 cgtggacggc tcacccacga cagccttcac agtgctggag tgcgagggct cccggcggct
     2281 cggctctcgg ccccggcgct acctgctcac tggccaggcc aacggcagct tggccatgtg
     2341 ggacctaacc accgccatgg acggcctcgg ccaggcccct gcaggtggcc tgacggagca
     2401 agagctgatg gaacagctgg aacactgtga gctggccccg ccggctcctt cagctccctc
     2461 atggggctgt ctccccagcc cctcaccccg catctccctc accagcctcc actcagcctc
     2521 cagcaacacc tccttgtctg gccaccgtgg gagcccaagc cccccgcagg ctgaggcccg
     2581 gcgccgtggt gggggcagct ttgtggaacg ctgccaggaa ctggtgcgga gtgggccaga
     2641 cctccgacgg ccacccacac cagccccgtg gccctccagc ggtctcggca ctcccctcac
     2701 acctcccaag atgaagctca atgaaacttc cttttacccc tacgacgtgc ctgactacgc
     2761 cggcggcggt ggttcaggcg gtggcggttc cggtggtggg ggatcagtcc aactggtgga
     2821 gtctggtggc gctttggtgc agccaggtgg ctctctgcgt ttgtcctgtg ccgcttctgg
     2881 cttcccagtg aaccgctatt ccatgcgctg gtatcgccag gctccaggca aagagcgtga
     2941 gtgggtagcc ggtatgtcca gcgcgggtga tcgtagctcc tatgaagact ccgtgaaggg
     3001 ccgtttcacc atcagccgtg acgatgcccg taacacggtg tatctgcaaa tgaacagctt
     3061 gaaacctgaa gatacggccg tgtattactg taatgtgaac gtgggcttcg agtattgggg
     3121 ccaaggcacc caggtcaccg tctccagcta agatccttga cttgcggccg caactcccac
     3181 ctgcaacatg cgtgactgac tgaggccgcg actctagagt cgacctgcat ctaggcgccg
     3241 gaattagatc tctcgaggtt aacgaattct accgggtagg ggaggcgctt ttcccaaggc
     3301 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     3361 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     3421 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     3481 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     3541 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     3601 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     3661 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     3721 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
     3781 gcccaagctt accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     3841 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     3901 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     3961 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     4021 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     4081 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     4141 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     4201 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     4261 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     4321 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     4381 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     4441 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     4501 ggcaacaaac ttctctctgc tgaaacaagc cggagatgtc gaagagaatc ctggaccgat
     4561 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     4621 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     4681 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     4741 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     4801 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     4861 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     4921 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     4981 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     5041 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     5101 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatc
     5161 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     5221 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     5281 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     5341 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     5401 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     5461 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     5521 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     5581 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     5641 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     5701 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     5761 gataccgtcg accaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     5821 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     5881 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     5941 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6001 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta
     6061 gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt
     6121 atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct
     6181 ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt
     6241 ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca
     6301 atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc
     6361 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     6421 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     6481 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
     6541 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
     6601 cgaatggtgc actctcagta caatctagct ctgatgccgc atagttaagc cagccccgac
     6661 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     6721 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     6781 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     6841 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6901 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6961 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7021 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7081 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7141 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7201 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7261 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7321 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7381 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7441 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7501 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7561 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7621 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7681 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7741 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7801 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7861 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7921 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7981 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8041 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8101 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8161 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8221 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8281 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8341 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8401 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8461 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8521 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8581 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8641 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8701 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8761 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8821 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     8881 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     8941 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     9001 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     9061 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9121 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     9181 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
     9241 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
     9301 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
     9361 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
     9421 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
     9481 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg
     9541 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
     9601 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
     9661 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
     9721 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
     9781 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
     9841 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
     9901 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
     9961 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
    10021 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca
    10081 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc
    10141 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga
    10201 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct
    10261 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
    10321 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
    10381 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
    10441 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
    10501 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
    10561 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
    10621 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
    10681 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
    10741 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
    10801 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
    10861 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
    10921 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
    10981 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    11041 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
    11101 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
    11161 ttacctagtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    11221 gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc
    11281 caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
    11341 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
    11401 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
    11461 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
    11521 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
    11581 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
    11641 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
    11701 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
    11761 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
    11821 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
    11881 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
    11941 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
    12001 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
    12061 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
    12121 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
    12181 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
    12241 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
    12301 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
    12361 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg tgatcttcag
    12421 acctggacga tatatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
    12481 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
    12541 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
    12601 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
    12661 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
    12721 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
    12781 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
    12841 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
    12901 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
    12961 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
    13021 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
    13081 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
    13141 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
    13201 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
    13261 aacggatctc gacggtcg
//
