LOCUS       V030070                14160 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030070
VERSION     V030070
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..3616
                     /label="RNF40(NM_014771)"
                     /note="RNF40(NM_014771)"
                     /gene="RNF40"
     CDS             3617..3643
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3689..4030
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        4156..4655
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4676..5383
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5384..5440
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             5441..6040
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6051..6639
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6702..6935
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6960..6978)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7767..7871
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7872..8732
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8903..9491
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9737..10066
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          11200..11265
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             11395..11415
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11840..11974
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             12143..12776
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12823..12948
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    13440..13673
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13858..13902
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttcttaggac cgtgcagtga
      601 actccaagcc accatgtctg ggccaggcaa caaacgcgcc gccggcgacg ggggctcagg
      661 gcccccggaa aagaagctga gtcgtgagga gaagaccacc acgactctta tcgagcccat
      721 tcgtcttgga ggcatctctt ccacggagga gatggacctg aaggtactac agttcaagaa
      781 caagaaactg gcagagcggc tggaacaacg gcaggcttgt gaagatgaac tccgagaacg
      841 aattgagaag ttggagaagc ggcaggccac agatgatgcc acactcctca tcgtcaatcg
      901 ctactgggcc cagctggatg aaactgtgga agcccttctc cgatgccatg agagccaggg
      961 ggagctgtct tcagcgcctg aggcacctgg gacccaggag gggccaacat gtgatgggac
     1021 tcctctccca gagccgggga catcagagct gagagacccc ttgctgatgc agctgcggcc
     1081 ccctctcagt gagccggcct tggcttttgt ggtggcactg ggtgccagca gcagtgagga
     1141 ggtggagctg gagctgcaag gccgaatgga gttctccaag gcagctgtgt ctcgtgtggt
     1201 agaggcctca gaccgcctac agcgccgggt ggaggaactc tgtcagcgag tgtacagccg
     1261 aggggacagt gagcccctca gtgaggcggc tcaggcacac acccgagagc tgggccgtga
     1321 gaaccggcga ctgcaggact tggccactca gctgcaggag aaacaccacc gcatctcatt
     1381 ggagtactcc gagctccagg ataaagtgac atcggcagag accaaggtgc tggagatgga
     1441 gacaacagtg gaggacttgc agtgggacat cgagaagctg cggaagcgag agcaaaagct
     1501 caataagcac ctggcagagg ccttagagca gcttaactct ggctactatg tatctgggag
     1561 ctcctcaggc ttccaggggg gccagatcac actcagcatg cagaagtttg agatgctgaa
     1621 tgcagagtta gaggaaaacc aggaactggc caacagccgt atggcagagc tggagaaact
     1681 gcaggccgaa cttcaggggg ctgtgcggac caatgagcgc ctcaaggtgg ccctgcggag
     1741 ccttcctgag gaggtagtgc gggagacggg ggagtaccgc atgctgcagg cccaattctc
     1801 actgctctac aacgagtctc tgcaagtgaa gacccagcta gacgaggctc ggggcctgct
     1861 gctggccaca aagaactccc acctgcgaca catcgagcac atggagagcg acgagctggg
     1921 gctgcagaag aagctacgca cagaggtcat tcagctggag gacacgctgg cccaggtacg
     1981 caaggagtat gagatgctgc gcatcgagtt tgagcagaat ctggcggcca acgagcaggc
     2041 ggggcccatc aaccgtgaga tgcgccacct gattagtagt cttcaaaacc acaaccacca
     2101 gctaaaaggg gacgcccagc gatacaagcg gaagcttcga gaagtacaag ctgagattgg
     2161 caagctccgg gcccaggcca gtggctctgc ccactccacc cccaacctgg gccacccaga
     2221 ggattctggc gtcagtgccc cagccccagg gaaagaggag ggtgggccag gccctgtcag
     2281 tacccccgac aacagaaagg agatggctcc agtgcctggc accaccacta ctaccacttc
     2341 agtgaagaag gaggagctgg tcccctctga agaggacttc cagggtataa cccctggggc
     2401 ccagggccct tcctcccggg gccgagaacc tgaggccagg cccaagcggg agcttcggga
     2461 acgggaaggt cccagcctag gacctccacc tgtagcctcc gctctctcaa gggctgatcg
     2521 ggagaaggcc aaggtggaag aaaccaagcg gaaggaatca gaactcctca agggtctccg
     2581 agcagagctc aagaaggccc aggagagcca gaaggagatg aaactgctgc tggatatgta
     2641 caagtcagcg cccaaggagc agcgggataa ggtgcagctc atggcagcgg aacgcaaggc
     2701 taaggccgag gttgatgagc tgcggagccg catccgggaa ttggaggaga gggatcgaag
     2761 ggagagcaag aagatcgcgg atgaggatgc cctgcggcgc attcggcagg cagaggagca
     2821 gatagaacac ctgcagcgca agctgggtgc caccaagcag gaggaggagg ctctgctctc
     2881 agagatggat gtgacaggtc aggcttttga ggacatgcag gaacagaacg ggcggctgct
     2941 acagcagttg cgggaaaagg atgatgccaa ctttaagcta atgtcagagc ggatcaaggc
     3001 caaccagatt cacaagctgc tgcgggagga gaaggatgag ttgggcgagc aggtccttgg
     3061 cctcaagtcc caggtggatg cccagctgct gactgtgcag aagctagagg agaaggagcg
     3121 agccttgcag ggcagcctcg ggggtgtgga gaaggagctg acgctgcgca gccaagccct
     3181 ggagctcaac aagcggaagg ctgtagaagc cgcccagctg gccgaggacc tgaaggtgca
     3241 gctggagcac gtgcagactc ggctgcggga gatccagccc tgcctggcag agagccgggc
     3301 tgctcgtgag aaagagagct tcaacctcaa gagggctcag gaggacatct cacggctgcg
     3361 gcgcaagctg gaaaagcaga ggaaggtgga ggtctacgca gatgccgacg aaatcctcca
     3421 ggaggagatc aaggagtaca aggcgcggtt gacctgcccc tgctgtaaca cccgcaagaa
     3481 ggatgcagtc cttaccaagt gcttccacgt tttctgcttc gagtgcgtgc ggggccgcta
     3541 tgaggcccgc cagaggaagt gccccaagtg caacgcggcc tttggtgccc acgacttcca
     3601 tcgtatctac atcagctacc cctacgacgt gcctgactac gccggcggcg gtggttcagg
     3661 cggtggcggt tccggtggtg ggggatcagt ccaactggtg gagtctggtg gcgctttggt
     3721 gcagccaggt ggctctctgc gtttgtcctg tgccgcttct ggcttcccag tgaaccgcta
     3781 ttccatgcgc tggtatcgcc aggctccagg caaagagcgt gagtgggtag ccggtatgtc
     3841 cagcgcgggt gatcgtagct cctatgaaga ctccgtgaag ggccgtttca ccatcagccg
     3901 tgacgatgcc cgtaacacgg tgtatctgca aatgaacagc ttgaaacctg aagatacggc
     3961 cgtgtattac tgtaatgtga acgtgggctt cgagtattgg ggccaaggca cccaggtcac
     4021 cgtctccagc taagatcctt gacttgcggc cgcaactccc acctgcaaca tgcgtgactg
     4081 actgaggccg cgactctaga gtcgacctgc atctaggcgc cggaattaga tctctcgagg
     4141 ttaacgaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     4201 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     4261 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     4321 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     4381 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     4441 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     4501 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     4561 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     4621 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatggt
     4681 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     4741 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     4801 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     4861 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     4921 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     4981 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     5041 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     5101 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     5161 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     5221 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     5281 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     5341 cgagggccgc cactccaccg gcggcatgga cgagctgtac aaggcaacaa acttctctct
     5401 gctgaaacaa gccggagatg tcgaagagaa tcctggaccg atgaccgagt acaagcccac
     5461 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     5521 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     5581 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     5641 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     5701 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     5761 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     5821 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     5881 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     5941 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     6001 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga tcttatcgat aatcaacctc
     6061 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6121 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6181 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6241 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6301 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6361 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6421 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     6481 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6541 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6601 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgaccaaggc
     6661 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     6721 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     6781 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6841 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6901 cagacccttt tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt
     6961 ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg
     7021 cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc
     7081 ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt
     7141 tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct
     7201 agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     7261 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     7321 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     7381 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     7441 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag
     7501 tacaatctag ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     7561 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     7621 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     7681 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     7741 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7801 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7861 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7921 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7981 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     8041 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     8101 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     8161 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8221 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     8281 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8341 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8401 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8461 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8521 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8581 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8641 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8701 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8761 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8821 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8881 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8941 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9001 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     9061 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9121 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9181 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9241 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9301 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9361 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9421 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9481 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9541 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9601 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9661 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     9721 aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     9781 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     9841 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     9901 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     9961 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
    10021 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa
    10081 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
    10141 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
    10201 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
    10261 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
    10321 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
    10381 gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
    10441 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
    10501 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc
    10561 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
    10621 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg
    10681 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
    10741 actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc
    10801 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
    10861 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
    10921 agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
    10981 ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga
    11041 gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca
    11101 actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca
    11161 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
    11221 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
    11281 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
    11341 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
    11401 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
    11461 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
    11521 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
    11581 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
    11641 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
    11701 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
    11761 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
    11821 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
    11881 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    11941 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
    12001 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctag tggtttcatt
    12061 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    12121 tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc
    12181 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
    12241 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
    12301 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
    12361 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
    12421 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
    12481 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
    12541 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
    12601 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
    12661 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
    12721 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
    12781 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
    12841 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
    12901 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
    12961 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
    13021 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
    13081 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
    13141 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
    13201 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
    13261 aaaagtaaga ccaccgcaca gcaagcggcc ggtgatcttc agacctggac gatatatatg
    13321 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
    13381 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
    13441 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
    13501 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
    13561 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
    13621 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
    13681 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
    13741 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
    13801 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
    13861 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
    13921 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
    13981 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
    14041 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
    14101 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtcg
//
