LOCUS       V033347                12783 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033347
VERSION     V033347
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2239
                     /label="RNF217(NM_001286398)"
                     /note="RNF217(NM_001286398)"
                     /gene="RNF217"
     CDS             2240..2266
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2312..2653
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2779..3278
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3299..4006
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4007..4063
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4064..4663
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4674..5262
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5325..5558
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5583..5601)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6390..6494
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6495..7355
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7526..8114
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8360..8689
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9823..9888
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10018..10038
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10463..10597
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10766..11399
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11446..11571
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12063..12296
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12481..12525
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttcttgtgcc tcggcagtga
      601 actccaagcc accatgggcg aggagcagag cacggtgagc ggcggcggcg ggccccagga
      661 gtcgcagacc ctggccagtg gcactgcggg ccaccctgag cccccgaggc ctcaggggga
      721 cagcgcccgg gcgcccccgc tgcgcgccgc ctccgcggag ccgagcggcg gtggctgcgg
      781 aagcgactgg ggctgcgcgg acaccagcgc cccagagccc gcgaggagcc tggggccccc
      841 gggctggagt aagagccgag caccggcgca gcctgcggga ctggcactca ccgggcctct
      901 caatccccag accttgccac tgcagttgga gctggaggag gaagaggagg aagctgggga
      961 tcgaaaagag ggaggggatg aacagcagga ggcgcccccc ggcgaagagc tggagcccag
     1021 gacccgcgtg ggggccgccg acggactggt cctggacgtg ctgggtcagc ggcgcccgtc
     1081 cctcgccaag agacaagtct tctgctccgt gtactgcgtg gagagcgacc tgcccgaggc
     1141 ccccgcctcg gagcagctct cgccgcccgc gtcgccacct ggggctccgc cagtgttgaa
     1201 ccctcccagc acccgctctt ccttccccag cccccgactg tccctcccaa cggattccct
     1261 ctcccccgac ggcggcagca tcgagctgga gttctacctg gcgcccgagc cgttctccat
     1321 gcccagcctg ttgggagctc caccctactc tggcctgggc ggtgtagggg atccctatgt
     1381 gcccctcatg gtgctgatgt gccgggtgtg cctggaagac aagcccatca agcccctgcc
     1441 ttgctgcaag aaggccgtgt gcgaggagtg cctcaaagtc tacctgagcg cccaggtaca
     1501 acttggccaa gtagaaatca aatgccccat cacagagtgt tttgaattct tggaagaaac
     1561 aactgttgtc tataacttaa cgcatgaaga ctccatcaag tataagtact tcttggaact
     1621 tggccgtatt gattccagca ccaagccatg tcctcagtgc aagcacttta caaccttcaa
     1681 gaaaaaagga catattccca ccccttccag atcagaaagc aaatacaaaa tccagtgccc
     1741 tacctgccaa ttcgtctggt gttttaagtg ccactctcct tggcatgaag gtgttaactg
     1801 caaggagtac aaaaaaggag acaaattgtt gcgtcactgg gccagcgaaa ttgagcatgg
     1861 gcagaggaat gcccagaagt gtccaaagtg caagatccac atccagcgaa ctgaaggatg
     1921 tgaccatatg acctgctcac aatgtaacac taatttttgt taccgatgtg gtgagagata
     1981 ccgccagctc cgattttttg gagaccacac atcaaacctc agtatatttg gatgcaaata
     2041 tcgctacctc ccagagagac ctcatttaag gagattagtg cgagggtcag tctgtgctgg
     2101 aaaattattc attgcacctc taattatggt tttgggattg gcactagggg ccatagcggt
     2161 tgtaatcggt ttatttgtat ttcctatcta ttgcctttgt aaaaaacaga gaaaacgatc
     2221 acggacaggt atgcactggt acccctacga cgtgcctgac tacgccggcg gcggtggttc
     2281 aggcggtggc ggttccggtg gtgggggatc agtccaactg gtggagtctg gtggcgcttt
     2341 ggtgcagcca ggtggctctc tgcgtttgtc ctgtgccgct tctggcttcc cagtgaaccg
     2401 ctattccatg cgctggtatc gccaggctcc aggcaaagag cgtgagtggg tagccggtat
     2461 gtccagcgcg ggtgatcgta gctcctatga agactccgtg aagggccgtt tcaccatcag
     2521 ccgtgacgat gcccgtaaca cggtgtatct gcaaatgaac agcttgaaac ctgaagatac
     2581 ggccgtgtat tactgtaatg tgaacgtggg cttcgagtat tggggccaag gcacccaggt
     2641 caccgtctcc agctaagatc cttgacttgc ggccgcaact cccacctgca acatgcgtga
     2701 ctgactgagg ccgcgactct agagtcgacc tgcatctagg cgccggaatt agatctctcg
     2761 aggttaacga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     2821 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     2881 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     2941 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     3001 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     3061 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     3121 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     3181 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     3241 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     3301 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     3361 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     3421 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     3481 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     3541 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     3601 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     3661 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     3721 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     3781 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     3841 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     3901 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     3961 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaaggcaa caaacttctc
     4021 tctgctgaaa caagccggag atgtcgaaga gaatcctgga ccgatgaccg agtacaagcc
     4081 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     4141 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     4201 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     4261 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     4321 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     4381 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     4441 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     4501 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     4561 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     4621 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgatcttatc gataatcaac
     4681 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     4741 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     4801 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     4861 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     4921 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     4981 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     5041 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     5101 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     5161 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     5221 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgaccaa
     5281 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5341 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     5401 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5461 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5521 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt
     5581 attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag
     5641 ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag
     5701 accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga
     5761 gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc
     5821 gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     5881 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     5941 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     6001 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     6061 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct
     6121 cagtacaatc tagctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     6181 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     6241 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     6301 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     6361 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6421 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6481 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6541 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6601 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6661 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6721 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6781 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6841 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6901 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6961 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7021 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7081 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7141 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7201 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7261 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7321 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7381 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7441 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7501 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7561 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7621 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     7681 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7741 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7801 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7861 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7921 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7981 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8041 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8101 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8161 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8221 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8281 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     8341 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     8401 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     8461 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     8521 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     8581 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     8641 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca
     8701 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
     8761 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
     8821 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
     8881 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta
     8941 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
     9001 gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt
     9061 ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga
     9121 aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt
     9181 tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg
     9241 tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc
     9301 gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct
     9361 ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta
     9421 ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct
     9481 ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg
     9541 tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga
     9601 tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga
     9661 tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg
     9721 gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg
     9781 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     9841 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     9901 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
     9961 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
    10021 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
    10081 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
    10141 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
    10201 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
    10261 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
    10321 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
    10381 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
    10441 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
    10501 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    10561 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    10621 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tagtggtttc
    10681 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    10741 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
    10801 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
    10861 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
    10921 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
    10981 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
    11041 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
    11101 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
    11161 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
    11221 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    11281 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    11341 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    11401 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
    11461 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
    11521 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
    11581 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
    11641 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
    11701 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
    11761 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
    11821 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
    11881 aacaaaagta agaccaccgc acagcaagcg gccggtgatc ttcagacctg gacgatatat
    11941 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
    12001 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
    12061 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
    12121 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
    12181 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
    12241 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
    12301 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
    12361 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
    12421 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    12481 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    12541 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    12601 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
    12661 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
    12721 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
    12781 tcg
//
