LOCUS       V033887                13926 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033887
VERSION     V033887
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..3382
                     /label="RNF216(NM_207111)"
                     /note="RNF216(NM_207111)"
                     /gene="RNF216"
     CDS             3383..3409
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3455..3796
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3922..4421
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4442..5149
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5150..5206
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             5207..5806
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5817..6405
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6468..6701
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6726..6744)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7533..7637
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7638..8498
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8669..9257
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9503..9832
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10966..11031
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             11161..11181
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11606..11740
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11909..12542
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12589..12714
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    13206..13439
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13624..13668
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctccccaa gtagcagtga
      601 actccaagcc accatggaag agggaaacaa caatgaagag gtaattcact tgaacaactt
      661 tcactgccat cggggacaag agtggatcaa tctccgagat gggcccatca ccatatctga
      721 ctcctcagat gaggaaagga ttccaatgct ggtcacccca gctcctcagc agcatgaaga
      781 agaggacctg gatgatgatg tcatcctgac agaaacaaat aaacctcaga gatcacgacc
      841 caatctcatc aaaccagctg cccagtggca agatctgaaa aggttgggag aagaaaggcc
      901 taaaaagtct agagcagcat ttgaatcaga taagagcagc tatttttcag tgtgtaacaa
      961 cccattgttt gattctgggg cacaggatga ttctgaggat gactacggtg aatttctgga
     1021 tcttgggcct cctggaatct ctgaattcac taagccaagt ggccaaacag aaagagaacc
     1081 caagcctgga ccgagtcata accaagcagc aaatgacatt gtcaacccca gatcagagca
     1141 gaaagtcatc atcttggaag aaggtagcct tctttacaca gaaagcgatc ctttggaaac
     1201 tcagaaccag tcatccgaag actcagagac agagctgtta tcaaatctag gagagtcagc
     1261 tgctctagca gatgatcagg ccatcgaaga agactgctgg ttagatcatc cttacttcca
     1321 gtctctgaac caacagcccc gtgaaataac aaaccaggtc gttcctcagg aacggcagcc
     1381 tgaagcagaa ctgggccgct tgttgtttca gcatgaattc ccagggcccg cttttccaag
     1441 gccggaaccc cagcaaggtg ggatttcagg cccctcttct cctcagcctg cccatcctct
     1501 aggagagttt gaagaccagc agttagcaag tgatgatgaa gagccaggtc cagcctttcc
     1561 aatgcaagaa tctcaagagc ccaatttgga aaacatttgg gggcaagaag ctgcagaggt
     1621 agatcaagag ctcgttgaac tactagtgaa agaaacggaa gcaagatttc cagatgtagc
     1681 aaatgggttt attgaggaaa taattcattt taagaattat tatgatctga atgtactttg
     1741 taattttctt ctggaaaacc cagattatcc aaagagagaa gacagaatca ttataaatcc
     1801 cagtagcagt ctgctggcca gccaagatga gacaaagttg cctaaaatag acttttttga
     1861 ctattctaaa ttgacccctc ttgaccagcg ctgcttcatc caagctgctg acctcctcat
     1921 ggccgacttc aaagtgctca gtagtcagga catcaagtgg gccctgcacg agctcaaagg
     1981 acactatgca atcacccgaa aggccttgtc tgatgccatt aaaaaatggc aggagctgtc
     2041 accagaaacc agtggaaaaa ggaagaagag aaaacaaatg aaccagtatt cttacattga
     2101 tttcaagttt gaacaaggtg acataaaaat agaaaagagg atgttctttc ttgaaaataa
     2161 gcgacgacat tgtaggtcct atgaccgacg tgctctcctt ccagctgtgc aacaagagca
     2221 ggagttctat gagcagaaaa tcaaagagat ggcagagcat gaagactttt tgcttgccct
     2281 acagatgaat gaagaacagt atcaaaagga tggccagctg attgagtgtc gctgctgcta
     2341 tggggaattt ccattcgagg agctgacgca gtgcgcagat gctcacttgt tctgcaaaga
     2401 gtgtctcatc agatatgccc aagaggcagt ctttggatct ggaaagttgg agctcagctg
     2461 catggaaggc agctgcacgt gttcgttccc aaccagtgag ctggagaagg tgctccccca
     2521 gaccatcctg tataagtact atgagcgaaa agccgaggag gaggttgcgg cagcctacgc
     2581 cgacgagctt gtcaggtgcc cgtcctgtag ctttccggct ctgttggaca gtgatgtgaa
     2641 gaggttcagc tgtcctaatc ctcactgccg aaaggaaacc tgtaggaagt gtcagggact
     2701 ctggaaagaa cataatggcc tcacctgtga agagctggct gaaaaagacg acatcaagta
     2761 ccgtacctct attgaagaaa aaatgactgc tgcccgcatt agaaaatgcc acaagtgtgg
     2821 gactggcctc atcaaatctg aaggctgcaa ccgcatgtct tgccgctgtg gtgcccagat
     2881 gtgctacctc tgtcgagttt ctattaatgg atatgaccat ttctgccaac atccccgctc
     2941 accaggagcc ccttgccagg agtgttcaag atgctctctc tggaccgatc ccactgaaga
     3001 tgatgagaag cttattgagg aaatccagaa ggaggctgaa gaggaacaga aaagaaagaa
     3061 tggagagaac accttcaaac gcattggacc cccgctggag aagcctgtgg agaaggtgca
     3121 gagggtggag gccctcccga ggcccgttcc gcagaacctg ccacagccac agatgccacc
     3181 ctatgccttc gcgcacccac ccttccccct gcctcccgtg cggcctgtgt tcaacaactt
     3241 cccactcaac atggggccta tcccagcccc gtacgtgccc cctctgccca acgtgcgggt
     3301 caactatgac ttcggtccca tccacatgcc cctggagcac aacctgccca tgcactttgg
     3361 cccccagccg cggcatcgct tctaccccta cgacgtgcct gactacgccg gcggcggtgg
     3421 ttcaggcggt ggcggttccg gtggtggggg atcagtccaa ctggtggagt ctggtggcgc
     3481 tttggtgcag ccaggtggct ctctgcgttt gtcctgtgcc gcttctggct tcccagtgaa
     3541 ccgctattcc atgcgctggt atcgccaggc tccaggcaaa gagcgtgagt gggtagccgg
     3601 tatgtccagc gcgggtgatc gtagctccta tgaagactcc gtgaagggcc gtttcaccat
     3661 cagccgtgac gatgcccgta acacggtgta tctgcaaatg aacagcttga aacctgaaga
     3721 tacggccgtg tattactgta atgtgaacgt gggcttcgag tattggggcc aaggcaccca
     3781 ggtcaccgtc tccagctaag atccttgact tgcggccgca actcccacct gcaacatgcg
     3841 tgactgactg aggccgcgac tctagagtcg acctgcatct aggcgccgga attagatctc
     3901 tcgaggttaa cgaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     3961 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     4021 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     4081 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     4141 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     4201 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     4261 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     4321 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     4381 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
     4441 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     4501 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     4561 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     4621 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     4681 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     4741 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     4801 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     4861 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     4921 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     4981 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     5041 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     5101 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg caacaaactt
     5161 ctctctgctg aaacaagccg gagatgtcga agagaatcct ggaccgatga ccgagtacaa
     5221 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     5281 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     5341 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     5401 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     5461 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     5521 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     5581 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     5641 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     5701 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     5761 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgatctt atcgataatc
     5821 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5881 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5941 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6001 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6061 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6121 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6181 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     6241 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6301 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     6361 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     6421 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     6481 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     6541 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6601 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6661 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
     6721 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
     6781 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
     6841 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
     6901 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
     6961 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     7021 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     7081 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     7141 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     7201 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
     7261 tctcagtaca atctagctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     7321 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     7381 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     7441 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     7501 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7561 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7621 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7681 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7741 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7801 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7861 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7921 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7981 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8041 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8101 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8161 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8221 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8281 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8341 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8401 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8461 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8521 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8581 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8641 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8701 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8761 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     8821 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8881 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8941 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9001 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9061 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9121 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9181 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9241 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9301 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9361 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9421 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9481 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     9541 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     9601 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     9661 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9721 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     9781 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
     9841 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
     9901 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
     9961 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
    10021 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
    10081 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
    10141 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
    10201 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
    10261 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
    10321 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
    10381 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
    10441 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
    10501 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa
    10561 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
    10621 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
    10681 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
    10741 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg
    10801 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg
    10861 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt
    10921 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
    10981 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
    11041 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
    11101 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
    11161 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
    11221 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
    11281 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
    11341 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
    11401 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
    11461 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
    11521 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
    11581 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
    11641 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    11701 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    11761 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctagtggt
    11821 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    11881 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
    11941 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
    12001 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
    12061 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
    12121 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
    12181 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
    12241 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
    12301 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
    12361 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    12421 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    12481 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    12541 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
    12601 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
    12661 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
    12721 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
    12781 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
    12841 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
    12901 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
    12961 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    13021 caaaacaaaa gtaagaccac cgcacagcaa gcggccggtg atcttcagac ctggacgata
    13081 tatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
    13141 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
    13201 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
    13261 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
    13321 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
    13381 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
    13441 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
    13501 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
    13561 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
    13621 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
    13681 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
    13741 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
    13801 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
    13861 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
    13921 cggtcg
//
