LOCUS       V033281                12033 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033281
VERSION     V033281
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..1489
                     /label="RNF144A(NM_014746)"
                     /note="RNF144A(NM_014746)"
                     /gene="RNF144A"
     CDS             1490..1516
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1562..1903
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2029..2528
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             2549..3256
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3257..3313
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3314..3913
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    3924..4512
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             4575..4808
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(4833..4851)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        5640..5744
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             5745..6605
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      6776..7364
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        7610..7939
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9073..9138
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9268..9288
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    9713..9847
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10016..10649
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    10696..10821
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11313..11546
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             11731..11775
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctttgcat aatgcagtga
      601 actccaagcc accatgacca caacaaggta ccggcccacc tgggacctgg ccctcgaccc
      661 gctggtgtct tgcaagctct gtcttgggga gtacccagtg gagcagatga caaccatagc
      721 ccagtgccaa tgcatcttct gtactctgtg cctgaaacag tatgttgagc tcttgatcaa
      781 agaaggatta gaaaccgcaa ttagctgccc agatgctgcc tgccctaaac agggccacct
      841 acaggagaac gagattgagt gcatggttgc agctgaaatt atgcaaagat ataaaaagct
      901 acaatttgaa agagaggtgc tgtttgatcc ctgtcggact tggtgcccgg cgtccacctg
      961 ccaagctgtg tgtcagctcc aggacgtggg gctgcagacc ccccagccag tgcagtgcaa
     1021 agcctgccgt atggaattct gctccacctg caaagccagc tggcaccctg gccagggctg
     1081 cccggagacc atgccgatca ccttcctccc cggggagacc agtgctgctt tcaaaatgga
     1141 agaagatgac gcgcccatca agcgctgccc caagtgcaaa gtctacatcg agcgagacga
     1201 aggctgcgcg cagatgatgt gcaagaactg caagcacgcc ttctgctggt actgcctgga
     1261 gtctctggac gatgatttcc ttctgataca ctacgataag ggaccctgcc ggaacaagct
     1321 gggccactcc cgggcatctg tgatctggca tcggacacag gttgtgggca tttttgcagg
     1381 atttgggctg ctgctcttgg tggcctcacc tttcctactc ctggccactc cctttgtact
     1441 ttgctgcaag tgcaagtgca gtaaaggtga cgacgacccg ttacccacct acccctacga
     1501 cgtgcctgac tacgccggcg gcggtggttc aggcggtggc ggttccggtg gtgggggatc
     1561 agtccaactg gtggagtctg gtggcgcttt ggtgcagcca ggtggctctc tgcgtttgtc
     1621 ctgtgccgct tctggcttcc cagtgaaccg ctattccatg cgctggtatc gccaggctcc
     1681 aggcaaagag cgtgagtggg tagccggtat gtccagcgcg ggtgatcgta gctcctatga
     1741 agactccgtg aagggccgtt tcaccatcag ccgtgacgat gcccgtaaca cggtgtatct
     1801 gcaaatgaac agcttgaaac ctgaagatac ggccgtgtat tactgtaatg tgaacgtggg
     1861 cttcgagtat tggggccaag gcacccaggt caccgtctcc agctaagatc cttgacttgc
     1921 ggccgcaact cccacctgca acatgcgtga ctgactgagg ccgcgactct agagtcgacc
     1981 tgcatctagg cgccggaatt agatctctcg aggttaacga attctaccgg gtaggggagg
     2041 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     2101 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     2161 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     2221 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     2281 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     2341 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     2401 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     2461 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     2521 gcctttcgac ctgcagccca agcttaccat ggtgagcaag ggcgaggagg ataacatggc
     2581 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     2641 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     2701 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     2761 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     2821 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     2881 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     2941 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     3001 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     3061 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     3121 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     3181 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     3241 ggacgagctg tacaaggcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga
     3301 gaatcctgga ccgatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     3361 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     3421 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     3481 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     3541 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     3601 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     3661 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     3721 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     3781 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     3841 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     3901 gcccggtgcc tgatcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     3961 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     4021 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     4081 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     4141 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     4201 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     4261 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     4321 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     4381 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     4441 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     4501 ccgcctcccc gcatcgatac cgtcgaccaa ggcagctgta gatcttagcc actttttaaa
     4561 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     4621 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     4681 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     4741 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     4801 ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt
     4861 atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct
     4921 aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc
     4981 agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa
     5041 atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc
     5101 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     5161 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     5221 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     5281 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     5341 cataagggag agcgtcgaat ggtgcactct cagtacaatc tagctctgat gccgcatagt
     5401 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     5461 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     5521 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     5581 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     5641 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5701 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5761 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5821 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5881 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5941 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6001 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6061 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6121 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6181 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6241 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6301 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6361 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6421 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6481 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6541 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6601 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6661 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6721 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6781 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6841 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6901 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     6961 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7021 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7081 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7141 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7201 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7261 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7321 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7381 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7441 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     7501 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     7561 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt
     7621 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7681 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7741 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     7801 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7861 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7921 aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa
     7981 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
     8041 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
     8101 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
     8161 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
     8221 aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg
     8281 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     8341 gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     8401 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     8461 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     8521 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     8581 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     8641 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     8701 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     8761 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     8821 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg
     8881 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     8941 tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc
     9001 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
     9061 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
     9121 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
     9181 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
     9241 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
     9301 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
     9361 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
     9421 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt
     9481 tttcttactc cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt
     9541 acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg
     9601 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     9661 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     9721 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     9781 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     9841 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     9901 tattaccact gccaattacc tagtggtttc atttactcta aacctgtgat tcctctgaat
     9961 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa
    10021 gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa
    10081 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
    10141 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
    10201 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
    10261 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
    10321 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
    10381 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
    10441 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
    10501 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
    10561 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    10621 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
    10681 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
    10741 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
    10801 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
    10861 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
    10921 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
    10981 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
    11041 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
    11101 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
    11161 gccggtgatc ttcagacctg gacgatatat atgagggaca attggagaag tgaattatat
    11221 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
    11281 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
    11341 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
    11401 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
    11461 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
    11521 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
    11581 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
    11641 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
    11701 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
    11761 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
    11821 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
    11881 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
    11941 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
    12001 tccattcgat tagtgaacgg atctcgacgg tcg
//
