LOCUS       V030060                13149 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030060
VERSION     V030060
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2605
                     /label="RNF139(NM_007218)"
                     /note="RNF139(NM_007218)"
                     /gene="RNF139"
     CDS             2606..2632
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2678..3019
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3145..3644
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3665..4372
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4373..4429
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4430..5029
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5040..5628
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5691..5924
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5949..5967)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6756..6860
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6861..7721
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7892..8480
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8726..9055
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10189..10254
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10384..10404
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10829..10963
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11132..11765
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11812..11937
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12429..12662
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12847..12891
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctatacga attgcagtga
      601 actccaagcc accatggcgg ccgtggggcc cccgcagcag caggtgcgga tggcccatca
      661 gcaggtctgg gcggcgctcg aagtggcgct ccgggtgccc tgcctttaca tcatcgacgc
      721 catcttcaac tcctacccgg attccagcca aagccggttc tgcatcgtgc tccagatctt
      781 cctccggctc tttggtgtat ttgcatccag tattgttctg atcttgtcac aacgatcact
      841 tttcaagttt tacacgtaca gctcagcctt tctgttagct gcaacttcag tgttggtgaa
      901 ttattatgct tctttgcaca ttgacttcta tggtgcctac aacacgtcag cttttggaat
      961 tgagctgctt cctcgaaaag gtccctcgct gtggatggca cttatcgttc tacagctaac
     1021 atttggaatt ggatacgtta cactactcca gattcattcc atctattcac aattaattat
     1081 tttggatctc ttggttcctg taataggctt aatcacagag ctaccattac acatcagaga
     1141 gactttactg tttacttctt ccttgattct cacattaaat acagtgtttg tcctggcagt
     1201 gaaactgaag tggttttatt attccacacg atatgtttat cttttggtga ggcacatgta
     1261 tcgaatttac ggattacagt tattgatgga ggacacatgg aagaggattc gtttcccaga
     1321 catactacga gtcttttggc taacaagagt tacagctcag gctacagtgt taatgtacat
     1381 cttaaggatg gcaaatgaaa ctgattcctt ctttatttct tgggatgatt tttgggacct
     1441 catttgcaat cttataatta gtgggtgcga ttctacacta actgtactgg gcatgagtgc
     1501 tgtaatttcc tcagtagccc attatttggg gcttggaata ttggccttta ttggatcaac
     1561 tgaggaagat gacaggcgtc ttggctttgt tgcacctgtt ttatttttta ttttggctct
     1621 tcagactggg ttaagtgggc taagaccaga agagagactt attcgcttaa gtagaaacat
     1681 gtgcctttta ttaactgcag tcctgcattt tatccatgga atgacagacc ctgtattaat
     1741 gtctctcagt gcctctcatg tgtcatcttt tcgtagacat tttcctgtgc tgtttgtctc
     1801 tgcttgcctg tttattcttc ctgtcttact cagttatgtt ctttggcatc actatgcact
     1861 aaatacatgg ttgtttgcag ttacagcatt ttgtgtggaa ctgtgcttaa aagtaattgt
     1921 ttctctcact gtttatacgt tattcatgat tgatggctac tataatgtcc tctgggaaaa
     1981 gcttgacgat tatgtctact acgttcgttc aacaggcagt attattgaat ttatatttgg
     2041 agttgtaatg tttggaaatg gggcttacac tatgatgttt gagtcgggaa gtaaaattcg
     2101 ggcttttatg atgtgcctac atgcatattt taacatctac ttacaagcca aaaatggctg
     2161 gaagacattt atgaatcgta ggactgctgt gaagaaaatt aattcacttc ctgaaataaa
     2221 agggagccgc ttacaagaaa taaatgatgt atgtgcaatc tgctatcatg agtttacaac
     2281 atctgctcgt attacaccgt gtaatcatta tttccatgca ctttgccttc ggaaatggct
     2341 gtacattcaa gatacttgtc caatgtgcca tcagaaagta tacatcgaag atgatatcaa
     2401 ggataattca aatgtatcta acaacaatgg atttattcca cccaatgaaa ctccagagga
     2461 agctgtaaga gaagctgctg ctgaatctga cagggaattg aacgaagatg acagtacaga
     2521 ttgtgatgat gatgttcaaa gagaaagaaa tggagtgatt cagcacacag gcgcagcagc
     2581 tgaagaattt aatgatgata ctgactaccc ctacgacgtg cctgactacg ccggcggcgg
     2641 tggttcaggc ggtggcggtt ccggtggtgg gggatcagtc caactggtgg agtctggtgg
     2701 cgctttggtg cagccaggtg gctctctgcg tttgtcctgt gccgcttctg gcttcccagt
     2761 gaaccgctat tccatgcgct ggtatcgcca ggctccaggc aaagagcgtg agtgggtagc
     2821 cggtatgtcc agcgcgggtg atcgtagctc ctatgaagac tccgtgaagg gccgtttcac
     2881 catcagccgt gacgatgccc gtaacacggt gtatctgcaa atgaacagct tgaaacctga
     2941 agatacggcc gtgtattact gtaatgtgaa cgtgggcttc gagtattggg gccaaggcac
     3001 ccaggtcacc gtctccagct aagatccttg acttgcggcc gcaactccca cctgcaacat
     3061 gcgtgactga ctgaggccgc gactctagag tcgacctgca tctaggcgcc ggaattagat
     3121 ctctcgaggt taacgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     3181 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     3241 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     3301 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     3361 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     3421 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     3481 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     3541 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     3601 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct
     3661 taccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     3721 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     3781 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     3841 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     3901 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     3961 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     4021 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     4081 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     4141 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     4201 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     4261 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     4321 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca aggcaacaaa
     4381 cttctctctg ctgaaacaag ccggagatgt cgaagagaat cctggaccga tgaccgagta
     4441 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     4501 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     4561 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     4621 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     4681 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     4741 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     4801 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     4861 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     4921 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     4981 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgat cttatcgata
     5041 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5101 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5161 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5221 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5281 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5341 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5401 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     5461 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5521 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5581 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     5641 gaccaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5701 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     5761 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5821 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5881 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt
     5941 ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa
     6001 ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg
     6061 aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc
     6121 ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg
     6181 gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
     6241 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
     6301 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
     6361 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
     6421 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg
     6481 cactctcagt acaatctagc tctgatgccg catagttaag ccagccccga cacccgccaa
     6541 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     6601 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     6661 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     6721 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6781 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6841 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6901 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6961 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7021 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7081 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     7141 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7201 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7261 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7321 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7381 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7441 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7501 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7561 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7621 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7681 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7741 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7801 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7861 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7921 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7981 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     8041 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8101 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8161 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8221 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8281 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8341 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8401 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8461 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8521 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8581 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8641 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     8701 cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     8761 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     8821 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8881 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8941 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     9001 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     9061 tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga
     9121 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc
     9181 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
     9241 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
     9301 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
     9361 gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     9421 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     9481 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     9541 tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     9601 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga
     9661 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     9721 acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag
     9781 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     9841 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     9901 cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     9961 gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta
    10021 ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc
    10081 gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt
    10141 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
    10201 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
    10261 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
    10321 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
    10381 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
    10441 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
    10501 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
    10561 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
    10621 gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
    10681 ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac
    10741 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
    10801 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
    10861 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    10921 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    10981 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctagt
    11041 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    11101 tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga
    11161 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
    11221 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
    11281 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
    11341 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
    11401 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
    11461 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
    11521 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
    11581 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
    11641 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
    11701 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
    11761 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
    11821 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
    11881 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
    11941 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
    12001 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
    12061 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
    12121 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
    12181 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
    12241 gagcaaaaca aaagtaagac caccgcacag caagcggccg gtgatcttca gacctggacg
    12301 atatatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
    12361 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
    12421 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
    12481 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
    12541 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
    12601 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
    12661 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
    12721 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
    12781 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
    12841 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
    12901 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
    12961 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
    13021 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
    13081 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
    13141 cgacggtcg
//
