LOCUS       V033474                11946 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033474
VERSION     V033474
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..1402
                     /label="RFPL4B(NM_001013734)"
                     /note="RFPL4B(NM_001013734)"
                     /gene="RFPL4B"
     CDS             1403..1429
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1475..1816
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        1942..2441
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             2462..3169
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3170..3226
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3227..3826
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    3837..4425
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             4488..4721
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(4746..4764)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        5553..5657
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             5658..6518
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      6689..7277
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        7523..7852
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          8986..9051
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9181..9201
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    9626..9760
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             9929..10562
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    10609..10734
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11226..11459
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             11644..11688
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctacatct gaggcagtga
      601 actccaagcc accatggcca aacgcctgca agcagagttg tcctgtccag tttgcctgga
      661 ttttttctcc tgttccattt ctctctcttg tacacacgtg ttctgctttg attgcatcca
      721 gaggtatata ctagaaaacc atgattttag agcgatgtgc cccttgtgtc gagacgtggt
      781 gaaggtacct gctttggaag aatggcaagt gagcgtccta acacttatga ccaagcagca
      841 caatagccga cttgagcaaa gtctgcacgt gagggaggag ctccggcatt ttcgggagga
      901 tgtgaccctg gatgcagcca ctgccagctc cctccttgtc ttctccaatg atctaagaag
      961 cgctcagtgt aagaagatcc accacgatct gacaaaagat cccaggctgg cctgtgtcct
     1021 gggtactccc tgcttctcct ccggccaaca ttactgggag gttgaagtgg gagaggtgaa
     1081 gtcatggtcc ctgggcgtct gcaaggagcc ggctgacaga aagagcaatg atttattccc
     1141 tgagcatggc ttctggatca gcatgaaggc aggagcaatc catgctaaca cccacctgga
     1201 gagaattcct gcaagccctc gccttcgccg tgtgggaatt ttcctggatg ctgacttaga
     1261 agaaatccag ttttttgatg ttgacaataa tgtcctcatc tatacacatg atggtttctt
     1321 ctctttggag cttttgtgtc cattcttctg tcttgagctc ttgggagaag gggagagtgg
     1381 caacgtcctg accatctgcc cataccccta cgacgtgcct gactacgccg gcggcggtgg
     1441 ttcaggcggt ggcggttccg gtggtggggg atcagtccaa ctggtggagt ctggtggcgc
     1501 tttggtgcag ccaggtggct ctctgcgttt gtcctgtgcc gcttctggct tcccagtgaa
     1561 ccgctattcc atgcgctggt atcgccaggc tccaggcaaa gagcgtgagt gggtagccgg
     1621 tatgtccagc gcgggtgatc gtagctccta tgaagactcc gtgaagggcc gtttcaccat
     1681 cagccgtgac gatgcccgta acacggtgta tctgcaaatg aacagcttga aacctgaaga
     1741 tacggccgtg tattactgta atgtgaacgt gggcttcgag tattggggcc aaggcaccca
     1801 ggtcaccgtc tccagctaag atccttgact tgcggccgca actcccacct gcaacatgcg
     1861 tgactgactg aggccgcgac tctagagtcg acctgcatct aggcgccgga attagatctc
     1921 tcgaggttaa cgaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     1981 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     2041 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     2101 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     2161 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     2221 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     2281 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     2341 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     2401 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
     2461 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     2521 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     2581 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     2641 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     2701 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     2761 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     2821 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     2881 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     2941 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     3001 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     3061 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     3121 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg caacaaactt
     3181 ctctctgctg aaacaagccg gagatgtcga agagaatcct ggaccgatga ccgagtacaa
     3241 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     3301 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     3361 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     3421 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     3481 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     3541 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     3601 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     3661 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     3721 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     3781 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgatctt atcgataatc
     3841 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     3901 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     3961 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     4021 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     4081 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     4141 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     4201 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     4261 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     4321 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     4381 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     4441 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     4501 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     4561 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4621 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4681 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
     4741 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
     4801 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
     4861 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
     4921 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
     4981 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     5041 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     5101 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     5161 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     5221 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
     5281 tctcagtaca atctagctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     5341 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     5401 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     5461 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     5521 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5581 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5641 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5701 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5761 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5821 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5881 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5941 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6001 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6061 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6121 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6181 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6241 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6301 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6361 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6421 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6481 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6541 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6601 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6661 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6721 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6781 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     6841 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6901 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6961 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7021 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7081 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7141 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7201 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7261 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7321 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7381 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7441 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7501 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     7561 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     7621 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     7681 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7741 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7801 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
     7861 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
     7921 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
     7981 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
     8041 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
     8101 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
     8161 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     8221 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
     8281 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
     8341 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
     8401 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
     8461 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
     8521 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa
     8581 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
     8641 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
     8701 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
     8761 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg
     8821 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg
     8881 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt
     8941 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
     9001 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
     9061 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
     9121 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
     9181 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
     9241 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
     9301 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
     9361 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
     9421 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
     9481 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
     9541 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     9601 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     9661 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     9721 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
     9781 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctagtggt
     9841 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
     9901 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
     9961 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
    10021 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
    10081 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
    10141 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
    10201 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
    10261 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
    10321 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
    10381 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    10441 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    10501 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    10561 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
    10621 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
    10681 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
    10741 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
    10801 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
    10861 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
    10921 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
    10981 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    11041 caaaacaaaa gtaagaccac cgcacagcaa gcggccggtg atcttcagac ctggacgata
    11101 tatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
    11161 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
    11221 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
    11281 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
    11341 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
    11401 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
    11461 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
    11521 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
    11581 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
    11641 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
    11701 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
    11761 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
    11821 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
    11881 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
    11941 cggtcg
//
