LOCUS       V033391                12018 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033391
VERSION     V033391
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..1474
                     /label="RFPL4A(NM_001145014)"
                     /note="RFPL4A(NM_001145014)"
                     /gene="RFPL4A"
     CDS             1475..1501
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1547..1888
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2014..2513
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             2534..3241
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3242..3298
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3299..3898
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    3909..4497
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             4560..4793
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(4818..4836)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        5625..5729
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             5730..6590
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      6761..7349
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        7595..7924
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9058..9123
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9253..9273
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    9698..9832
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10001..10634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    10681..10806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11298..11531
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             11716..11760
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctcctccc atcgcagtga
      601 actccaagcc accatggctg agcacttcaa acagatcatt agatgtcctg tctgtctaaa
      661 agatcttgaa gaagccgtgc aactgaaatg tggatatgcc tgctgcctcc agtgcctcaa
      721 ttcactccag aaggagcccg atggggaagg tttactgtgc cgtttctgct ctgtggtctc
      781 tcagaaggat gacatcaagc ccaagtacaa gctgagggcg ctggtttcca tcatcaagga
      841 actagagccc aagctgaaat ctgttctaac aatgaaccca aggatgagga agtttcaagt
      901 ggatatgacg ttcgatgtgg acacagccaa caactatctc atcatttctg aagacctgag
      961 gagtttccga agtggggatt tgagccagaa taggaaggag caagctgaga ggttcgacac
     1021 tgccctgtgc gtcctgggca cccctcgctt cacttccggc cgccattact gggaggtgga
     1081 cgtgggcacc agccaagtgt gggatgtggg cgtgtgcaag gaatctgtga accgacaggg
     1141 gaagattgtg ctttcttcag aacacggctt cttgactgtg ggttgcagag aaggaaaggt
     1201 ctttgctgcc agcactgtgc ctatgactcc tctctgggtg agtccccagt tgcacagagt
     1261 ggggattttc ctggatgtag gtatgaggtc cattgccttt tacaatgtta gtgatgggtg
     1321 ccatatctac acattcatcg agattcctgt ttgcgagccc tggcgtccat tttttgctca
     1381 taaacgtgga agtcaagatg atcagagcat cctgagtatc tgttctgtga tcaatccatc
     1441 cgctgccagt gccccagttt cttctgaggg aaagtacccc tacgacgtgc ctgactacgc
     1501 cggcggcggt ggttcaggcg gtggcggttc cggtggtggg ggatcagtcc aactggtgga
     1561 gtctggtggc gctttggtgc agccaggtgg ctctctgcgt ttgtcctgtg ccgcttctgg
     1621 cttcccagtg aaccgctatt ccatgcgctg gtatcgccag gctccaggca aagagcgtga
     1681 gtgggtagcc ggtatgtcca gcgcgggtga tcgtagctcc tatgaagact ccgtgaaggg
     1741 ccgtttcacc atcagccgtg acgatgcccg taacacggtg tatctgcaaa tgaacagctt
     1801 gaaacctgaa gatacggccg tgtattactg taatgtgaac gtgggcttcg agtattgggg
     1861 ccaaggcacc caggtcaccg tctccagcta agatccttga cttgcggccg caactcccac
     1921 ctgcaacatg cgtgactgac tgaggccgcg actctagagt cgacctgcat ctaggcgccg
     1981 gaattagatc tctcgaggtt aacgaattct accgggtagg ggaggcgctt ttcccaaggc
     2041 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     2101 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     2161 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     2221 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     2281 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     2341 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     2401 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     2461 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
     2521 gcccaagctt accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     2581 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     2641 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     2701 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     2761 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     2821 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     2881 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     2941 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     3001 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     3061 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     3121 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     3181 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     3241 ggcaacaaac ttctctctgc tgaaacaagc cggagatgtc gaagagaatc ctggaccgat
     3301 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     3361 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     3421 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     3481 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     3541 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     3601 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     3661 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     3721 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     3781 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     3841 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatc
     3901 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     3961 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4021 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4081 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4141 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4201 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4261 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     4321 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4381 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     4441 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     4501 gataccgtcg accaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     4561 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     4621 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     4681 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     4741 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta
     4801 gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt
     4861 atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct
     4921 ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt
     4981 ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca
     5041 atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc
     5101 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     5161 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     5221 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
     5281 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
     5341 cgaatggtgc actctcagta caatctagct ctgatgccgc atagttaagc cagccccgac
     5401 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     5461 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     5521 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     5581 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5641 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5701 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5761 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5821 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5881 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5941 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6001 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6061 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6121 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6181 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6241 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6301 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6361 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6421 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6481 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6541 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6601 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6661 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6721 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6781 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6841 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6901 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6961 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7021 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7081 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7141 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7201 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7261 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7321 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7381 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7441 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7501 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7561 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     7621 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     7681 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7741 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     7801 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7861 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     7921 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
     7981 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
     8041 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
     8101 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
     8161 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
     8221 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg
     8281 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
     8341 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
     8401 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
     8461 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
     8521 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
     8581 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
     8641 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
     8701 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
     8761 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca
     8821 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc
     8881 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga
     8941 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct
     9001 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
     9061 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
     9121 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
     9181 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
     9241 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
     9301 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
     9361 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
     9421 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
     9481 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
     9541 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
     9601 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     9661 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     9721 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     9781 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
     9841 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
     9901 ttacctagtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
     9961 gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc
    10021 caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
    10081 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
    10141 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
    10201 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
    10261 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
    10321 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
    10381 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
    10441 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
    10501 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
    10561 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
    10621 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
    10681 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
    10741 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
    10801 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
    10861 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
    10921 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
    10981 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
    11041 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
    11101 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg tgatcttcag
    11161 acctggacga tatatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
    11221 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
    11281 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
    11341 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
    11401 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
    11461 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
    11521 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
    11581 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
    11641 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
    11701 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
    11761 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
    11821 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
    11881 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
    11941 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
    12001 aacggatctc gacggtcg
//
