LOCUS       V033662                12750 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033662
VERSION     V033662
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2206
                     /label="RCBTB1(NM_018191)"
                     /note="RCBTB1(NM_018191)"
                     /gene="RCBTB1"
     CDS             2207..2233
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2279..2620
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2746..3245
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3266..3973
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3974..4030
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4031..4630
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4641..5229
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5292..5525
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5550..5568)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6357..6461
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6462..7322
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7493..8081
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8327..8656
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9790..9855
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9985..10005
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10430..10564
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10733..11366
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11413..11538
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12030..12263
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12448..12492
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctaaactg cgtgcagtga
      601 actccaagcc accatggtgg atgtcggaaa gtggcccatc ttcactctac tctcccctca
      661 agagatcgcg tctattcgga aggcgtgtgt cttcggcacc tcagccagtg aagcactgta
      721 cgttactgac aatgatgagg tctttgtatt tggactgaac tatagtaact gtctaggaac
      781 tggagataac cagagtacac ttgtacccaa aaagctagaa ggcttatgtg gaaagaagat
      841 taaaagcctc agttacggga gtggaccaca tgttcttctc agcaccgaag atggagtggt
      901 ttatgcctgg ggccacaatg gatatagcca gcttgggaat gggacgacca accaaggcat
      961 tgctcccgtc caggtctgta ccaatctctt gatcaagcaa gtggtggaag tagcttgtgg
     1021 ctcacatcat tcaatggctc tggcagctga tggagaggtg tttgcttggg gttataacaa
     1081 ctgtggccaa gtgggatcag gttctacagc aaatcaacca actcctcgaa aagttacaaa
     1141 ctgtttacat attaagaggg tagttggcat tgcctgtggt cagacttcat ccatggctgt
     1201 tctggacaat ggcgaggtat atggctgggg ttacaatggc aacggtcagc tgggcctggg
     1261 aaacaatggc aaccagctga cccctgtgag agtggcagct ttgcacagcg tgtgtgtgaa
     1321 ccagattgtc tgcggttacg cacatactct agcactaaca gatgagggct tgctgtatgc
     1381 ctggggagct aacacatatg ggcagctggg aactggcaat aaaaataacc tgctaagccc
     1441 agcacacatc atggtggaga aagaaagggt ggtagagatt gcagcctgtc actctgccca
     1501 cacgtctgca gccaagacgc agggtgggca cgtgtacatg tggggccagt gccggggtca
     1561 gtccgtgatc ctcccgcacc tcacccactt ctcctgcacc gacgacgtgt ttgcctgctt
     1621 tgccactccc gccgtctcgt ggcgcctcct gtctgtggag catgaagact ttttaacagt
     1681 tgcagagtca ctgaagaaag aatttgatag tccagaaact gctgatctga agtttcgaat
     1741 tgatggaaaa tatattcatg tccataaagc tgttttgaaa atcaggtgtg agcattttcg
     1801 atccatgttc cagtcgtatt ggaatgaaga catgaaggaa gtgatagaaa tcgatcagtt
     1861 ttcttaccca gtgtatcgtg cctttctcca gtacctctac acagacacag tcgacctgcc
     1921 gccagaagat gctataggtc ttctggattt ggcgacatct tactgtgaaa acagactgaa
     1981 aaaactttgt cagcacatta tcaagagagg aattactgtg gagaatgcct tttcgctatt
     2041 ctctgctgca gtcagatatg atgcagagga tttagaagaa ttctgcttta agttttgcat
     2101 caatcatttg acagaagtta cacagactgc agcattttgg caaatggatg gccctctgct
     2161 aaaggaattc attgctaaag ccagtaaatg tggagccttt aagaactacc cctacgacgt
     2221 gcctgactac gccggcggcg gtggttcagg cggtggcggt tccggtggtg ggggatcagt
     2281 ccaactggtg gagtctggtg gcgctttggt gcagccaggt ggctctctgc gtttgtcctg
     2341 tgccgcttct ggcttcccag tgaaccgcta ttccatgcgc tggtatcgcc aggctccagg
     2401 caaagagcgt gagtgggtag ccggtatgtc cagcgcgggt gatcgtagct cctatgaaga
     2461 ctccgtgaag ggccgtttca ccatcagccg tgacgatgcc cgtaacacgg tgtatctgca
     2521 aatgaacagc ttgaaacctg aagatacggc cgtgtattac tgtaatgtga acgtgggctt
     2581 cgagtattgg ggccaaggca cccaggtcac cgtctccagc taagatcctt gacttgcggc
     2641 cgcaactccc acctgcaaca tgcgtgactg actgaggccg cgactctaga gtcgacctgc
     2701 atctaggcgc cggaattaga tctctcgagg ttaacgaatt ctaccgggta ggggaggcgc
     2761 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     2821 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     2881 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     2941 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     3001 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     3061 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     3121 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     3181 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     3241 tttcgacctg cagcccaagc ttaccatggt gagcaagggc gaggaggata acatggccat
     3301 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     3361 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     3421 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     3481 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     3541 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     3601 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     3661 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     3721 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     3781 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     3841 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     3901 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     3961 cgagctgtac aaggcaacaa acttctctct gctgaaacaa gccggagatg tcgaagagaa
     4021 tcctggaccg atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     4081 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     4141 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     4201 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     4261 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     4321 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     4381 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     4441 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     4501 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     4561 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     4621 cggtgcctga tcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     4681 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4741 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4801 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4861 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4921 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4981 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5041 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5101 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5161 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5221 cctccccgca tcgataccgt cgaccaaggc agctgtagat cttagccact ttttaaaaga
     5281 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     5341 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     5401 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     5461 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     5521 tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg
     5581 atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat
     5641 tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga
     5701 gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca cccggaaatg
     5761 gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg
     5821 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     5881 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     5941 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     6001 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     6061 aagggagagc gtcgaatggt gcactctcag tacaatctag ctctgatgcc gcatagttaa
     6121 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     6181 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     6241 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     6301 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     6361 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6421 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6481 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6541 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6601 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6661 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     6721 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6781 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6841 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6901 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6961 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     7021 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     7081 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7141 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7201 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7261 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7321 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7381 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7441 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7501 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7561 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7621 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7681 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7741 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7801 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7861 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7921 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7981 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8041 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8101 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8161 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8221 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     8281 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg
     8341 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8401 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     8461 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     8521 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8581 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     8641 cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac
     8701 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
     8761 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
     8821 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
     8881 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
     8941 ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga
     9001 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
     9061 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
     9121 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
     9181 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
     9241 cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
     9301 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
     9361 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
     9421 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
     9481 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
     9541 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa
     9601 tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat
     9661 acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt
     9721 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     9781 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     9841 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     9901 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     9961 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
    10021 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
    10081 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
    10141 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
    10201 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
    10261 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
    10321 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
    10381 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
    10441 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    10501 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    10561 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
    10621 taccactgcc aattacctag tggtttcatt tactctaaac ctgtgattcc tctgaattat
    10681 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
    10741 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
    10801 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
    10861 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
    10921 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
    10981 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
    11041 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
    11101 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
    11161 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
    11221 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    11281 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
    11341 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
    11401 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
    11461 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
    11521 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
    11581 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
    11641 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
    11701 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
    11761 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
    11821 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
    11881 ggtgatcttc agacctggac gatatatatg agggacaatt ggagaagtga attatataaa
    11941 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
    12001 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
    12061 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
    12121 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
    12181 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
    12241 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
    12301 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
    12361 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
    12421 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
    12481 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
    12541 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
    12601 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
    12661 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
    12721 attcgattag tgaacggatc tcgacggtcg
//
