LOCUS       V033437                14730 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033437
VERSION     V033437
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..4186
                     /label="RC3H2(NM_001100588)"
                     /note="RC3H2(NM_001100588)"
                     /gene="RC3H2"
     CDS             4187..4213
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4259..4600
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        4726..5225
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5246..5953
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5954..6010
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             6011..6610
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6621..7209
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7272..7505
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(7530..7548)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        8337..8441
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             8442..9302
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      9473..10061
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        10307..10636
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          11770..11835
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             11965..11985
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    12410..12544
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             12713..13346
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    13393..13518
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    14010..14243
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             14428..14472
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctattgac ctagcagtga
      601 actccaagcc accatgcctg tgcaggcagc tcaatggaca gaatttctgt cctgtccaat
      661 ctgctataat gaatttgatg agaatgtgca caaacccatc agtttaggtt gttcacacac
      721 tgtttgcaag acctgcttga ataaacttca tcgaaaagct tgtccttttg accagactgc
      781 catcaacaca gatattgatg tacttcctgt caacttcgca cttctccagt tagttggagc
      841 ccaggtacca gatcatcagt caattaagtt aagtaatcta ggtgagaata aacactatga
      901 ggttgcaaag aaatgcgttg aggatttggc actctactta aaaccactaa gtggaggtaa
      961 aggtgtagct agcttgaacc agagtgcact gagccgtcca atgcaaagga aactggtgac
     1021 acttgtaaac tgtcaactgg tggaggaaga aggtcgtgta agagccatgc gagcagctcg
     1081 ttcccttgga gaaagaactg taacagaact gatattacag caccagaacc ctcagcagtt
     1141 gtctgccaat ctatgggccg ctgtcagggc tcgaggatgc cagtttttag ggccagctat
     1201 gcaagaagag gccttgaagc tggtgttact ggcattagaa gatggttctg ccctctcaag
     1261 gaaagttctg gtactttttg ttgtgcagag actagaacca agatttcctc aggcatcaaa
     1321 aacaagtatt ggtcatgttg tgcaactact gtatcgagct tcttgtttta aggttaccaa
     1381 aagagatgaa gactcttccc taatgcagct gaaggaggaa tttcggagtt atgaagcatt
     1441 acgcagagaa catgatgccc aaattgttca tattgccatg gaagcaggac tccgtatttc
     1501 acctgaacag tggtcctctc ttttgtatgg tgatttggct cataaatcac acatgcagtc
     1561 tatcattgat aagctacagt ctccagagtc atttgcaaag agtgtccagg aattgacaat
     1621 tgttttgcaa cgaacaggtg acccagctaa cttaaataga ctgaggcctc atttagagct
     1681 tcttgcaaac atagacccta atccagacgc tgtttcacca acttgggagc agctggaaaa
     1741 tgcaatggta gctgttaaaa cagtagttca tggccttgtg gacttcatac aaaattatag
     1801 tagaaaaggc catgagaccc ctcagcctca gccaaacagc aaatacaaga ctagcatgtg
     1861 ccgagatttg cgacagcaag ggggttgtcc acgaggaaca aattgtacat ttgcccattc
     1921 tcaggaagag cttgaaaagt atcgattaag gaacaaaaag atcaatgcca ctgtaagaac
     1981 gtttcctctt ctaaataaag ttggtgtaaa caacactgtc acaaccacag ccggaaatgt
     2041 catttctgtc ataggaagta ctgaaacaac agggaaaatt gttccaagta caaacggaat
     2101 ttcaaatgca gaaaacagtg tttcccagct aatctcacgt agtactgaca gtaccttaag
     2161 agctctggag accgtgaaga aagtgggaaa ggttggcgct aatggtcaga atgctgctgg
     2221 gccctctgca gattctgtaa ctgaaaataa aattggttct ccacccaaga ctcctgtaag
     2281 taatgtagca gctacctcag ctgggccctc taatgttgga acagagctga attctgtgcc
     2341 tcaaaaatcc agcccatttc taactagagt accagtatat cctccgcatt ctgaaaacat
     2401 tcagtatttt caagatccaa ggactcagat accctttgaa gtcccacagt acccacagac
     2461 aggatactat ccaccacctc caacggtacc agctggtgtg gctccctgtg ttcctcgctt
     2521 tgtgaggtcc aataacgttc cagagtcctc cctcccacct gcttccatgc catatgccga
     2581 tcattacagt acattttccc ctcgagatcg aatgaattct tctccttacc agcctcctcc
     2641 tccgcagccg tatggaccag ttcctccagt accttctgga atgtatgctc ctgtgtacga
     2701 cagcaggcgc atctggcgcc cacctatgta ccaacgagat gacattatta gaagcaattc
     2761 tttacctcca atggatgtga tgcactcatc tgtctatcag acatctttgc gggaaagata
     2821 taactcatta gatggatatt attcggtggc ttgtcagcca ccaagtgagc caaggacaac
     2881 tgtgccttta ccaagggaac cttgtggtca tttgaagacc agttgcgagg agcagataag
     2941 aagaaagcca gatcagtggg cacagtacca cactcagaaa gcacctcttg tctcttcaac
     3001 tcttcctgtg gcaacacagt caccaacacc accttctcct ctgttcagtg tagactttcg
     3061 tgcggatttc tcagagagtg tgagtggtac aaaatttgaa gaagatcatc tttcccatta
     3121 ttctccctgg tcttgtggca ccataggctc ctgtataaat gccattgatt cagagcccaa
     3181 agatgtcatt gctaattcaa atgctgtgtt aatggacctg gacagtggtg atgttaagag
     3241 aagagtacat ttatttgaaa cccagagaag gacaaaagaa gaagatccaa taattccctt
     3301 tagtgatgga cccatcatct caaaatgggg tgcgatttcc agatcttccc gtacaggtta
     3361 ccataccaca gatcctgtcc aggccactgc ttcccaagga agtgcgacta agcccatcag
     3421 tgtatcagat tatgtccctt atgtcaatgc tgttgattca aggtggagtt catatggcaa
     3481 cgaggccaca tcatcagcac actatgttga aagggacaga ttcattgtta ctgatttatc
     3541 tggtcataga aagcattcca gtactgggga ccttttgagc cttgaacttc agcaggccaa
     3601 gagcaactca ttacttcttc agagagaggc caatgctttg gccatgcaac agaagtggaa
     3661 ttccctggat gaaggccgtc accttacctt aaacctttta agcaaggaaa ttgaactaag
     3721 aaatggagag ttacagagtg attatacaga agatgcaaca gatactaaac ctgataggga
     3781 tatcgagtta gagctttcag cacttgatac tgatgaacct gatggacaaa gtgaaccaat
     3841 tgaagagatc ttggacatac agcttggtat cagttctcaa aatgatcagt tgctaaatgg
     3901 aatggcagtg gaaaatgggc atccagtaca gcagcaccaa aaggagccac caaagcagaa
     3961 gaaacagagt ttaggtgaag accatgtgat tctggaggag caaaaaacaa ttctgccggt
     4021 aacttcttgc tttagccagc cactcccagt gtctattagc aatgcaagtt gcctccccat
     4081 caccacatct gtcagtgctg gcaacctcat tctgaaaact catgttatgt ctgaagataa
     4141 aaacgacttt ttaaaacctg ttgcaaatgg gaagatggtt aacagctacc cctacgacgt
     4201 gcctgactac gccggcggcg gtggttcagg cggtggcggt tccggtggtg ggggatcagt
     4261 ccaactggtg gagtctggtg gcgctttggt gcagccaggt ggctctctgc gtttgtcctg
     4321 tgccgcttct ggcttcccag tgaaccgcta ttccatgcgc tggtatcgcc aggctccagg
     4381 caaagagcgt gagtgggtag ccggtatgtc cagcgcgggt gatcgtagct cctatgaaga
     4441 ctccgtgaag ggccgtttca ccatcagccg tgacgatgcc cgtaacacgg tgtatctgca
     4501 aatgaacagc ttgaaacctg aagatacggc cgtgtattac tgtaatgtga acgtgggctt
     4561 cgagtattgg ggccaaggca cccaggtcac cgtctccagc taagatcctt gacttgcggc
     4621 cgcaactccc acctgcaaca tgcgtgactg actgaggccg cgactctaga gtcgacctgc
     4681 atctaggcgc cggaattaga tctctcgagg ttaacgaatt ctaccgggta ggggaggcgc
     4741 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     4801 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     4861 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     4921 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     4981 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     5041 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     5101 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     5161 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     5221 tttcgacctg cagcccaagc ttaccatggt gagcaagggc gaggaggata acatggccat
     5281 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     5341 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     5401 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     5461 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     5521 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     5581 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     5641 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     5701 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     5761 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     5821 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     5881 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     5941 cgagctgtac aaggcaacaa acttctctct gctgaaacaa gccggagatg tcgaagagaa
     6001 tcctggaccg atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     6061 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     6121 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     6181 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     6241 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     6301 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     6361 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     6421 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     6481 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     6541 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     6601 cggtgcctga tcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     6661 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6721 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6781 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6841 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6901 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6961 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     7021 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     7081 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     7141 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7201 cctccccgca tcgataccgt cgaccaaggc agctgtagat cttagccact ttttaaaaga
     7261 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     7321 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7381 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7441 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7501 tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg
     7561 atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat
     7621 tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga
     7681 gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca cccggaaatg
     7741 gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg
     7801 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     7861 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     7921 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     7981 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     8041 aagggagagc gtcgaatggt gcactctcag tacaatctag ctctgatgcc gcatagttaa
     8101 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     8161 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     8221 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     8281 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     8341 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8401 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     8461 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     8521 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     8581 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     8641 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     8701 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     8761 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     8821 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     8881 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     8941 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9001 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9061 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9121 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     9181 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     9241 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     9301 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     9361 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9421 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     9481 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     9541 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     9601 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     9661 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     9721 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     9781 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     9841 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     9901 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     9961 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    10021 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10081 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    10141 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    10201 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    10261 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg
    10321 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
    10381 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
    10441 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
    10501 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
    10561 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    10621 cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac
    10681 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
    10741 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
    10801 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
    10861 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
    10921 ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga
    10981 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
    11041 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
    11101 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
    11161 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
    11221 cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
    11281 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
    11341 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
    11401 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
    11461 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
    11521 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa
    11581 tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat
    11641 acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt
    11701 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
    11761 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
    11821 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
    11881 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
    11941 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
    12001 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
    12061 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
    12121 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
    12181 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
    12241 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
    12301 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
    12361 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
    12421 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    12481 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    12541 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
    12601 taccactgcc aattacctag tggtttcatt tactctaaac ctgtgattcc tctgaattat
    12661 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
    12721 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
    12781 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
    12841 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
    12901 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
    12961 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
    13021 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
    13081 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
    13141 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
    13201 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    13261 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
    13321 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
    13381 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
    13441 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
    13501 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
    13561 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
    13621 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
    13681 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
    13741 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
    13801 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
    13861 ggtgatcttc agacctggac gatatatatg agggacaatt ggagaagtga attatataaa
    13921 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
    13981 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
    14041 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
    14101 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
    14161 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
    14221 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
    14281 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
    14341 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
    14401 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
    14461 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
    14521 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
    14581 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
    14641 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
    14701 attcgattag tgaacggatc tcgacggtcg
//
