LOCUS       V033427                11991 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033427
VERSION     V033427
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..1447
                     /label="RAB40B(NM_006822)"
                     /note="RAB40B(NM_006822)"
                     /gene="RAB40B"
     CDS             1448..1474
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1520..1861
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        1987..2486
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             2507..3214
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3215..3271
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3272..3871
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    3882..4470
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             4533..4766
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(4791..4809)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        5598..5702
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             5703..6563
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      6734..7322
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        7568..7897
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9031..9096
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9226..9246
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    9671..9805
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             9974..10607
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    10654..10779
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11271..11504
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             11689..11733
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctaaggca agcgcagtga
      601 actccaagcc accatgagcg ccctgggcag cccggtccgg gcctacgact ttctgctcaa
      661 gttcctgctg gtgggcgaca gcgacgtggg caagggcgag atcctggcga gcctgcagga
      721 tggcgcggcc gagtccccgt acggccaccc ggcgggcatc gactacaaga cgaccaccat
      781 cctgctggac gggcggcggg tgaagctgca gctctgggat acttcaggcc agggaagatt
      841 ttgtaccata ttccgctcct actcccgggg cgcacagggt gtgatcctgg tctatgacat
      901 tgcgaaccgc tggtcttttg acggcattga tcgatggatt aaggagatcg atgagcatgc
      961 ccccggagtc cccaagatcc tggtggggaa ccgcctgcac ctggcgttca agcggcaggt
     1021 gcccacggag caggcccagg cctacgccga gcgcctgggc gtgaccttct ttgaggtcag
     1081 ccctctgtgc aatttcaaca tcacagagtc gttcacggag ctggccagga tcgtgctgct
     1141 gcggcatggg atggaccggc tctggcggcc gagcaaggtg ctgagcttgc aagacctctg
     1201 ctgccgggcg gtcgtgtcct gcacgccggt gcacctggtg gacaagctcc cgctccccat
     1261 tgccttaaga agccacctca agtccttctc gatggccaac ggcctgaatg ccaggatgat
     1321 gcacggcggt tcctactccc tcaccaccag ctccacccac aaaaggagca gcctccgcaa
     1381 agtgaagctc gtccgccccc cccagagccc ccccaaaaac tgcaccagaa acagctgcaa
     1441 aatttcttac ccctacgacg tgcctgacta cgccggcggc ggtggttcag gcggtggcgg
     1501 ttccggtggt gggggatcag tccaactggt ggagtctggt ggcgctttgg tgcagccagg
     1561 tggctctctg cgtttgtcct gtgccgcttc tggcttccca gtgaaccgct attccatgcg
     1621 ctggtatcgc caggctccag gcaaagagcg tgagtgggta gccggtatgt ccagcgcggg
     1681 tgatcgtagc tcctatgaag actccgtgaa gggccgtttc accatcagcc gtgacgatgc
     1741 ccgtaacacg gtgtatctgc aaatgaacag cttgaaacct gaagatacgg ccgtgtatta
     1801 ctgtaatgtg aacgtgggct tcgagtattg gggccaaggc acccaggtca ccgtctccag
     1861 ctaagatcct tgacttgcgg ccgcaactcc cacctgcaac atgcgtgact gactgaggcc
     1921 gcgactctag agtcgacctg catctaggcg ccggaattag atctctcgag gttaacgaat
     1981 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     2041 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     2101 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     2161 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     2221 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     2281 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     2341 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     2401 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     2461 cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatgg tgagcaaggg
     2521 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     2581 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     2641 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     2701 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     2761 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     2821 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     2881 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     2941 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     3001 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     3061 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     3121 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     3181 ccactccacc ggcggcatgg acgagctgta caaggcaaca aacttctctc tgctgaaaca
     3241 agccggagat gtcgaagaga atcctggacc gatgaccgag tacaagccca cggtgcgcct
     3301 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     3361 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     3421 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     3481 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     3541 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     3601 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     3661 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     3721 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     3781 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     3841 ctggtgcatg acccgcaagc ccggtgcctg atcttatcga taatcaacct ctggattaca
     3901 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     3961 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4021 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4081 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4141 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4201 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4261 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     4321 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     4381 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     4441 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgaccaagg cagctgtaga
     4501 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     4561 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     4621 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     4681 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     4741 ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt
     4801 cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac
     4861 gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct
     4921 ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac
     4981 gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc
     5041 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     5101 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     5161 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     5221 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     5281 ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatcta
     5341 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     5401 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     5461 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     5521 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     5581 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5641 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5701 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5761 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5821 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5881 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5941 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6001 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6061 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     6121 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     6181 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6241 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6301 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6361 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6421 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6481 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6541 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6601 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6661 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6721 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6781 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6841 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     6901 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6961 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7021 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7081 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7141 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7201 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7261 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7321 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7381 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7441 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7501 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     7561 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7621 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     7681 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     7741 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7801 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7861 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt
     7921 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     7981 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     8041 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     8101 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     8161 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc
     8221 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
     8281 ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga
     8341 aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga
     8401 aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt
     8461 tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg
     8521 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg
     8581 cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg
     8641 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg
     8701 aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc
     8761 ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc
     8821 gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg
     8881 aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt
     8941 atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     9001 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     9061 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
     9121 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
     9181 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
     9241 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
     9301 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
     9361 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
     9421 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
     9481 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
     9541 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
     9601 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     9661 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     9721 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     9781 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
     9841 acttcccacc ccatacccta ttaccactgc caattaccta gtggtttcat ttactctaaa
     9901 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     9961 caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg
    10021 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
    10081 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
    10141 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
    10201 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
    10261 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
    10321 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
    10381 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
    10441 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
    10501 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
    10561 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
    10621 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
    10681 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
    10741 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
    10801 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
    10861 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
    10921 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
    10981 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
    11041 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
    11101 accaccgcac agcaagcggc cggtgatctt cagacctgga cgatatatat gagggacaat
    11161 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
    11221 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
    11281 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
    11341 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
    11401 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
    11461 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
    11521 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
    11581 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
    11641 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
    11701 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
    11761 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
    11821 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
    11881 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
    11941 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggtc g
//
