LOCUS       V030109                15114 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030109
VERSION     V030109
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..4570
                     /label="NEDD4(NM_001284338)"
                     /note="NEDD4(NM_001284338)"
                     /gene="NEDD4"
     CDS             4571..4597
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4643..4984
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        5110..5609
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5630..6337
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             6338..6394
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             6395..6994
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7005..7593
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7656..7889
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(7914..7932)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        8721..8825
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             8826..9686
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      9857..10445
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        10691..11020
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          12154..12219
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             12349..12369
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    12794..12928
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             13097..13730
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    13777..13902
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    14394..14627
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             14812..14856
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctctatat attgcagtga
      601 actccaagcc accatggcac aaagcttacg attgcacttt gcagccagaa gaagcaatac
      661 ttaccctttg tcagaaacct ccggagatga cttggatagc catgttcaca tgtgcttcaa
      721 aagaccaaca cggatttcaa cgtctaacgt tgttcaaatg aagctgactc ccagacagac
      781 tgcactagct ccgttaataa aggaaaacgt tcagtctcaa gaaagatcat ctgttccctc
      841 atctgaaaat gttaataaaa agagcagctg tctacagatt tcactacagc caacaaggta
      901 cagtggatat cttcagtcta gcaatgtctt agctgatagt gatgatgctt cgtttacttg
      961 tatcttgaag gatggtattt acagtagtgc tgtggtcgat aatgaattga atgctgtgaa
     1021 tgatggtcac cttgtaagca gtccagccat ttgtagtggt agccttagta acttttcaac
     1081 cagtgataat gggtcttaca gcagcaacgg tagtgatttt gggtcatgtg caagtatcac
     1141 aagtggaggt tcatatacta acagtgtcat cagtgacagt agtagttata cttttccacc
     1201 aagtgatgat acttttttgg gtggaaactt accttctgac agcacctcca atagaagtgt
     1261 gccaaacagg aatactactc cttgtgaaat tttttcaaga agtacaagta cagatccttt
     1321 tgtccaggat gacttggaac atggattaga gattatgaaa ttgccagtga gcaggaacac
     1381 aaaaattcca ctaaaacgtt actcctcctt agtcattttt cctaggagtc cttcaactac
     1441 ccgaccgact tctccaacaa gtctgtgtac tcttctgagc aaaggatcct atcaaacttc
     1501 acaccagttt attatttctc ctagtgaaat tgcacataat gaggatggca ctagtgctaa
     1561 aggatttctt tcaacagctg tcaatggact tcggttatct aaaacaattt gtacccccgg
     1621 agaagtaaga gacatacggc cgcttcacag gaagggctcg ttacagaaga aaattgttct
     1681 ttcgaataat actcccagac agactgtctg tgaaaagtca tctgaaggat attcttgtgt
     1741 ttcagtgcat ttcacccaac gaaaagcagc tacattagac tgtgaaacaa caaatggtga
     1801 ttgtaaacca gaaatgtcag aaattaagct taattctgat tcagagtata ttaagctcat
     1861 gcataggaca tctgcatgtt tgccatcctc ccaaaatgta gattgtcaaa taaatatcaa
     1921 tggagaattg gaaagaccac attcacagat gaacaaaaac catggtattt tacgaagaag
     1981 tatttcattg ggaggagctt atccaaatat ttcctgtcta tccagcctta agcacaattg
     2041 ttctaaaggg ggaccatctc agttactcat aaagtttgca tctggaaatg aaggtaaagt
     2101 ggataattta tcaagagaca gcaacagaga ttgcacaaat gaactgtcta attcttgcaa
     2161 gacaagagat gatttcctag gtcaagtgga tgttccactt tatccattac cgacagaaaa
     2221 tccaagattg gagagaccat atacatttaa ggattttgtt cttcatccaa gaagtcacaa
     2281 atcaagagtt aaaggttatc tgagactaaa aatgacttat ttacctaaaa ccagtggctc
     2341 agaagatgat aatgcagaac aggctgagga attagagcct ggctgggttg ttttggacca
     2401 accagatgct gcttgccatt tgcagcaaca acaagaacct tctcctctac ctccagggtg
     2461 ggaagagagg caggatatcc ttggaaggac ctattatgta aaccatgaat ctagaagaac
     2521 acagtggaaa agaccaaccc ctcaggacaa cctaacagat gctgagaatg gcaacattca
     2581 actgcaagca caacgtgcat ttaccaccag gcggcagata tccgaggaaa cagaaagtgt
     2641 tgacaaccga gagtcttccg agaactggga aattataaga gaagatgaag ccaccatgta
     2701 tagcaaccag gccttcccat cacctccacc gtcaagtaac ttggatgttc caactcatct
     2761 tgcagaagaa ttgaatgcca gactcaccat ttttggaaat tcagccgtga gccagccagc
     2821 atcgagctca aatcattcca gcagaagagg cagcttacaa gcctatactt ttgaggaaca
     2881 acctacactt cctgtgcttt tgcctacttc atctggatta ccaccaggtt gggaagaaaa
     2941 acaagatgaa agaggaagat catattatgt agatcacaat tccagaacga ctacttggac
     3001 aaagcccact gtacaggcca cagtggagac cagtcagctg acctcaagcc agagttctgc
     3061 aggccctcaa tcacaagcct ccaccagtga ttcaggccag caggtgaccc agccatctga
     3121 aattgagcaa ggattccttc ctaaaggctg ggaagtccgg catgcaccaa atgggaggcc
     3181 tttctttatt gaccacaaca ctaaaaccac cacctgggaa gatccaagat tgaaaattcc
     3241 agcccatctg agaggaaaga catcacttga tacttccaat gatctagggc ctttacctcc
     3301 aggatgggaa gagagaactc acacagatgg aagaatcttc tacataaatc acaatataaa
     3361 aagaacacaa tgggaagatc ctcggttgga gaatgtagca ataactggac cagcagtgcc
     3421 ctactccagg gattacaaaa gaaagtatga gttcttccga agaaagttga agaagcagaa
     3481 tgacattcca aacaaatttg aaatgaaact tcgccgagca actgttcttg aagactctta
     3541 ccggagaatt atgggtgtca agagagcaga cttcctgaag gctcgactgt ggattgagtt
     3601 tgatggtgaa aagggattgg attatggagg agttgccaga gaatggttct tcctgatctc
     3661 aaaggaaatg tttaaccctt attatgggtt gtttgaatat tctgctacgg acaattatac
     3721 cctacagata aatccaaact ctggattgtg taacgaagat cacctctctt acttcaagtt
     3781 tattggtcgg gtagctggaa tggcagttta tcatggcaaa ctgttggatg gttttttcat
     3841 ccgcccattt tacaagatga tgcttcacaa accaataacc cttcatgata tggaatctgt
     3901 ggatagtgaa tattacaatt ccctaagatg gattcttgaa aatgacccaa cagaattgga
     3961 cctcaggttt atcatagatg aagaactttt tggacagaca catcaacatg agctgaaaaa
     4021 tggtggatca gaaatagttg tcaccaataa gaacaaaaag gaatatattt atcttgtaat
     4081 acaatggcga tttgtaaacc gaatccagaa gcaaatggct gcttttaaag agggattctt
     4141 tgaactaata ccacaggatc tcatcaaaat ttttgatgaa aatgaactag agcttcttat
     4201 gtgtggactg ggagatgttg atgtgaatga ctggagggaa catacaaagt ataaaaatgg
     4261 ctacagtgca aatcatcagg ttatacagtg gttttggaag gctgttttaa tgatggattc
     4321 agaaaaaaga ataagattac ttcagtttgt cactggcaca tctcgggtgc ctatgaatgg
     4381 atttgctgaa ctatacggtt caaatggacc acagtcattt acagttgaac agtggggtac
     4441 tcctgaaaag ctgccaagag ctcatacctg ttttaatcgc ctggacttgc caccttatga
     4501 atcatttgaa gaattatggg ataaacttca gatggcaatt gaaaacaccc agggctttga
     4561 tggagttgat tacccctacg acgtgcctga ctacgccggc ggcggtggtt caggcggtgg
     4621 cggttccggt ggtgggggat cagtccaact ggtggagtct ggtggcgctt tggtgcagcc
     4681 aggtggctct ctgcgtttgt cctgtgccgc ttctggcttc ccagtgaacc gctattccat
     4741 gcgctggtat cgccaggctc caggcaaaga gcgtgagtgg gtagccggta tgtccagcgc
     4801 gggtgatcgt agctcctatg aagactccgt gaagggccgt ttcaccatca gccgtgacga
     4861 tgcccgtaac acggtgtatc tgcaaatgaa cagcttgaaa cctgaagata cggccgtgta
     4921 ttactgtaat gtgaacgtgg gcttcgagta ttggggccaa ggcacccagg tcaccgtctc
     4981 cagctaagat ccttgacttg cggccgcaac tcccacctgc aacatgcgtg actgactgag
     5041 gccgcgactc tagagtcgac ctgcatctag gcgccggaat tagatctctc gaggttaacg
     5101 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     5161 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     5221 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     5281 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     5341 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     5401 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     5461 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     5521 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     5581 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca tggtgagcaa
     5641 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     5701 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     5761 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     5821 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     5881 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     5941 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     6001 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     6061 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     6121 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     6181 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     6241 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     6301 ccgccactcc accggcggca tggacgagct gtacaaggca acaaacttct ctctgctgaa
     6361 acaagccgga gatgtcgaag agaatcctgg accgatgacc gagtacaagc ccacggtgcg
     6421 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     6481 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     6541 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     6601 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     6661 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     6721 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     6781 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     6841 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     6901 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     6961 cacctggtgc atgacccgca agcccggtgc ctgatcttat cgataatcaa cctctggatt
     7021 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     7081 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     7141 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     7201 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     7261 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     7321 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     7381 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     7441 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     7501 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     7561 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacca aggcagctgt
     7621 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7681 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7741 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7801 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7861 cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag
     7921 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
     7981 aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca
     8041 tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt
     8101 aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag
     8161 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
     8221 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
     8281 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
     8341 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
     8401 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
     8461 ctagctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     8521 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     8581 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     8641 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     8701 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     8761 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     8821 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     8881 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     8941 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     9001 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     9061 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     9121 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     9181 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     9241 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     9301 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9361 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9421 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9481 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     9541 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     9601 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     9661 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     9721 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9781 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9841 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9901 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     9961 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
    10021 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    10081 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    10141 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    10201 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    10261 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    10321 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    10381 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10441 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10501 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    10561 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    10621 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    10681 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
    10741 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
    10801 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
    10861 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
    10921 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
    10981 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg
    11041 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
    11101 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
    11161 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
    11221 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
    11281 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt
    11341 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
    11401 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
    11461 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
    11521 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
    11581 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
    11641 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
    11701 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
    11761 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
    11821 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
    11881 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
    11941 ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
    12001 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga
    12061 tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
    12121 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
    12181 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
    12241 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
    12301 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
    12361 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
    12421 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
    12481 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
    12541 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
    12601 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
    12661 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
    12721 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
    12781 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    12841 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    12901 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
    12961 caaacttccc accccatacc ctattaccac tgccaattac ctagtggttt catttactct
    13021 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
    13081 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
    13141 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
    13201 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
    13261 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
    13321 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
    13381 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
    13441 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
    13501 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
    13561 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
    13621 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    13681 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
    13741 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
    13801 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
    13861 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
    13921 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
    13981 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
    14041 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
    14101 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
    14161 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
    14221 aagaccaccg cacagcaagc ggccggtgat cttcagacct ggacgatata tatgagggac
    14281 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
    14341 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
    14401 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
    14461 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
    14521 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
    14581 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
    14641 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
    14701 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
    14761 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
    14821 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
    14881 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
    14941 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
    15001 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
    15061 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtcg
//
