LOCUS       V033603                13677 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033603
VERSION     V033603
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..3133
                     /label="LZTR1(NM_006767)"
                     /note="LZTR1(NM_006767)"
                     /gene="LZTR1"
     CDS             3134..3160
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3206..3547
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3673..4172
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4193..4900
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4901..4957
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4958..5557
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5568..6156
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6219..6452
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6477..6495)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7284..7388
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7389..8249
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8420..9008
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9254..9583
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10717..10782
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10912..10932
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11357..11491
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11660..12293
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12340..12465
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12957..13190
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13375..13419
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctattgtt ggagcagtga
      601 actccaagcc accatggctg gaccgggcag cacggggggg cagatcgggg ctgcggccct
      661 ggcaggcggc gcgcggtcca aggtagcccc gagcgtggac ttcgaccata gctgctcgga
      721 cagtgtcgag tacctgacgc tcaacttcgg gcccttcgaa acagtgcatc gctggcggcg
      781 cctcccgccc tgcgacgagt tcgtgggtgc ccggcgcagc aagcacacag tggtggccta
      841 taaagatgcc atttatgtat ttggtggaga caatgggaag accatgctca atgacctcct
      901 gcggttcgat gtgaaagact gctcctggtg cagggccttt accactggga ccccaccggc
      961 cccccgttac caccactcgg ccgtcgtcta tgggagcagc atgtttgtct ttgggggtta
     1021 cactggggac atttattcca attctaactt gaagaataaa aacgacctct ttgaatacaa
     1081 gtttgcaact ggccagtgga cggagtggaa aattgaagga cggttgccag tcgctaggtc
     1141 agcccatggg gccacggtgt acagtgacaa gctgtggatc tttgctggct atgacggcaa
     1201 cgccaggttg aatgacatgt ggacaattgg cctccaggac cgagagctca cctgctggga
     1261 ggaggtggcc cagagtggcg agatcccccc atcttgctgc aacttccccg tggctgtgtg
     1321 ccgggacaag atgtttgtat tctctgggca aagcggagcc aaaataacca acaacctctt
     1381 ccagtttgaa ttcaaggaca agacgtggac acgcatccca actgaacacc tgctccgggg
     1441 ctccccacca cccccgcagc ggcgctacgg gcataccatg gtggcctttg accgccacct
     1501 ctatgtgttt gggggtgcgg ccgacaacac gctgcccaac gagctgcact gctatgacgt
     1561 ggacttccag acctgggagg tcgtccagcc cagctccgac agcgaggttg gtggggctga
     1621 agtgcccgag cgagcctgtg cttccgagga ggtgcccacc ctgacctatg aggagcgggt
     1681 tggcttcaag aagtcccgag atgtgtttgg cctggacttt ggcaccacct cagccaagca
     1741 gcccacccag cctgcctcgg agctgcccag tgggaggctc ttccacgcgg ctgctgtcat
     1801 ctcggacgcc atgtacatct tcgggggcac ggtggacaac aacatccgca gcggggagat
     1861 gtacaggttc cagttctcct gttaccctaa atgcacgctg cacgaggact acgggcggct
     1921 gtgggagagc cgccagttct gcgacgtgga gttcgtgctg ggtgagaagg aggagtgcgt
     1981 gcagggccac gtagccattg tcacagcgcg gagccgctgg cttcgcagga agatcacgca
     2041 ggcgcgggag aggctggccc agaagctgga gcaggaggcc gccccagttc ccagggaggc
     2101 ccccggcgtg gctgctggtg gggcccggcc gcccctgctg cacgtggcca tccgggaggc
     2161 cgaggcccgg cccttcgagg tgctcatgca gttcctctac accgacaaga tcaaataccc
     2221 acggaaaggc catgtggagg atgtgctgct catcatggat gtgtacaaac tggcactgag
     2281 cttccagttg tgccgcctgg agcagctgtg ccgccagtac atcgaggcct ccgtggacct
     2341 gcagaacgtg ctggttgtgt gcgagagtgc cgcccggctg cagctgagcc aactcaagga
     2401 gcactgcctg aacttcgtgg taaaggagtc ccacttcaac caggtgatca tgatgaagga
     2461 gttcgagcgc ctctcctctc cactgatagt ggagattgtg cggcggaagc agcagccgcc
     2521 ccctcgcact cccttggacc agccagtgga cattggcaca tctctgatcc aggacatgaa
     2581 ggcatacctg gagggagcgg gcgcggaatt ctgtgacatc actctgttgc ttgacgggca
     2641 cccacggcca gcccacaagg ctatcctggc cgcccgctcc agctactttg aagccatgtt
     2701 ccggtccttc atgcccgaag atgggcaggt gaacatctcc atcggggaga tggtgcccag
     2761 caggcaggcc ttcgagtcca tgctgcgcta catctactac ggcgaggtca acatgccgcc
     2821 cgaggactcg ctctacttgt ttgcggcccc ctactactac ggcttctaca acaaccggct
     2881 gcaggcgtac tgcaagcaga acctggagat gaacgtgacg gtgcagaacg tgctgcagat
     2941 cctggaggca gctgacaaaa cgcaggcact ggacatgaag cggcactgcc tgcacatcat
     3001 tgtgcaccag ttcaccaagg tctccaagtt gcccaccctg cggtcgctga gccagcagct
     3061 gctgctggac atcatagact ccctggcctc ccacatctca gacaagcagt gcgcagagct
     3121 gggcgccgac atctacccct acgacgtgcc tgactacgcc ggcggcggtg gttcaggcgg
     3181 tggcggttcc ggtggtgggg gatcagtcca actggtggag tctggtggcg ctttggtgca
     3241 gccaggtggc tctctgcgtt tgtcctgtgc cgcttctggc ttcccagtga accgctattc
     3301 catgcgctgg tatcgccagg ctccaggcaa agagcgtgag tgggtagccg gtatgtccag
     3361 cgcgggtgat cgtagctcct atgaagactc cgtgaagggc cgtttcacca tcagccgtga
     3421 cgatgcccgt aacacggtgt atctgcaaat gaacagcttg aaacctgaag atacggccgt
     3481 gtattactgt aatgtgaacg tgggcttcga gtattggggc caaggcaccc aggtcaccgt
     3541 ctccagctaa gatccttgac ttgcggccgc aactcccacc tgcaacatgc gtgactgact
     3601 gaggccgcga ctctagagtc gacctgcatc taggcgccgg aattagatct ctcgaggtta
     3661 acgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     3721 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     3781 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     3841 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     3901 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     3961 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     4021 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     4081 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     4141 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatggtgag
     4201 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     4261 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     4321 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     4381 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     4441 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     4501 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     4561 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     4621 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     4681 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     4741 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     4801 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     4861 gggccgccac tccaccggcg gcatggacga gctgtacaag gcaacaaact tctctctgct
     4921 gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg accgagtaca agcccacggt
     4981 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     5041 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     5101 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     5161 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     5221 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     5281 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     5341 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     5401 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     5461 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     5521 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgatct tatcgataat caacctctgg
     5581 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5641 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5701 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5761 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5821 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5881 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5941 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     6001 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6061 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     6121 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga ccaaggcagc
     6181 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6241 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     6301 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6361 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6421 acccttttag tcagtgtgga aaatctctag cagcatctag aattaattcc gtgtattcta
     6481 tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt
     6541 tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta
     6601 tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct
     6661 ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc
     6721 cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
     6781 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
     6841 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
     6901 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
     6961 ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac
     7021 aatctagctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     7081 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     7141 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     7201 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     7261 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     7321 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     7381 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     7441 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     7501 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     7561 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     7621 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7681 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7741 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7801 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7861 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7921 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7981 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     8041 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     8101 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     8161 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     8221 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     8281 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     8341 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8401 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8461 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8521 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     8581 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8641 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8701 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8761 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8821 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8881 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8941 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     9001 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     9061 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     9121 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     9181 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     9241 gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     9301 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     9361 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     9421 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     9481 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     9541 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac
     9601 aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
     9661 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg
     9721 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
     9781 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
     9841 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
     9901 tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat
     9961 ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga
    10021 tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat
    10081 tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc
    10141 gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga
    10201 tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact
    10261 gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg
    10321 aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac
    10381 atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt
    10441 cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga
    10501 tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg
    10561 atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact
    10621 ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa
    10681 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
    10741 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
    10801 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
    10861 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
    10921 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
    10981 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
    11041 gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac
    11101 agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt
    11161 aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag
    11221 gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga
    11281 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
    11341 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    11401 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    11461 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
    11521 tcccaaactt cccaccccat accctattac cactgccaat tacctagtgg tttcatttac
    11581 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
    11641 gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt
    11701 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
    11761 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
    11821 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
    11881 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
    11941 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
    12001 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
    12061 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
    12121 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    12181 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    12241 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
    12301 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
    12361 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
    12421 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
    12481 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
    12541 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
    12601 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
    12661 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
    12721 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
    12781 agtaagacca ccgcacagca agcggccggt gatcttcaga cctggacgat atatatgagg
    12841 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
    12901 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
    12961 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
    13021 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
    13081 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
    13141 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
    13201 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
    13261 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
    13321 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
    13381 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
    13441 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
    13501 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
    13561 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
    13621 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtcg
//
