LOCUS       V030158                13341 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030158
VERSION     V030158
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2797
                     /label="LNX1(NM_001126328)"
                     /note="LNX1(NM_001126328)"
                     /gene="LNX1"
     CDS             2798..2824
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2870..3211
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3337..3836
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3857..4564
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4565..4621
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4622..5221
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5232..5820
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5883..6116
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6141..6159)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6948..7052
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7053..7913
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8084..8672
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8918..9247
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10381..10446
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10576..10596
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11021..11155
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11324..11957
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12004..12129
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12621..12854
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13039..13083
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctcgaaaa tttgcagtga
      601 actccaagcc accatgaacc agccagagtc tgccaacgat cctgaacccc tgtgtgcagt
      661 gtgtggccaa gcccactcct tggaggaaaa ccacttctac agctatccag aggaagtgga
      721 tgatgacctc atctgccaca tctgcctgca ggctttgctg gaccccctgg acactccgtg
      781 tggacacacc tactgcaccc tctgcctcac caacttcctg gtggagaagg acttctgtcc
      841 catggaccgc aagcctctgg ttctgcagca ctgcaagaag tccagcatcc tggtcaacaa
      901 actcctcaac aagctactgg tgacctgccc attcagggag cactgcaccc aggtgttgca
      961 gcgctgtgac ctcgagcatc actttcaaac cagctgtaaa ggtgcctccc actacggcct
     1021 gaccaaagat aggaagaggc gctcacaaga tggctgtcca gacggctgtg cgagcctcac
     1081 agccacggct ccctccccag aggtttctgc agctgccacc atctccttaa tgacagacga
     1141 gcctggccta gacaaccctg cctacgtgtc ctcggcagag gacgggcagc cagcaatcag
     1201 cccagtggac tctggccgga gcaaccgaac tagggcacgg ccctttgaga gatccactat
     1261 tagaagcaga tcatttaaaa aaataaatcg agctttgagt gttcttcgaa ggacaaagag
     1321 cgggagtgca gttgccaacc atgccgacca gggcagggaa aattctgaaa acaccactgc
     1381 ccctgaagtc tttccaaggt tgtaccacct gattccagat ggtgaaatta ccagcatcaa
     1441 gatcaatcga gtagatccca gtgaaagcct ctctattagg ctggtgggag gtagcgaaac
     1501 cccactggtc catatcatta tccaacacat ttatcgtgat ggggtgatcg ccagagacgg
     1561 ccggctactg ccaggagaca tcattctaaa ggtcaacggg atggacatca gcaatgtccc
     1621 tcacaactac gctgtgcgtc tcctgcggca gccctgccag gtgctgtggc tgactgtgat
     1681 gcgtgaacag aagttccgca gcaggaacaa tggacaggcc ccggatgcct acagaccccg
     1741 agatgacagc tttcatgtga ttctcaacaa aagtagcccc gaggagcagc ttggaataaa
     1801 actggtgcgc aaggtggatg agcctggggt tttcatcttc aatgtgctgg atggcggtgt
     1861 ggcatatcga catggtcagc ttgaggagaa tgaccgtgtg ttagccatca atggacatga
     1921 tcttcgatat ggcagcccag aaagtgcggc tcatctgatt caggccagtg aaagacgtgt
     1981 tcacctcgtc gtgtcccgcc aggttcggca gcggagccct gacatctttc aggaagccgg
     2041 ctggaacagc aatggcagct ggtccccagg gccaggggag aggagcaaca ctcccaagcc
     2101 cctccatcct acaattactt gtcatgagaa ggtggtaaat atccaaaaag accccggtga
     2161 atctctcggc atgaccgtcg cagggggagc atcacataga gaatgggatt tgcctatcta
     2221 tgtcatcagt gttgagcccg gaggagtcat aagcagagat ggaagaataa aaacaggtga
     2281 cattttgttg aatgtggatg gggtcgaact gacagaggtc agccggagtg aggcagtggc
     2341 attattgaaa agaacatcat cctcgatagt actcaaagct ttggaagtca aagagtatga
     2401 gccccaggaa gactgcagca gcccagcagc cctggactcc aaccacaaca tggccccacc
     2461 cagtgactgg tccccatcct gggtcatgtg gctggaatta ccacggtgct tgtataactg
     2521 taaagatatt gtattacgaa gaaacacagc tggaagtctg ggcttctgca ttgtaggagg
     2581 ttatgaagaa tacaatggaa acaaaccttt tttcatcaaa tccattgttg aaggaacacc
     2641 agcatacaat gatggaagaa ttagatgtgg tgatattctt cttgctgtca atggtagaag
     2701 tacatcagga atgatacatg cttgcttggc aagactgctg aaagaactta aaggaagaat
     2761 tactctaact attgtttctt ggcctggcac ttttttatac ccctacgacg tgcctgacta
     2821 cgccggcggc ggtggttcag gcggtggcgg ttccggtggt gggggatcag tccaactggt
     2881 ggagtctggt ggcgctttgg tgcagccagg tggctctctg cgtttgtcct gtgccgcttc
     2941 tggcttccca gtgaaccgct attccatgcg ctggtatcgc caggctccag gcaaagagcg
     3001 tgagtgggta gccggtatgt ccagcgcggg tgatcgtagc tcctatgaag actccgtgaa
     3061 gggccgtttc accatcagcc gtgacgatgc ccgtaacacg gtgtatctgc aaatgaacag
     3121 cttgaaacct gaagatacgg ccgtgtatta ctgtaatgtg aacgtgggct tcgagtattg
     3181 gggccaaggc acccaggtca ccgtctccag ctaagatcct tgacttgcgg ccgcaactcc
     3241 cacctgcaac atgcgtgact gactgaggcc gcgactctag agtcgacctg catctaggcg
     3301 ccggaattag atctctcgag gttaacgaat tctaccgggt aggggaggcg cttttcccaa
     3361 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
     3421 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
     3481 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
     3541 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
     3601 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
     3661 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
     3721 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
     3781 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
     3841 gcagcccaag cttaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     3901 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     3961 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     4021 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     4081 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     4141 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     4201 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     4261 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     4321 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     4381 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     4441 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     4501 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     4561 caaggcaaca aacttctctc tgctgaaaca agccggagat gtcgaagaga atcctggacc
     4621 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     4681 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     4741 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     4801 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     4861 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     4921 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     4981 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     5041 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     5101 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     5161 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     5221 atcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5281 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5341 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5401 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     5461 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     5521 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     5581 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     5641 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     5701 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     5761 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     5821 atcgataccg tcgaccaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5881 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     5941 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6001 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6061 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     6121 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
     6181 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
     6241 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
     6301 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
     6361 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     6421 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     6481 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     6541 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     6601 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     6661 cgtcgaatgg tgcactctca gtacaatcta gctctgatgc cgcatagtta agccagcccc
     6721 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     6781 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     6841 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     6901 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6961 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7021 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7081 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7141 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7201 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7261 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7321 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7381 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7441 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7501 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7561 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7621 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7681 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7741 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7801 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7861 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7921 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7981 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8041 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8101 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8161 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8221 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8281 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8341 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8401 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8461 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8521 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8581 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8641 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8701 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8761 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8821 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8881 ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa
     8941 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     9001 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     9061 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     9121 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9181 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     9241 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc
     9301 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
     9361 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
     9421 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
     9481 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
     9541 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat
     9601 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
     9661 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
     9721 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
     9781 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
     9841 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
     9901 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
     9961 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
    10021 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
    10081 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc
    10141 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg
    10201 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg
    10261 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa
    10321 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
    10381 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
    10441 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
    10501 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
    10561 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
    10621 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
    10681 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
    10741 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
    10801 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
    10861 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
    10921 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
    10981 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
    11041 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    11101 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
    11161 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
    11221 caattaccta gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    11281 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac
    11341 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
    11401 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
    11461 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
    11521 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
    11581 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
    11641 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
    11701 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
    11761 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
    11821 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
    11881 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
    11941 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
    12001 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
    12061 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
    12121 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
    12181 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
    12241 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
    12301 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
    12361 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
    12421 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggtgatctt
    12481 cagacctgga cgatatatat gagggacaat tggagaagtg aattatataa atataaagta
    12541 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
    12601 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
    12661 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
    12721 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
    12781 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
    12841 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
    12901 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
    12961 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
    13021 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
    13081 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
    13141 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
    13201 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
    13261 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
    13321 gtgaacggat ctcgacggtc g
//
