LOCUS       V033237                13017 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033237
VERSION     V033237
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2473
                     /label="KLHL8(NM_001292003)"
                     /note="KLHL8(NM_001292003)"
                     /gene="KLHL8"
     CDS             2474..2500
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2546..2887
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3013..3512
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3533..4240
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4241..4297
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4298..4897
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4908..5496
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5559..5792
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5817..5835)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6624..6728
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6729..7589
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7760..8348
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8594..8923
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10057..10122
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10252..10272
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10697..10831
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11000..11633
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11680..11805
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12297..12530
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12715..12759
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctgtatat tgtgcagtga
      601 actccaagcc accatggctt cagattctat gagtagtaaa caagctagga atcacattac
      661 aaaggggaaa aggcaacaac agcaccagca aataaagaac agatcctcaa ttagtgatgg
      721 tgatggagaa gattccttta tttttgaagc aaatgaagct tggaaagatt ttcatggttc
      781 tcttcttcga ttttatgaaa atggagaact ctgtgatgtc acactcaagg ttggctcaaa
      841 gctaatctct tgtcacaagc tggtattggc ttgtgttatt ccctacttta gagccatgtt
      901 tctttctgaa atggctgaag ccaagcaaac gctgattgag attagagatt ttgatggtga
      961 tgcaatagaa gacttggtaa agtttgtcta ttcttcacgg ctcactttga ctgttgacaa
     1021 tgtccagcct ctcttatatg cagcctgtat tctgcaggtt gaactggtgg ctagagcttg
     1081 ttgtgaatac atgaagttac attttcatcc ctccaattgc ctggcagtaa gagcctttgc
     1141 agaaagtcac aatcgaatag acttaatgga catggcggat cagtatgcct gtgaccattt
     1201 tactgaagta gtggagtgtg aagactttgt aagtgtatca ccgcagcacc tccataagct
     1261 tttgtcctcc agtgatctaa atattgaaaa tgaaaagcag gtctataatg ctgccatcaa
     1321 gtggcttctt gccaatcctc agcatcattc caaatggttg gatgaaacac ttgcacaggt
     1381 tcgcctgcca ttgttgccgg ttgattttct tatgggtgtt gtggcaaaag aacagattgt
     1441 caagcaaaat ctaaaatgta gagatttact ggatgaagca agaaattacc accttcactt
     1501 gagtagcaga gcagtacctg actttgaata ctccattcgg actaccccaa ggaagcatac
     1561 tgctggtgtg ctgttttgtg taggtggtcg aggtggatct ggtgacccct ttcgcagtat
     1621 tgaatgctat tctatcaaca aaaacagttg gttctttgga ccagaaatga atagtcgaag
     1681 gcgacatgtg ggtgtaatct ctgtggaagg taaagtgtat gcagtaggtg gacatgatgg
     1741 aaatgaacat ttagggagta tggagatgtt tgatcctctc actaataaat ggatgatgaa
     1801 ggcatcaatg aacacaaaga ggcgaggaat tgccttggct tccttaggag gcccaattta
     1861 tgcaattgga gggttagatg acaatacttg cttcaatgat gtggagagat atgacataga
     1921 atctgatcag tggagtacag tggcaccaat gaatactccc cgtggaggag ttggctctgt
     1981 tgctctagta aaccatgttt atgcagtagg tggcaatgat ggaatggctt ctttatctag
     2041 cgtggagaga tatgatccac atctggataa gtggatagaa gttaaagaaa tgggtcagcg
     2101 aagagcaggc aatggagtta gcaagcttca tggttgctta tacgtagttg gtggttttga
     2161 tgataattct cctctgagtt cagttgagcg gtatgacccc cgaagcaaca agtgggatta
     2221 tgtggcagca cttactactc ccagaggtgg agtgggaatc gcaacagtga tgggcaaaat
     2281 ctttgcagtt ggtggtcata atggcaatgc atacttaaat acagtagaag cgtttgatcc
     2341 agtgctgaat aggtgggagc ttgttggatc tgtgtctcac tgcagagctg gagcaggagt
     2401 agctgtgtgt tcctgtttaa ctagccaaat tcgagatgta ggtcatggat ccaataatgt
     2461 ggttgactgt atgtacccct acgacgtgcc tgactacgcc ggcggcggtg gttcaggcgg
     2521 tggcggttcc ggtggtgggg gatcagtcca actggtggag tctggtggcg ctttggtgca
     2581 gccaggtggc tctctgcgtt tgtcctgtgc cgcttctggc ttcccagtga accgctattc
     2641 catgcgctgg tatcgccagg ctccaggcaa agagcgtgag tgggtagccg gtatgtccag
     2701 cgcgggtgat cgtagctcct atgaagactc cgtgaagggc cgtttcacca tcagccgtga
     2761 cgatgcccgt aacacggtgt atctgcaaat gaacagcttg aaacctgaag atacggccgt
     2821 gtattactgt aatgtgaacg tgggcttcga gtattggggc caaggcaccc aggtcaccgt
     2881 ctccagctaa gatccttgac ttgcggccgc aactcccacc tgcaacatgc gtgactgact
     2941 gaggccgcga ctctagagtc gacctgcatc taggcgccgg aattagatct ctcgaggtta
     3001 acgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     3061 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     3121 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     3181 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     3241 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     3301 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     3361 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     3421 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     3481 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatggtgag
     3541 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     3601 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     3661 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     3721 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     3781 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     3841 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     3901 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     3961 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     4021 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     4081 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     4141 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     4201 gggccgccac tccaccggcg gcatggacga gctgtacaag gcaacaaact tctctctgct
     4261 gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg accgagtaca agcccacggt
     4321 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     4381 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     4441 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     4501 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     4561 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     4621 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     4681 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     4741 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     4801 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     4861 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgatct tatcgataat caacctctgg
     4921 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     4981 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5041 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5101 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5161 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5221 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5281 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     5341 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     5401 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     5461 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga ccaaggcagc
     5521 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     5581 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     5641 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     5701 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     5761 acccttttag tcagtgtgga aaatctctag cagcatctag aattaattcc gtgtattcta
     5821 tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt
     5881 tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta
     5941 tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct
     6001 ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc
     6061 cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
     6121 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
     6181 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
     6241 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
     6301 ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac
     6361 aatctagctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     6421 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     6481 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     6541 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     6601 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6661 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6721 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6781 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6841 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6901 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6961 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7021 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7081 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7141 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7201 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7261 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7321 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7381 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7441 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7501 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7561 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7621 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7681 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7741 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7801 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7861 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     7921 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7981 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8041 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8101 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8161 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8221 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8281 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8341 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8401 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8461 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     8521 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     8581 gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     8641 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     8701 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     8761 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8821 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     8881 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac
     8941 aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
     9001 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg
     9061 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
     9121 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
     9181 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
     9241 tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat
     9301 ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga
     9361 tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat
     9421 tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc
     9481 gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga
     9541 tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact
     9601 gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg
     9661 aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac
     9721 atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt
     9781 cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga
     9841 tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg
     9901 atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact
     9961 ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa
    10021 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
    10081 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
    10141 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
    10201 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
    10261 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
    10321 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
    10381 gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac
    10441 agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt
    10501 aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag
    10561 gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga
    10621 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
    10681 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    10741 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    10801 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
    10861 tcccaaactt cccaccccat accctattac cactgccaat tacctagtgg tttcatttac
    10921 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
    10981 gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt
    11041 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
    11101 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
    11161 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
    11221 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
    11281 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
    11341 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
    11401 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
    11461 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    11521 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    11581 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
    11641 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
    11701 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
    11761 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
    11821 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
    11881 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
    11941 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
    12001 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
    12061 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
    12121 agtaagacca ccgcacagca agcggccggt gatcttcaga cctggacgat atatatgagg
    12181 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
    12241 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
    12301 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
    12361 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
    12421 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
    12481 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
    12541 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
    12601 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
    12661 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
    12721 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
    12781 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
    12841 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
    12901 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
    12961 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtcg
//
