LOCUS       V033381                13005 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033381
VERSION     V033381
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2461
                     /label="KLHL36(NM_024731)"
                     /note="KLHL36(NM_024731)"
                     /gene="KLHL36"
     CDS             2462..2488
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2534..2875
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3001..3500
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3521..4228
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4229..4285
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4286..4885
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4896..5484
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5547..5780
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5805..5823)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6612..6716
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6717..7577
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7748..8336
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8582..8911
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10045..10110
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10240..10260
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10685..10819
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10988..11621
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11668..11793
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12285..12518
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12703..12747
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctagttgt tgcgcagtga
      601 actccaagcc accatgatgg agggaagcag gcagacgcga gtgtctcggc catacaagat
      661 cagcgaatca tcaaaggtat accgctgggc cgaccactca agcacggtgc tgcagcggct
      721 gaacgagcag cgtctccgcg ggctcttctg cgacgtcgtc ctggtggccg atgagcagcg
      781 tgtgccagcc catcgcaacc tgctggccgt gtgcagcgac tacttcaact ccatgttcac
      841 catcggcatg cgggaagctt tccagaagga ggtggagctg atcggcgcct cctacattgg
      901 gctcaaggcc gtggtggact tcctgtacgg cggggagctg gtgctggatg gcggcaacat
      961 tgactacgtc ctggagacgg ctcacctgct gcagatctgg acggtggtag acttctgctg
     1021 tgagtacctg gagcaggagg tgagcgagga caactacctg tacctgcagg agctggcctc
     1081 catctacagc ctcaagcggc ttgatgcctt catcgatggc ttcatcctga accacttcgg
     1141 cacgctgtcc tttacgcccg acttcctgca gaacgtctcc atgcagaagc tgtgtgtcta
     1201 cctgagcagc agcgaggtgc agcgggagtg tgagcacgac ctcctgcagg ccgccctgca
     1261 gtggctgacg cagcagcccg agcgcgaggc ccacgcccgc caggtgctgg agaacatcca
     1321 cttcccgctc atccccaaga acgacctgct gcaccgcgtc aagccggccg tgtgctcgct
     1381 gctgcccaag gaggccaact gcgagggctt catcgaggag gccgtgcgct accacaacaa
     1441 cctggcggcc cagcccgtca tgcagaccaa gcgcacggcg ctgcgcacca accaggagcg
     1501 cctgctgttt gtgggcggcg aggtctccga gcggtgtctg gagctcagtg acgacacctg
     1561 ctacctggac gccaagagcg agcagtgggt caaagagacg ccgctgcccg cccggcggag
     1621 ccaccactgt gtcgcggtgc tggggggctt catcttcatc gccggcggca gcttctcacg
     1681 ggacaacgga ggggatgcgg cctccaatct tctttatagg tatgaccccc gctgtaaaca
     1741 gtggatcaag gtggcctcca tgaaccagcg ccgtgtggat ttctaccttg cctccatcga
     1801 agacatgctg gtggccatcg gcggccggaa tgagaacgga gcgctctctt cagtagagac
     1861 gtacagtccc aagactgact cctggtccta tgtggccggc ttgccaaggt tcacgtacgg
     1921 ccacgcgggc accatctaca aagacttcgt gtacatctcg gggggccacg actaccaaat
     1981 tggcccctac cgcaagaacc tgctatgcta cgaccaccgg acagacgtgt gggaggagcg
     2041 gcggcccatg accacggcgc gcggctggca cagcatgtgc agcctgggtg acagcatcta
     2101 ctccatcggg ggcagcgatg acaacatcga gtccatggag cgcttcgacg tgctgggcgt
     2161 ggaggcctac agcccgcagt gcaaccagtg gacccgcgtg gcgccgctgc tgcacgccaa
     2221 cagcgagtcg ggcgtggcag tgtgggaggg ccgcatctac atcctgggcg gctacagctg
     2281 ggagaacact gccttctcca agaccgtgca ggtgtacgac cgcgaggccg acaagtggag
     2341 caggggcgtc gacctgccca aggccatcgc tggcgggtcc gcctgtgtct gcgccctgga
     2401 gccacggcca gaggacaaga agaagaaagg caaaggcaag aggcaccagg accggggcca
     2461 gtacccctac gacgtgcctg actacgccgg cggcggtggt tcaggcggtg gcggttccgg
     2521 tggtggggga tcagtccaac tggtggagtc tggtggcgct ttggtgcagc caggtggctc
     2581 tctgcgtttg tcctgtgccg cttctggctt cccagtgaac cgctattcca tgcgctggta
     2641 tcgccaggct ccaggcaaag agcgtgagtg ggtagccggt atgtccagcg cgggtgatcg
     2701 tagctcctat gaagactccg tgaagggccg tttcaccatc agccgtgacg atgcccgtaa
     2761 cacggtgtat ctgcaaatga acagcttgaa acctgaagat acggccgtgt attactgtaa
     2821 tgtgaacgtg ggcttcgagt attggggcca aggcacccag gtcaccgtct ccagctaaga
     2881 tccttgactt gcggccgcaa ctcccacctg caacatgcgt gactgactga ggccgcgact
     2941 ctagagtcga cctgcatcta ggcgccggaa ttagatctct cgaggttaac gaattctacc
     3001 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     3061 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     3121 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     3181 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     3241 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     3301 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     3361 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     3421 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     3481 cctcatctcc gggcctttcg acctgcagcc caagcttacc atggtgagca agggcgagga
     3541 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     3601 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     3661 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     3721 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     3781 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     3841 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     3901 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     3961 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     4021 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     4081 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     4141 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     4201 caccggcggc atggacgagc tgtacaaggc aacaaacttc tctctgctga aacaagccgg
     4261 agatgtcgaa gagaatcctg gaccgatgac cgagtacaag cccacggtgc gcctcgccac
     4321 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     4381 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     4441 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     4501 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     4561 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     4621 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     4681 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     4741 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     4801 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     4861 catgacccgc aagcccggtg cctgatctta tcgataatca acctctggat tacaaaattt
     4921 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4981 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     5041 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     5101 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     5161 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     5221 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     5281 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     5341 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     5401 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     5461 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc aaggcagctg tagatcttag
     5521 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     5581 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     5641 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5701 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     5761 agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta
     5821 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat
     5881 atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg
     5941 taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc
     6001 ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac
     6061 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
     6121 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
     6181 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
     6241 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
     6301 atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctagctctg
     6361 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     6421 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     6481 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     6541 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     6601 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6661 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6721 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6781 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6841 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6901 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6961 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     7021 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     7081 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     7141 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7201 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7261 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     7321 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     7381 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     7441 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7501 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7561 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7621 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7681 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7741 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7801 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7861 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     7921 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7981 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8041 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8101 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8161 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8221 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8281 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8341 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8401 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     8461 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     8521 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa
     8581 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     8641 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     8701 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     8761 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     8821 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     8881 aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga
     8941 tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg
     9001 actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt
     9061 tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa
     9121 aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga
     9181 tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc
     9241 gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga
     9301 tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt
     9361 catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta
     9421 tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga
     9481 ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt
     9541 attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt
     9601 tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca
     9661 tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct
     9721 cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg
     9781 gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca
     9841 ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta
     9901 ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt
     9961 gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta
    10021 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa
    10081 ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag
    10141 cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt
    10201 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta
    10261 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
    10321 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac
    10381 aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat
    10441 aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct
    10501 caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg
    10561 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc
    10621 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
    10681 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    10741 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    10801 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
    10861 caccccatac cctattacca ctgccaatta cctagtggtt tcatttactc taaacctgtg
    10921 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
    10981 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
    11041 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
    11101 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
    11161 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
    11221 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
    11281 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
    11341 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
    11401 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
    11461 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    11521 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    11581 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
    11641 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
    11701 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
    11761 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
    11821 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
    11881 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
    11941 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
    12001 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
    12061 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
    12121 gcacagcaag cggccggtga tcttcagacc tggacgatat atatgaggga caattggaga
    12181 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
    12241 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
    12301 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
    12361 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
    12421 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
    12481 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
    12541 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
    12601 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
    12661 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
    12721 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
    12781 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
    12841 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
    12901 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
    12961 gacagagaca gatccattcg attagtgaac ggatctcgac ggtcg
//
