LOCUS       V033259                12924 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033259
VERSION     V033259
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2380
                     /label="KLHL25(NM_022480)"
                     /note="KLHL25(NM_022480)"
                     /gene="KLHL25"
     CDS             2381..2407
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2453..2794
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2920..3419
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3440..4147
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4148..4204
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4205..4804
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4815..5403
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5466..5699
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5724..5742)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6531..6635
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6636..7496
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7667..8255
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8501..8830
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9964..10029
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10159..10179
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10604..10738
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10907..11540
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11587..11712
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12204..12437
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12622..12666
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttcttatcgt gaggcagtga
      601 actccaagcc accatgtcgg tcagtgtcca tgagacccgc aagtcgcgga gcagcacggg
      661 gtccatgaac gtcaccctct tccacaaggc ctcccacccg gactgtgtgc tggcccacct
      721 caacacgctt cgcaagcact gcatgttcac cgacgtcaca ctctgggcgg gcgaccgtgc
      781 cttcccctgt caccgtgccg tgctggccgc ctctagccgc tattttgagg ccatgttcag
      841 ccatggcctt cgggagagcc gggatgacac tgtcaacttc caggacaacc tgcacccgga
      901 ggtgctggag ctgctgctgg actttgccta ctcctcacgc atcgccatca acgaggagaa
      961 cgctgagtca ctgctggagg caggcgacat gctgcagttc cacgatgtgc gggatgctgc
     1021 cgccgagttc ctggagaaga accttttccc ctccaactgc ctgggcatga tgctgctctc
     1081 ggacgcccac cagtgccgcc ggctgtatga gttctcctgg cgcatgtgcc tggtgcactt
     1141 tgagacggtg aggcagagcg aggacttcaa cagcctgtcc aaggacacac tgctggacct
     1201 catctcgagt gatgagctgg agaccgagga cgagcgggtg gtcttcgagg ccatcctcca
     1261 gtgggtgaag cacgacctgg agccacggaa ggtccacttg cccgagctcc tccgcagcgt
     1321 gcgtctggcc ttgctgccgt ccgactgcct gcaggaggcc gtctccagcg aggccctcct
     1381 catggcagac gagcgcacca agcttatcat ggatgaggcc ctgcgctgca agaccaggat
     1441 cctgcagaat gatggcgtgg tcaccagccc ctgtgcccgg ccacgcaagg cgggccacac
     1501 gctactcatc ctggggggcc agaccttcat gtgtgacaag atctaccagg tggaccacaa
     1561 ggccaaggag atcatcccca aggccgacct gcccagcccc cggaaggagt tcagcgcctc
     1621 agcgatcggc tgcaaggtct atgtgacggg gggcaggggc tccgagaacg gggtctccaa
     1681 ggatgtctgg gtgtacgaca ccgtacatga ggaatggtcc aaggcggcgc ccatgctgat
     1741 tgcccgcttt ggccatggct cagctgagct ggagaactgc ctctatgtgg tggggggaca
     1801 cacatccctg gcaggggtct tcccggcctc gccttctgtc tccctgaaac aagtggagaa
     1861 atacgaccct ggggccaaca agtggatgat ggtggccccc ttgcgggatg gcgtcagcaa
     1921 tgccgcagtg gtgagtgcca agctgaagct ctttgttttc ggaggaacca gcatccaccg
     1981 ggacatggtg tccaaggtcc agtgctatga cccctcggag aacaggtgga cgatcaaggc
     2041 cgagtgcccc cagccttggc ggtacacagc cgctgccgtc ctgggcagcc agatcttcat
     2101 catgggaggt gacacggaat tcacagccgc ctcggcctac cgctttgact gtgagaccaa
     2161 ccagtggacg cggattgggg acatgactgc caagcgcatg tcctgccatg ccctggcttc
     2221 cggcaacaag ctctatgtgg tcgggggcta ctttgggacc cagaggtgta agactctgga
     2281 ctgctatgac cccacttcag atacatggaa ctgcatcacc acagtgccct actcacttat
     2341 ccccacggcc tttgtcagca cctggaagca cctgcccgcg tacccctacg acgtgcctga
     2401 ctacgccggc ggcggtggtt caggcggtgg cggttccggt ggtgggggat cagtccaact
     2461 ggtggagtct ggtggcgctt tggtgcagcc aggtggctct ctgcgtttgt cctgtgccgc
     2521 ttctggcttc ccagtgaacc gctattccat gcgctggtat cgccaggctc caggcaaaga
     2581 gcgtgagtgg gtagccggta tgtccagcgc gggtgatcgt agctcctatg aagactccgt
     2641 gaagggccgt ttcaccatca gccgtgacga tgcccgtaac acggtgtatc tgcaaatgaa
     2701 cagcttgaaa cctgaagata cggccgtgta ttactgtaat gtgaacgtgg gcttcgagta
     2761 ttggggccaa ggcacccagg tcaccgtctc cagctaagat ccttgacttg cggccgcaac
     2821 tcccacctgc aacatgcgtg actgactgag gccgcgactc tagagtcgac ctgcatctag
     2881 gcgccggaat tagatctctc gaggttaacg aattctaccg ggtaggggag gcgcttttcc
     2941 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
     3001 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
     3061 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     3121 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
     3181 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     3241 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     3301 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     3361 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     3421 cctgcagccc aagcttacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     3481 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     3541 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     3601 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     3661 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     3721 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     3781 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     3841 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     3901 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     3961 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     4021 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     4081 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     4141 gtacaaggca acaaacttct ctctgctgaa acaagccgga gatgtcgaag agaatcctgg
     4201 accgatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     4261 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     4321 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     4381 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     4441 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     4501 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     4561 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     4621 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     4681 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     4741 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     4801 ctgatcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4861 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4921 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     4981 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     5041 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     5101 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     5161 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     5221 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     5281 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     5341 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     5401 cgcatcgata ccgtcgacca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     5461 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     5521 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5581 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5641 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5701 catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat
     5761 aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta
     5821 gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt
     5881 ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc
     5941 gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc
     6001 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     6061 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     6121 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
     6181 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     6241 gagcgtcgaa tggtgcactc tcagtacaat ctagctctga tgccgcatag ttaagccagc
     6301 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     6361 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     6421 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     6481 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6541 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6601 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6661 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6721 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6781 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6841 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6901 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6961 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     7021 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7081 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7141 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     7201 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7261 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7321 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7381 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7441 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7501 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7561 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7621 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7681 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7741 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7801 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7861 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7921 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7981 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8041 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8101 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8161 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8221 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8281 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8341 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8401 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     8461 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg
     8521 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8581 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8641 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     8701 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8761 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8821 cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt
     8881 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
     8941 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
     9001 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
     9061 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
     9121 gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg
     9181 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
     9241 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
     9301 ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
     9361 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
     9421 agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
     9481 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt
     9541 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
     9601 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
     9661 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt
     9721 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg
     9781 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata
     9841 cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag
     9901 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
     9961 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
    10021 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
    10081 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
    10141 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
    10201 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
    10261 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
    10321 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
    10381 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
    10441 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
    10501 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
    10561 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
    10621 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    10681 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    10741 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    10801 tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
    10861 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
    10921 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
    10981 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
    11041 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
    11101 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
    11161 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
    11221 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
    11281 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
    11341 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
    11401 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    11461 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    11521 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
    11581 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
    11641 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
    11701 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
    11761 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
    11821 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
    11881 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
    11941 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
    12001 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggtgat
    12061 cttcagacct ggacgatata tatgagggac aattggagaa gtgaattata taaatataaa
    12121 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
    12181 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
    12241 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
    12301 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
    12361 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
    12421 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
    12481 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
    12541 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
    12601 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
    12661 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
    12721 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
    12781 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
    12841 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
    12901 ttagtgaacg gatctcgacg gtcg
//
