LOCUS       V033260                12984 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033260
VERSION     V033260
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2440
                     /label="KLHL20(NM_014458)"
                     /note="KLHL20(NM_014458)"
                     /gene="KLHL20"
     CDS             2441..2467
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2513..2854
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2980..3479
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3500..4207
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4208..4264
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4265..4864
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4875..5463
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5526..5759
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5784..5802)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6591..6695
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6696..7556
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7727..8315
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8561..8890
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10024..10089
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10219..10239
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10664..10798
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10967..11600
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11647..11772
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12264..12497
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12682..12726
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctgtgcac ttcgcagtga
      601 actccaagcc accatggaag gaaagccaat gcgcaggtgt accaacattc gaccaggaga
      661 gactggaatg gatgtaacaa gccgctgcac ccttggagac cccaacaaac tgccagaagg
      721 ggttccccaa cctgcccgca tgccctatat ctcagacaag caccctcgac aaaccttgga
      781 agtgattaac cttctgagaa agcaccggga gctatgtgat gtggtgctag ttgtgggcgc
      841 caagaagata tatgcccatc gagtcatttt gtcagcctgt agtccctact tccgagctat
      901 gtttacagga gaattggcag agagccgtca gacagaagta gtgatccgag acattgacga
      961 gagggctatg gaattactga ttgactttgc gtatacctcc cagataacag tagaagaggg
     1021 caatgttcag actcttctgc cagctgcttg cctcctccag ctggcagaaa tacaggaagc
     1081 ctgctgtgaa ttcttaaaga gacaattaga tccttctaac tgcctgggca ttcgggcttt
     1141 tgctgacaca cattcatgtc gtgagttgct aaggatagca gacaagttca cccaacataa
     1201 ctttcaagag gtaatggaga gtgaagagtt catgttgctt ccagccaatc aactcattga
     1261 tataatatcc agtgatgagc taaacgttcg cagtgaagaa caagtgttca atgcagtgat
     1321 ggcctgggtc aaatacagta ttcaggaaag acgtcctcaa ttaccccagg tgctgcagca
     1381 tgttcgtttg cctttgctta gtcccaagtt cctggtcggc acagtaggct ctgatcccct
     1441 catcaaaagt gatgaagaat gcagagactt ggtagatgag gctaaaaact acctcctatt
     1501 gccgcaagaa cgaccactaa tgcaaggacc aaggacgaga ccacggaaac ctatccgatg
     1561 tggggaagta ctctttgcag ttggtggttg gtgcagtgga gatgccattt ccagtgttga
     1621 acgatatgat ccacagacca atgaatggag aatggtggct tcaatgagca aaaggagatg
     1681 cggagttggg gtcagtgttc ttgatgatct gttatatgca gtaggaggcc atgatggatc
     1741 ctcttatctc aatagtgttg aaaggtatga ccccaaaaca aaccagtgga gcagtgatgt
     1801 ggcccctaca agcacctgca ggacaagtgt tggtgtagca gtacttggag gctttcttta
     1861 tgctgtgggt ggccaggatg gtgtgtcttg cctcaacatt gttgagaggt atgatccgaa
     1921 ggagaacaag tggactcggg tagcttctat gagtaccaga agactaggtg tggctgtggc
     1981 tgtgttagga gggttcttat atgctgtagg tggctctgac gggacatctc ctctcaacac
     2041 agtggaacgt tacaatcctc aggaaaacag atggcacact atagccccta tggggacccg
     2101 gaggaaacac ctaggctgtg cagtatatca ggacatgatc tatgctgtag gaggtagaga
     2161 tgacactaca gagctgagca gtgctgagag atacaacccc agaaccaacc agtggtctcc
     2221 agtggtggcc atgacatcac gccgtagtgg agttggcctg gcagtggtca atggacagct
     2281 catggcagta ggaggttttg atggcacaac atacttgaag accatagaag tttttgatcc
     2341 tgatgccaat acatggaggt tatatggcgg gatgaattac cgtcggctag ggggtggcgt
     2401 aggagttatt aaaatgacac attgtgaatc ccatatttgg tacccctacg acgtgcctga
     2461 ctacgccggc ggcggtggtt caggcggtgg cggttccggt ggtgggggat cagtccaact
     2521 ggtggagtct ggtggcgctt tggtgcagcc aggtggctct ctgcgtttgt cctgtgccgc
     2581 ttctggcttc ccagtgaacc gctattccat gcgctggtat cgccaggctc caggcaaaga
     2641 gcgtgagtgg gtagccggta tgtccagcgc gggtgatcgt agctcctatg aagactccgt
     2701 gaagggccgt ttcaccatca gccgtgacga tgcccgtaac acggtgtatc tgcaaatgaa
     2761 cagcttgaaa cctgaagata cggccgtgta ttactgtaat gtgaacgtgg gcttcgagta
     2821 ttggggccaa ggcacccagg tcaccgtctc cagctaagat ccttgacttg cggccgcaac
     2881 tcccacctgc aacatgcgtg actgactgag gccgcgactc tagagtcgac ctgcatctag
     2941 gcgccggaat tagatctctc gaggttaacg aattctaccg ggtaggggag gcgcttttcc
     3001 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
     3061 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
     3121 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     3181 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
     3241 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     3301 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     3361 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     3421 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     3481 cctgcagccc aagcttacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     3541 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     3601 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     3661 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     3721 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     3781 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     3841 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     3901 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     3961 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     4021 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     4081 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     4141 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     4201 gtacaaggca acaaacttct ctctgctgaa acaagccgga gatgtcgaag agaatcctgg
     4261 accgatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     4321 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     4381 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     4441 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     4501 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     4561 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     4621 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     4681 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     4741 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     4801 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     4861 ctgatcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4921 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4981 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     5041 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     5101 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     5161 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     5221 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     5281 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     5341 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     5401 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     5461 cgcatcgata ccgtcgacca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     5521 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     5581 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5641 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5701 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5761 catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat
     5821 aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta
     5881 gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt
     5941 ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc
     6001 gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc
     6061 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     6121 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     6181 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
     6241 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     6301 gagcgtcgaa tggtgcactc tcagtacaat ctagctctga tgccgcatag ttaagccagc
     6361 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     6421 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     6481 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     6541 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6601 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6661 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6721 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6781 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6841 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6901 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6961 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     7021 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     7081 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7141 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7201 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     7261 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7321 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7381 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7441 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7501 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7561 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7621 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7681 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7741 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7801 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7861 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7921 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7981 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8041 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8101 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8161 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8221 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8281 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8341 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8401 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8461 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     8521 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg
     8581 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8641 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8701 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     8761 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8821 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8881 cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt
     8941 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
     9001 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
     9061 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
     9121 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
     9181 gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg
     9241 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
     9301 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
     9361 ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
     9421 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
     9481 agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
     9541 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt
     9601 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
     9661 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
     9721 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt
     9781 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg
     9841 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata
     9901 cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag
     9961 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
    10021 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
    10081 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
    10141 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
    10201 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
    10261 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
    10321 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
    10381 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
    10441 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
    10501 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
    10561 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
    10621 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
    10681 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    10741 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    10801 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    10861 tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
    10921 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
    10981 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
    11041 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
    11101 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
    11161 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
    11221 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
    11281 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
    11341 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
    11401 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
    11461 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    11521 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    11581 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
    11641 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
    11701 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
    11761 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
    11821 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
    11881 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
    11941 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
    12001 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
    12061 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggtgat
    12121 cttcagacct ggacgatata tatgagggac aattggagaa gtgaattata taaatataaa
    12181 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
    12241 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
    12301 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
    12361 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
    12421 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
    12481 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
    12541 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
    12601 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
    12661 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
    12721 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
    12781 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
    12841 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
    12901 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
    12961 ttagtgaacg gatctcgacg gtcg
//
