LOCUS       V030107                14643 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030107
VERSION     V030107
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..4099
                     /label="KDM2A(NM_012308)"
                     /note="KDM2A(NM_012308)"
                     /gene="KDM2A"
     CDS             4100..4126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4172..4513
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        4639..5138
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5159..5866
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5867..5923
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             5924..6523
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6534..7122
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7185..7418
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(7443..7461)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        8250..8354
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             8355..9215
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      9386..9974
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        10220..10549
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          11683..11748
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             11878..11898
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    12323..12457
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             12626..13259
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    13306..13431
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    13923..14156
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             14341..14385
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctgtatat ccagcagtga
      601 actccaagcc accatggaac ccgaagaaga aaggattcgt tacagccaga gattgcgtgg
      661 taccatgcga cgacgctatg aagatgatgg catttcagat gatgaaattg aaggaaaaag
      721 aacttttgac ttggaagaga aactgcacac caacaaatat aatgccaatt ttgttacttt
      781 tatggaagga aaagatttta atgtagagta tattcagcgg ggtggcttga gagatcctct
      841 gattttcaag aattctgatg gactcggaat aaaaatgccg gatccagact tcactgtgaa
      901 tgatgtcaaa atgtgtgtgg ggagtcgtcg catggtggat gtcatggacg tgaacacaca
      961 gaaaggcatt gaaatgacca tggctcagtg gacacgctac tatgagaccc cagaggagga
     1021 gcgagagaaa ctctataatg tcatcagcct cgagtttagc cacaccaggc tggagaatat
     1081 ggtgcagagg ccctccacgg tggatttcat tgactgggta gacaacatgt ggccaaggca
     1141 cttgaaggaa agccagactg aatcaacaaa tgccatcttg gagatgcagt accctaaagt
     1201 gcagaagtac tgtctaatga gtgttcgagg ctgctatact gacttccatg tggactttgg
     1261 tggtacctct gtttggtatc acatccatca agggggaaag gtcttctggc tcatcccccc
     1321 tacagcccac aacctggagc tgtacgagaa ttggctgctg tcagggaaac agggagacat
     1381 ctttctgggt gaccgggtat cagattgtca gcgcattgag ctcaagcagg gctatacctt
     1441 cgtcattccc tcaggctgga ttcatgctgt gtatactcct acagacacat tagtgtttgg
     1501 gggcaatttt ttgcatagct tcaacatccc tatgcagtta aaaatataca acattgaaga
     1561 tcggacacgg gttccaaata agtttcgcta tccattctac tatgagatgt gttggtatgt
     1621 gttggagcgc tatgtgtact gcataaccaa ccgttcccac ctaactaagg aatttcagaa
     1681 agagtccctc agcatggatt tggagttaaa tgggttggag tctgggaatg gggatgagga
     1741 agcagtggat cgagaacccc gacgcttgag cagcaggcgt tctgtcctca ctagccctgt
     1801 agcgaatgga gtcaacctgg attatgatgg actgggcaaa acctgccgaa gtcttccaag
     1861 tctgaagaaa actttggctg gggactcatc ttctgactgt agccggggct cccacaatgg
     1921 acaagtgtgg gatccccagt gtgctccccg aaaggacagg caagtgcatc tgacccattt
     1981 tgagcttgaa ggccttcgct gccttgtaga taagttggag tctctgccac tgcacaagaa
     2041 atgtgtcccc acagggatag aagatgaaga tgctctcatt gctgatgtaa agattttgct
     2101 ggaggagctt gccaacagcg atcccaagtt agccctcact ggagttccta tagtacagtg
     2161 gccaaaaagg gataagctta aattccccac tcggccaaag gtgcgggttc ctaccatccc
     2221 cattacgaag cctcacacta tgaaaccagc tccacggtta acacctgtga ggccagctgc
     2281 tgcctccccg attgtgtcag gagccagacg gagacgagtg cgatgtcgaa aatgcaaagc
     2341 ctgtgtgcaa ggagagtgtg gtgtttgcca ctactgcaga gacatgaaga agtttggggg
     2401 gcctggacgc atgaagcagt cctgtgtcct ccgacagtgc ttggcaccca gactgcctca
     2461 ctcagtcaca tgttccctct gtggagaggt ggatcagaat gaagagacac aagactttga
     2521 gaagaaactc atggaatgct gtatctgcaa tgagattgtt catcctggct gcctccagat
     2581 ggacggagag gggttgctta acgaagaatt gccaaattgc tgggaatgtc caaagtgcta
     2641 ccaggaggac agctcggaga aagcccagaa gcggaaaatg gaagagagtg acgaagaagc
     2701 tgtgcaagcc aaagtcctgc ggcccctgcg gagctgcgat gagcctctca cgcccccgcc
     2761 tcattcaccc acttccatgc tgcagctcat ccatgacccg gtttcccccc ggggtatggt
     2821 gactcggtca tcccctgggg ctggccccag cgaccaccac agtgccagcc gcgatgagcg
     2881 cttcaaacgg cggcagttgc tgcggctgca ggccacagag cgcaccatgg tacgggaaaa
     2941 ggagaacaat cccagcggca aaaaggagct gtctgaagtt gagaaagcca agatccgggg
     3001 atcgtacctc actgtcacgc tacagaggcc caccaaagag ctccacggga catccattgt
     3061 gcccaagctg caggccatca cggcctcctc tgccaacctt cgccattccc cccgtgtgct
     3121 agtgcagcac tgcccagccc gaacccccca gcgtggggat gaggaggggc tggggggaga
     3181 ggaggaggaa gaggaggagg aggaggagga agatgacagt gcagaggagg ggggtgcagc
     3241 caggctgaat ggccggggca gttgggctca ggatggagac gaaagctgga tgcagcggga
     3301 ggtctggatg tctgtcttcc gctacctcag ccgcagagaa ctttgtgaat gtatgcgagt
     3361 gtgcaagacg tggtataaat ggtgctgcga caagagactt tggacaaaaa ttgacttgag
     3421 taggtgtaag gccattgtgc cccaggccct cagtggcatc atcaagaggc agccagtcag
     3481 ccttgacctc agttggacca acatctctaa aaagcaactg acatggctcg tcaataggct
     3541 gccaggactg aaagacctcc tcctagcagg ctgctcctgg tctgcagtct ctgccctcag
     3601 cacctccagc tgcccccttc tcaggaccct tgatcttcgg tgggcagtag gaatcaagga
     3661 ccctcaaatt cgggacttgc ttactccacc ggctgataaa ccaggtcagg acaatcgcag
     3721 caagctccgg aacatgaccg acttccggct ggcaggcctt gacatcacag atgccacgct
     3781 tcgcctcata attcgccaca tgcccctcct gtctcgactc gacctcagtc actgcagcca
     3841 ccttacagat cagtcctcca atctactcac tgctgtcggg tcttccactc gctactctct
     3901 cacagagctc aatatggcag gttgcaataa attgacagac cagaccctga tctacctacg
     3961 gcgcattgcc aacgtcacct tgatcgacct tcgaggatgc aagcagatca ctcgaaaagc
     4021 ctgcgagcac ttcatctcag acttgtccat caacagcctc tactgcctgt ctgacgagaa
     4081 gctgatacag aagatcagct acccctacga cgtgcctgac tacgccggcg gcggtggttc
     4141 aggcggtggc ggttccggtg gtgggggatc agtccaactg gtggagtctg gtggcgcttt
     4201 ggtgcagcca ggtggctctc tgcgtttgtc ctgtgccgct tctggcttcc cagtgaaccg
     4261 ctattccatg cgctggtatc gccaggctcc aggcaaagag cgtgagtggg tagccggtat
     4321 gtccagcgcg ggtgatcgta gctcctatga agactccgtg aagggccgtt tcaccatcag
     4381 ccgtgacgat gcccgtaaca cggtgtatct gcaaatgaac agcttgaaac ctgaagatac
     4441 ggccgtgtat tactgtaatg tgaacgtggg cttcgagtat tggggccaag gcacccaggt
     4501 caccgtctcc agctaagatc cttgacttgc ggccgcaact cccacctgca acatgcgtga
     4561 ctgactgagg ccgcgactct agagtcgacc tgcatctagg cgccggaatt agatctctcg
     4621 aggttaacga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     4681 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     4741 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     4801 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     4861 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     4921 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     4981 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     5041 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     5101 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     5161 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     5221 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     5281 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     5341 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     5401 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     5461 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     5521 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     5581 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     5641 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     5701 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     5761 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     5821 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaaggcaa caaacttctc
     5881 tctgctgaaa caagccggag atgtcgaaga gaatcctgga ccgatgaccg agtacaagcc
     5941 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     6001 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     6061 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     6121 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     6181 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     6241 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     6301 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     6361 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     6421 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     6481 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgatcttatc gataatcaac
     6541 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     6601 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     6661 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     6721 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     6781 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     6841 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6901 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     6961 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     7021 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     7081 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgaccaa
     7141 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     7201 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     7261 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7321 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7381 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt
     7441 attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag
     7501 ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag
     7561 accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga
     7621 gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc
     7681 gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     7741 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     7801 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     7861 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     7921 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct
     7981 cagtacaatc tagctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     8041 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     8101 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     8161 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     8221 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     8281 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     8341 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8401 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8461 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8521 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8581 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8641 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8701 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8761 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8821 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8881 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8941 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     9001 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     9061 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     9121 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     9181 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     9241 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9301 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9361 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9421 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9481 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     9541 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9601 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9661 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9721 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9781 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9841 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9901 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9961 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    10021 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    10081 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    10141 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    10201 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
    10261 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
    10321 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
    10381 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
    10441 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
    10501 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca
    10561 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
    10621 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
    10681 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
    10741 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta
    10801 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
    10861 gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt
    10921 ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga
    10981 aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt
    11041 tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg
    11101 tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc
    11161 gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct
    11221 ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta
    11281 ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct
    11341 ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg
    11401 tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga
    11461 tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga
    11521 tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg
    11581 gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg
    11641 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
    11701 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
    11761 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
    11821 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
    11881 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
    11941 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
    12001 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
    12061 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
    12121 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
    12181 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
    12241 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
    12301 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
    12361 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    12421 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    12481 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tagtggtttc
    12541 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    12601 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
    12661 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
    12721 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
    12781 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
    12841 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
    12901 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
    12961 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
    13021 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
    13081 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    13141 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    13201 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    13261 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
    13321 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
    13381 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
    13441 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
    13501 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
    13561 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
    13621 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
    13681 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
    13741 aacaaaagta agaccaccgc acagcaagcg gccggtgatc ttcagacctg gacgatatat
    13801 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
    13861 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
    13921 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
    13981 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
    14041 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
    14101 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
    14161 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
    14221 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
    14281 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    14341 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    14401 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    14461 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
    14521 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
    14581 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
    14641 tcg
//
