LOCUS       V033388                12144 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033388
VERSION     V033388
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..1600
                     /label="KCTD13(NM_178863)"
                     /note="KCTD13(NM_178863)"
                     /gene="KCTD13"
     CDS             1601..1627
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1673..2014
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2140..2639
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             2660..3367
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3368..3424
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3425..4024
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4035..4623
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             4686..4919
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(4944..4962)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        5751..5855
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             5856..6716
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      6887..7475
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        7721..8050
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9184..9249
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9379..9399
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    9824..9958
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10127..10760
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    10807..10932
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11424..11657
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             11842..11886
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctgcacct cacgcagtga
      601 actccaagcc accatgtcgg cggaggcctc gggcccggct gccgccgcgg ccccgtccct
      661 ggaagccccc aagccctcgg gtctcgagcc tggccccgcc gcctacggtc tcaagccgct
      721 gaccccgaac agcaaatacg tgaagctgaa cgtgggcggc tcgttgcact acaccacgct
      781 gcgcaccctc acgggacagg acaccatgct caaagccatg ttcagcggcc gcgtggaggt
      841 gctgaccgat gccggaggtt gggtgctgat tgaccggagc ggccgtcact ttggtacaat
      901 cctcaattac ctgcgggatg ggtctgtgcc actgccggag agtacgagag aactggggga
      961 gctgctgggc gaagcacgct actacctggt gcagggcctg attgaggact gccagctggc
     1021 gctgcagcaa aaaagggaga cgctgtcccc gctgtgcctc atccccatgg tgacatctcc
     1081 ccgggaggag cagcagctcc tggccagcac ctccaagccc gtggtgaagc tcctgcacaa
     1141 ccgcagtaac aacaagtact cctacaccag cacttcagat gacaacctac ttaagaacat
     1201 cgagctgttc gacaagctgg ccctgcgctt ccacgggcgg ctactcttcc tcaaggatgt
     1261 cctgggggac gagatctgct gctggtcttt ctacgggcag ggccgcaaaa tcgccgaggt
     1321 gtgctgcacc tccattgtct atgctacgga gaagaagcag accaaggtgg aatttccaga
     1381 ggcccggatc ttcgaggaga ccctgaacat cctcatctac gagactcccc ggggcccaga
     1441 cccagccctc ctggaggcca cagggggagc agctggagct ggtggggctg gccgcgggga
     1501 ggatgaagag aaccgagagc accgtgtccg caggatccat gtccggcgcc atatcaccca
     1561 cgacgagcgt cctcatggcc aacaaattgt cttcaaggac tacccctacg acgtgcctga
     1621 ctacgccggc ggcggtggtt caggcggtgg cggttccggt ggtgggggat cagtccaact
     1681 ggtggagtct ggtggcgctt tggtgcagcc aggtggctct ctgcgtttgt cctgtgccgc
     1741 ttctggcttc ccagtgaacc gctattccat gcgctggtat cgccaggctc caggcaaaga
     1801 gcgtgagtgg gtagccggta tgtccagcgc gggtgatcgt agctcctatg aagactccgt
     1861 gaagggccgt ttcaccatca gccgtgacga tgcccgtaac acggtgtatc tgcaaatgaa
     1921 cagcttgaaa cctgaagata cggccgtgta ttactgtaat gtgaacgtgg gcttcgagta
     1981 ttggggccaa ggcacccagg tcaccgtctc cagctaagat ccttgacttg cggccgcaac
     2041 tcccacctgc aacatgcgtg actgactgag gccgcgactc tagagtcgac ctgcatctag
     2101 gcgccggaat tagatctctc gaggttaacg aattctaccg ggtaggggag gcgcttttcc
     2161 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
     2221 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
     2281 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     2341 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
     2401 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     2461 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     2521 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     2581 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     2641 cctgcagccc aagcttacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     2701 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     2761 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     2821 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     2881 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     2941 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     3001 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     3061 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     3121 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     3181 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     3241 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     3301 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     3361 gtacaaggca acaaacttct ctctgctgaa acaagccgga gatgtcgaag agaatcctgg
     3421 accgatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     3481 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     3541 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     3601 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     3661 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     3721 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     3781 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     3841 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     3901 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     3961 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     4021 ctgatcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4081 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4141 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     4201 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     4261 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     4321 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4381 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     4441 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     4501 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     4561 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     4621 cgcatcgata ccgtcgacca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     4681 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     4741 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4801 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4861 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4921 catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat
     4981 aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta
     5041 gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt
     5101 ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc
     5161 gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc
     5221 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     5281 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     5341 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
     5401 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     5461 gagcgtcgaa tggtgcactc tcagtacaat ctagctctga tgccgcatag ttaagccagc
     5521 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5581 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5641 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     5701 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5761 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5821 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5881 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5941 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6001 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6061 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6121 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6181 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6241 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6301 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6361 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6421 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6481 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6541 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6601 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6661 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6721 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6781 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6841 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6901 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6961 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7021 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7081 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7141 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7201 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7261 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7321 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7381 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7441 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7501 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7561 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     7621 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     7681 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg
     7741 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7801 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7861 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     7921 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     7981 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8041 cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt
     8101 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
     8161 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
     8221 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
     8281 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
     8341 gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg
     8401 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
     8461 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
     8521 ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
     8581 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
     8641 agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
     8701 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt
     8761 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
     8821 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
     8881 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt
     8941 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg
     9001 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata
     9061 cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag
     9121 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
     9181 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
     9241 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
     9301 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
     9361 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
     9421 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
     9481 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
     9541 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
     9601 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
     9661 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
     9721 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     9781 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     9841 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     9901 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
     9961 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    10021 tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
    10081 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
    10141 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
    10201 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
    10261 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
    10321 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
    10381 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
    10441 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
    10501 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
    10561 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
    10621 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    10681 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    10741 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
    10801 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
    10861 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
    10921 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
    10981 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
    11041 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
    11101 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
    11161 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
    11221 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggtgat
    11281 cttcagacct ggacgatata tatgagggac aattggagaa gtgaattata taaatataaa
    11341 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
    11401 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
    11461 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
    11521 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
    11581 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
    11641 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
    11701 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
    11761 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
    11821 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
    11881 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
    11941 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
    12001 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
    12061 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
    12121 ttagtgaacg gatctcgacg gtcg
//
