LOCUS       V033602                13062 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033602
VERSION     V033602
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2518
                     /label="KCNC4(NM_004978)"
                     /note="KCNC4(NM_004978)"
                     /gene="KCNC4"
     CDS             2519..2545
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2591..2932
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3058..3557
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3578..4285
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4286..4342
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4343..4942
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4953..5541
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5604..5837
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5862..5880)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6669..6773
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6774..7634
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7805..8393
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8639..8968
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10102..10167
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10297..10317
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10742..10876
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11045..11678
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11725..11850
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12342..12575
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12760..12804
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttcttttgca cgggcagtga
      601 actccaagcc accatgatca gctcggtgtg tgtctcctcc taccgcgggc gcaagtcggg
      661 gaacaagcct ccgtccaaaa catgtctgaa ggaggagatg gccaagggcg aggcgtcgga
      721 gaagatcatc atcaacgtgg gcggcacgcg acatgagacc taccgcagca ccctgcgcac
      781 cctaccggga acccgcctcg cctggctggc cgaccccgac ggcgggggcc ggcccgagac
      841 cgatggcggc ggtgtgggta gcagcggcag cagcggcggc gggggctgcg agttcttctt
      901 cgacaggcac ccgggcgtct tcgcctacgt gctcaactac taccgcaccg gcaagctgca
      961 ctgccccgcg gacgtgtgcg ggccgctctt cgaagaggag ctcaccttct ggggcatcga
     1021 cgagaccgac gtggaaccct gctgctggat gacctaccgg cagcaccgcg acgccgagga
     1081 ggcgctcgac atcttcgaga gcccggacgg aggcggcagc ggcgcggggc ccagcgacga
     1141 ggccggcgac gatgagcggg agctggccct gcagcgactg ggcccccacg agggaggcgc
     1201 gggccatggc gccgggtctg ggggctgccg cggctggcag ccccgcatgt gggcgctctt
     1261 cgaggatccc tactcctccc gggccgctag ggtagtggcc tttgcctctc tcttcttcat
     1321 cctggtctcc atcaccactt tctgcctgga gacccatgag gcctttaata tcgaccgcaa
     1381 cgtgacagag atcctccgcg tagggaacat caccagcgtg cacttccggc gggaggtaga
     1441 gacagagccc atcctgacct acatcgaggg cgtatgtgtg ctgtggttca cactggagtt
     1501 cctggtgcgc atcgtgtgct gccccgacac gctggacttc gtcaagaacc tgctcaacat
     1561 catcgacttt gtggccatcc tgcccttcta cctggaggtg gggctgagcg gcctgtcatc
     1621 caaggcggcc cgcgacgtgc tgggcttcct gcgcgtggtg cgcttcgtgc gcatcctgcg
     1681 tatcttcaag ctcacacgcc acttcgtggg gctacgcgtg ctgggccaca ccctgagggc
     1741 cagcaccaat gagttcctgc tgcttatcat cttcctggcc ctgggtgtgc tcatctttgc
     1801 caccatgatc tactacgctg agcgcattgg ggccaggccc tccgaccctc ggggtaatga
     1861 ccacaccgac ttcaagaaca tccccattgg cttctggtgg gctgtggtca ccatgacgac
     1921 actgggctac ggagacatgt accccaagac gtggtcaggc atgctggtag gggcactgtg
     1981 tgcactggct ggcgtgctca ccatcgccat gccggtgcct gtcatcgtca acaacttcgg
     2041 catgtactac tccctggcca tggccaagca gaagctgccc aagaaacgga agaagcacgt
     2101 gccacggccg gcgcagctgg agtcacccat gtactgcaag tctgaggaga cttccccccg
     2161 ggacagcacc tgcagtgata ccagcccccc tgcccgggaa gagggtatga tcgagaggaa
     2221 acgggcagac tctaagcaga atggcgatgc caacgcagtg ctgtctgatg aggagggagc
     2281 tggcctcacc caacccctgg cctcctcccc gacccccgag gagcgccggg ccctgcgacg
     2341 ctccaccact cgagacagaa acaagaaggc agctgcctgc ttcctgctca gcactgggga
     2401 ctatgcctgc gccgatggta gtgtccggaa aggcacattc gtcctccgtg accttcccct
     2461 tcagcattca cctgaggctg catgccctcc aactgctggg actctgttcc tgccacatta
     2521 cccctacgac gtgcctgact acgccggcgg cggtggttca ggcggtggcg gttccggtgg
     2581 tgggggatca gtccaactgg tggagtctgg tggcgctttg gtgcagccag gtggctctct
     2641 gcgtttgtcc tgtgccgctt ctggcttccc agtgaaccgc tattccatgc gctggtatcg
     2701 ccaggctcca ggcaaagagc gtgagtgggt agccggtatg tccagcgcgg gtgatcgtag
     2761 ctcctatgaa gactccgtga agggccgttt caccatcagc cgtgacgatg cccgtaacac
     2821 ggtgtatctg caaatgaaca gcttgaaacc tgaagatacg gccgtgtatt actgtaatgt
     2881 gaacgtgggc ttcgagtatt ggggccaagg cacccaggtc accgtctcca gctaagatcc
     2941 ttgacttgcg gccgcaactc ccacctgcaa catgcgtgac tgactgaggc cgcgactcta
     3001 gagtcgacct gcatctaggc gccggaatta gatctctcga ggttaacgaa ttctaccggg
     3061 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     3121 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     3181 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     3241 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     3301 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     3361 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     3421 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     3481 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     3541 catctccggg cctttcgacc tgcagcccaa gcttaccatg gtgagcaagg gcgaggagga
     3601 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     3661 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     3721 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     3781 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     3841 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     3901 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     3961 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     4021 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     4081 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     4141 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     4201 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     4261 cggcggcatg gacgagctgt acaaggcaac aaacttctct ctgctgaaac aagccggaga
     4321 tgtcgaagag aatcctggac cgatgaccga gtacaagccc acggtgcgcc tcgccacccg
     4381 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     4441 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     4501 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     4561 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     4621 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     4681 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     4741 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     4801 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     4861 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     4921 gacccgcaag cccggtgcct gatcttatcg ataatcaacc tctggattac aaaatttgtg
     4981 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     5041 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     5101 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     5161 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     5221 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     5281 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     5341 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     5401 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     5461 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     5521 ccctttgggc cgcctccccg catcgatacc gtcgaccaag gcagctgtag atcttagcca
     5581 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     5641 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     5701 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     5761 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     5821 gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat
     5881 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
     5941 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
     6001 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
     6061 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
     6121 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     6181 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     6241 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     6301 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     6361 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct agctctgatg
     6421 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     6481 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     6541 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     6601 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     6661 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6721 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6781 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6841 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6901 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6961 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7021 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7081 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7141 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7201 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7261 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7321 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7381 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7441 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7501 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7561 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7621 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7681 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7741 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7801 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7861 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7921 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     7981 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8041 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8101 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8161 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8221 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8281 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8341 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8401 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8461 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8521 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8581 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
     8641 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     8701 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     8761 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     8821 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8881 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     8941 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
     9001 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     9061 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     9121 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     9181 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     9241 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
     9301 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     9361 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
     9421 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
     9481 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
     9541 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
     9601 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
     9661 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
     9721 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
     9781 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
     9841 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
     9901 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
     9961 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
    10021 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
    10081 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
    10141 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
    10201 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
    10261 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
    10321 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
    10381 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
    10441 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
    10501 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
    10561 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
    10621 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
    10681 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
    10741 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    10801 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    10861 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    10921 cccataccct attaccactg ccaattacct agtggtttca tttactctaa acctgtgatt
    10981 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
    11041 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
    11101 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
    11161 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
    11221 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
    11281 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
    11341 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
    11401 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
    11461 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
    11521 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
    11581 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
    11641 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
    11701 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
    11761 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
    11821 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
    11881 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
    11941 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
    12001 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
    12061 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
    12121 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
    12181 cagcaagcgg ccggtgatct tcagacctgg acgatatata tgagggacaa ttggagaagt
    12241 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
    12301 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
    12361 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
    12421 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
    12481 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
    12541 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
    12601 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
    12661 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
    12721 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
    12781 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
    12841 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
    12901 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
    12961 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
    13021 agagacagat ccattcgatt agtgaacgga tctcgacggt cg
//
