LOCUS       V033276                12525 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033276
VERSION     V033276
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..1981
                     /label="KCNA7(NM_031886)"
                     /note="KCNA7(NM_031886)"
                     /gene="KCNA7"
     CDS             1982..2008
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2054..2395
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2521..3020
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3041..3748
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3749..3805
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3806..4405
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4416..5004
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5067..5300
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5325..5343)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6132..6236
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6237..7097
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7268..7856
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8102..8431
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9565..9630
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9760..9780
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10205..10339
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10508..11141
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11188..11313
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11805..12038
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12223..12267
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctttggca gcggcagtga
      601 actccaagcc accatggagc cgcggtgccc gccgccgtgc ggctgctgcg agcggctggt
      661 gctcaacgtg gccgggctgc gcttcgagac gcgggcgcgc acgctgggcc gcttcccgga
      721 cactctgcta ggggacccag cgcgccgcgg ccgcttctac gacgacgcgc gccgcgagta
      781 tttcttcgac cggcaccggc ccagcttcga cgccgtgctc tactactacc agtccggtgg
      841 gcggctgcgg cggccggcgc acgtgccgct cgacgtcttc ctggaagagg tggccttcta
      901 cgggctgggc gcggcggccc tggcacgcct gcgcgaggac gagggctgcc cggtgccgcc
      961 cgagcgcccc ctgccccgcc gcgccttcgc ccgccagctg tggctgcttt tcgagtttcc
     1021 cgagagctct caggccgcgc gcgtgctcgc cgtagtctcc gtgctggtca tcctcgtctc
     1081 catcgtcgtc ttctgcctcg agacgctgcc tgacttccgc gacgaccgcg acggcacggg
     1141 gcttgctgct gcagccgcag ccggcccgtt ccccgctccg ctgaatggct ccagccaaat
     1201 gcctggaaat ccaccccgcc tgcccttcaa tgacccgttc ttcgtggtgg agacgctgtg
     1261 tatttgttgg ttctcctttg agctgctggt acgcctcctg gtctgtccaa gcaaggctat
     1321 cttcttcaag aacgtgatga acctcatcga ttttgtggct atccttccct actttgtggc
     1381 actgggcacc gagctggccc ggcagcgagg ggtgggccag caggccatgt cactggccat
     1441 cctgagagtc atccgattgg tgcgtgtctt ccgcatcttc aagctgtccc ggcactcaaa
     1501 gggcctgcaa atcttgggcc agacgcttcg ggcctccatg cgtgagctgg gcctcctcat
     1561 ctttttcctc ttcatcggtg tggtcctctt ttccagcgcc gtctactttg ccgaagttga
     1621 ccgggtggac tcccatttca ctagcatccc tgagtccttc tggtgggcgg tagtcaccat
     1681 gactacagtt ggctatggag acatggcacc cgtcactgtg ggtggcaaga tagtgggctc
     1741 tctgtgtgcc attgcgggcg tgctgactat ttccctgcca gtgcccgtca ttgtctccaa
     1801 tttcagctac ttttatcacc gggagacaga gggcgaagag gctgggatgt tcagccatgt
     1861 ggacatgcag ccttgtggcc cactggaggg caaggccaat ggggggctgg tggacgggga
     1921 ggtacctgag ctaccacctc cactctgggc acccccaggg aaacacctgg tcaccgaagt
     1981 gtacccctac gacgtgcctg actacgccgg cggcggtggt tcaggcggtg gcggttccgg
     2041 tggtggggga tcagtccaac tggtggagtc tggtggcgct ttggtgcagc caggtggctc
     2101 tctgcgtttg tcctgtgccg cttctggctt cccagtgaac cgctattcca tgcgctggta
     2161 tcgccaggct ccaggcaaag agcgtgagtg ggtagccggt atgtccagcg cgggtgatcg
     2221 tagctcctat gaagactccg tgaagggccg tttcaccatc agccgtgacg atgcccgtaa
     2281 cacggtgtat ctgcaaatga acagcttgaa acctgaagat acggccgtgt attactgtaa
     2341 tgtgaacgtg ggcttcgagt attggggcca aggcacccag gtcaccgtct ccagctaaga
     2401 tccttgactt gcggccgcaa ctcccacctg caacatgcgt gactgactga ggccgcgact
     2461 ctagagtcga cctgcatcta ggcgccggaa ttagatctct cgaggttaac gaattctacc
     2521 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     2581 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     2641 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     2701 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     2761 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     2821 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     2881 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     2941 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     3001 cctcatctcc gggcctttcg acctgcagcc caagcttacc atggtgagca agggcgagga
     3061 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     3121 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     3181 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     3241 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     3301 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     3361 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     3421 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     3481 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     3541 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     3601 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     3661 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     3721 caccggcggc atggacgagc tgtacaaggc aacaaacttc tctctgctga aacaagccgg
     3781 agatgtcgaa gagaatcctg gaccgatgac cgagtacaag cccacggtgc gcctcgccac
     3841 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     3901 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     3961 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     4021 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     4081 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     4141 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     4201 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     4261 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     4321 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     4381 catgacccgc aagcccggtg cctgatctta tcgataatca acctctggat tacaaaattt
     4441 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4501 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4561 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4621 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4681 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4741 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4801 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     4861 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4921 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4981 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc aaggcagctg tagatcttag
     5041 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     5101 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     5161 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5221 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     5281 agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta
     5341 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat
     5401 atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg
     5461 taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc
     5521 ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac
     5581 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
     5641 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
     5701 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
     5761 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
     5821 atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctagctctg
     5881 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     5941 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     6001 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     6061 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     6121 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6181 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6241 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6301 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6361 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6421 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6481 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6541 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6601 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6661 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6721 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6781 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6841 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6901 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6961 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7021 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7081 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7141 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7201 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7261 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7321 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7381 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     7441 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7501 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7561 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7621 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7681 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7741 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7801 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7861 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7921 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7981 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     8041 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa
     8101 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     8161 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     8221 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     8281 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     8341 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     8401 aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga
     8461 tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg
     8521 actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt
     8581 tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa
     8641 aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga
     8701 tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc
     8761 gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga
     8821 tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt
     8881 catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta
     8941 tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga
     9001 ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt
     9061 attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt
     9121 tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca
     9181 tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct
     9241 cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg
     9301 gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca
     9361 ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta
     9421 ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt
     9481 gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta
     9541 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa
     9601 ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag
     9661 cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt
     9721 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta
     9781 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
     9841 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac
     9901 aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat
     9961 aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct
    10021 caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg
    10081 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc
    10141 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
    10201 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    10261 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    10321 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
    10381 caccccatac cctattacca ctgccaatta cctagtggtt tcatttactc taaacctgtg
    10441 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
    10501 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
    10561 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
    10621 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
    10681 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
    10741 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
    10801 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
    10861 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
    10921 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
    10981 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    11041 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    11101 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
    11161 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
    11221 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
    11281 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
    11341 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
    11401 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
    11461 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
    11521 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
    11581 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
    11641 gcacagcaag cggccggtga tcttcagacc tggacgatat atatgaggga caattggaga
    11701 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
    11761 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
    11821 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
    11881 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
    11941 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
    12001 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
    12061 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
    12121 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
    12181 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
    12241 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
    12301 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
    12361 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
    12421 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
    12481 gacagagaca gatccattcg attagtgaac ggatctcgac ggtcg
//
