LOCUS       V033767                12690 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033767
VERSION     V033767
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2146
                     /label="KCNA10(NM_005549)"
                     /note="KCNA10(NM_005549)"
                     /gene="KCNA10"
     CDS             2147..2173
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2219..2560
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2686..3185
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3206..3913
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3914..3970
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3971..4570
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4581..5169
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5232..5465
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5490..5508)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6297..6401
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6402..7262
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7433..8021
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8267..8596
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9730..9795
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9925..9945
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10370..10504
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10673..11306
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11353..11478
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11970..12203
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12388..12432
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctccatta ggagcagtga
      601 actccaagcc accatggatg tgtgtggctg gaaagaaatg gaggttgcgc tggtcaattt
      661 tgataattca gatgaaatcc aagaagagcc aggctatgcc acagacttcg actcaaccag
      721 cccaaaaggc cggcctgggg gcagctcctt ctccaacggg aagatcctca tcagcgaaag
      781 caccaaccat gagacggcct tctccaagct tccgggagac tatgctgacc ccccagggcc
      841 tgagccagtg gtcctaaatg aaggaaacca gcgggtgatc atcaacattg ctgggctgag
      901 atttgagacc cagctcagaa cccttagtca gttcccagag actctcctgg gagaccggga
      961 aaaaaggatg cagttctttg actccatgag aaatgagtat ttctttgatc ggaaccggcc
     1021 cagttttgat ggaatcctat attattacca atctggtggg aaaattcggc gcccagccaa
     1081 tgttcccatt gatatctttg ctgatgaaat ctccttctat gagctgggta gtgaggccat
     1141 ggaccagttc cgggaggatg aaggcttcat caaagaccct gaaacactgc tacccaccaa
     1201 tgacatccac cgtcagttct ggctcctctt tgagtaccct gaaagttcca gcgctgcccg
     1261 tgctgtggcc gtggtctcgg tgttggttgt ggtcatctcc atcaccatct tctgcctgga
     1321 gacactgcca gagttccggg aggataggga gctaaaggtg gtcagagacc ccaatctcaa
     1381 catgagcaag acagtcctct cccagaccat gttcaccgac cctttcttca tggtggagtc
     1441 tacctgcatc gtgtggttca ccttcgagct ggtgctccgg ttcgtggtct gccccagcaa
     1501 gactgacttc ttcaggaaca tcatgaacat cattgacatc atctccatta tcccctactt
     1561 tgcaactctc atcacagagc tagtccagga gacagagccg agtgcccaac agaacatgtc
     1621 cctggccatc ctgaggatca tccgcctggt gagggtcttc cgcatcttca agctctcgcg
     1681 ccactccaag gggctgcaga tcctcgggca aacactgaag gcgtccatgc gggagttggg
     1741 gttgctcatc ttctttctct tcattggagt catcctcttc tccagtgcag tctactttgc
     1801 tgaggtggat gagccagagt cccatttctc tagcattcct gatggcttct ggtgggcagt
     1861 ggtcaccatg acaactgtag gctatgggga catgtgcccg accaccccag gggggaagat
     1921 tgtgggcact ctgtgtgcca ttgcaggggt cctcaccatt gccctccctg tgcctgtcat
     1981 tgtctccaac ttcaattact tctaccaccg ggagactgag aatgaagaaa agcagaacat
     2041 cccaggagaa attgaaagaa tcctcaacag tgtaggctca agaatgggca gcacagactc
     2101 tcttaataag accaatggtg gctgttccac agagaagtct aggaaatacc cctacgacgt
     2161 gcctgactac gccggcggcg gtggttcagg cggtggcggt tccggtggtg ggggatcagt
     2221 ccaactggtg gagtctggtg gcgctttggt gcagccaggt ggctctctgc gtttgtcctg
     2281 tgccgcttct ggcttcccag tgaaccgcta ttccatgcgc tggtatcgcc aggctccagg
     2341 caaagagcgt gagtgggtag ccggtatgtc cagcgcgggt gatcgtagct cctatgaaga
     2401 ctccgtgaag ggccgtttca ccatcagccg tgacgatgcc cgtaacacgg tgtatctgca
     2461 aatgaacagc ttgaaacctg aagatacggc cgtgtattac tgtaatgtga acgtgggctt
     2521 cgagtattgg ggccaaggca cccaggtcac cgtctccagc taagatcctt gacttgcggc
     2581 cgcaactccc acctgcaaca tgcgtgactg actgaggccg cgactctaga gtcgacctgc
     2641 atctaggcgc cggaattaga tctctcgagg ttaacgaatt ctaccgggta ggggaggcgc
     2701 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     2761 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     2821 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     2881 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     2941 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     3001 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     3061 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     3121 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     3181 tttcgacctg cagcccaagc ttaccatggt gagcaagggc gaggaggata acatggccat
     3241 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     3301 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     3361 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     3421 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     3481 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     3541 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     3601 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     3661 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     3721 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     3781 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     3841 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     3901 cgagctgtac aaggcaacaa acttctctct gctgaaacaa gccggagatg tcgaagagaa
     3961 tcctggaccg atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     4021 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     4081 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     4141 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     4201 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     4261 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     4321 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     4381 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     4441 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     4501 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     4561 cggtgcctga tcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     4621 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4681 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4741 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4801 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4861 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4921 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     4981 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5041 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5101 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5161 cctccccgca tcgataccgt cgaccaaggc agctgtagat cttagccact ttttaaaaga
     5221 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     5281 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     5341 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     5401 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     5461 tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg
     5521 atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat
     5581 tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga
     5641 gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca cccggaaatg
     5701 gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg
     5761 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     5821 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     5881 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     5941 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     6001 aagggagagc gtcgaatggt gcactctcag tacaatctag ctctgatgcc gcatagttaa
     6061 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     6121 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     6181 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     6241 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     6301 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6361 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6421 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6481 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6541 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6601 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     6661 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6721 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6781 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6841 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6901 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6961 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     7021 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7081 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7141 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7201 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7261 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7321 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7381 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7441 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7501 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7561 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7621 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7681 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7741 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7801 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7861 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7921 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7981 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8041 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8101 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8161 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     8221 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg
     8281 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8341 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     8401 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     8461 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8521 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     8581 cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac
     8641 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
     8701 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
     8761 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
     8821 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
     8881 ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga
     8941 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
     9001 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
     9061 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
     9121 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
     9181 cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
     9241 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
     9301 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
     9361 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
     9421 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
     9481 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa
     9541 tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat
     9601 acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt
     9661 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     9721 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     9781 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     9841 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     9901 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
     9961 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
    10021 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
    10081 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
    10141 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
    10201 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
    10261 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
    10321 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
    10381 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    10441 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    10501 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
    10561 taccactgcc aattacctag tggtttcatt tactctaaac ctgtgattcc tctgaattat
    10621 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
    10681 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
    10741 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
    10801 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
    10861 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
    10921 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
    10981 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
    11041 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
    11101 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
    11161 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    11221 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
    11281 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
    11341 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
    11401 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
    11461 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
    11521 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
    11581 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
    11641 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
    11701 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
    11761 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
    11821 ggtgatcttc agacctggac gatatatatg agggacaatt ggagaagtga attatataaa
    11881 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
    11941 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
    12001 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
    12061 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
    12121 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
    12181 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
    12241 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
    12301 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
    12361 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
    12421 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
    12481 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
    12541 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
    12601 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
    12661 attcgattag tgaacggatc tcgacggtcg
//
