LOCUS       V033764                13026 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033764
VERSION     V033764
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2482
                     /label="KBTBD12(NM_207335)"
                     /note="KBTBD12(NM_207335)"
                     /gene="KBTBD12"
     CDS             2483..2509
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2555..2896
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3022..3521
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3542..4249
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4250..4306
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4307..4906
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4917..5505
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5568..5801
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5826..5844)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6633..6737
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6738..7598
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7769..8357
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8603..8932
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10066..10131
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10261..10281
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10706..10840
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11009..11642
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11689..11814
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12306..12539
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12724..12768
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctagcccc attgcagtga
      601 actccaagcc accatggagt gcaagattga gggaaaagaa aaataccaac atagcttgaa
      661 tttactgaat aaaattcaga acatgaaaga attagcagaa atgattgatg tggtactcac
      721 agcagaagga gagaaatttc cttgccacag actggtcctg gctgcattta gcccttattt
      781 caaagctatg ttcacctgtg gactacttga atgtaatcaa agggaagtca tactttatga
      841 catcacagca gaaagtgtgt cggtgttatt aaattacatg tacaatgcag ctttggagat
      901 caataatgcc aatgtacaga ctgtagctat ggctgcctat tttatgcaga tggaagaagt
      961 cttcagtgtg tgtcaaaaat atatgatgga ccacatggat gcctccaact gtttaggtat
     1021 ctattatttt gcaaagcaga ttggagctga agatttatct gatcgatcaa agaaatattt
     1081 atatcagcac tttgccgagg tgagcttaca tgaagaaata ctagaaatcg aagtgcacca
     1141 atttttgaca cttattaaat cagatgatct taacatatcc agagaagaga gcattctgga
     1201 cttagttctg agatgggtaa atcataacaa agaattgcgt acagtgcatc ttgttgagct
     1261 tttgaagcaa gtcagattgg aacttgtaaa tccttctttt ttaagacaag ccctaagaag
     1321 gaacacaatg cttctgtgtg atgcagattg tgttgacata attcaaaatg cattcaaagc
     1381 catcaagaca ccccaacagc actctctaaa tctgcgctat ggtatggaga ctaccagtct
     1441 tctgctttgc attggcaaca attcttcagg aatcagatca agacatagga gctatgggga
     1501 tgccagtttt tgttatgatc ctgtatcacg gaaaacctat ttcatctcat ctcccaagta
     1561 cggagagggt ttaggaactg tgtgtactgg tgttgtcatg gaaaataata ctataattgt
     1621 ggctggagaa gcaagtgcct ctaaactctc tagacaaaag aacaagaatg ttgaaattta
     1681 taggtatcat gatagaggaa accagttttg ggaaaagtta tgcacagctg aatttcgaga
     1741 actctatgct ctgggcagta ttcataatga cctttatgtt ataggaggac agatgaaaat
     1801 taaaaaccag tatcttatta caaactgtgt tgataagtac tctgtagaac gggacaattg
     1861 gaaaagggtg tctccccttc cactgcaatt ggcatgtcat gctgtagtga cagtgaataa
     1921 taaactttat gtaattggag gctggacccc tcagatggat cttcctgatg aagaacctga
     1981 tcgattaagc aacaaactgt tgcagtatga ccccagccaa gatcaatgga gtgtgcgggc
     2041 acccatgaag tactctaagt accgattcag tacagctgta gtcaacagtg agatttatgt
     2101 tttgggtggc attggctgtg taggtcaaga caagggccag gttcgaaaat gccttgacgt
     2161 ggtggagatc tacaacccag atggggactt ttggcgagag ggccctccca tgccaagtcc
     2221 cctcctctca ctccgcacca attccaccaa tgcaggggca gtggatggga aactctatgt
     2281 ctgcggggga ttccatggag cagatcgcca tgaggttatc tccaaagaaa tattggaact
     2341 ggacccatgg gaaaaccagt ggaatgttgt agccatcaac gtcctcatgc atgacagcta
     2401 tgatgtctgc ctagtagcca ggatgaatcc ccgagacctc atccccccgc cttcagattt
     2461 ggtggaagaa ggcaatgagc actaccccta cgacgtgcct gactacgccg gcggcggtgg
     2521 ttcaggcggt ggcggttccg gtggtggggg atcagtccaa ctggtggagt ctggtggcgc
     2581 tttggtgcag ccaggtggct ctctgcgttt gtcctgtgcc gcttctggct tcccagtgaa
     2641 ccgctattcc atgcgctggt atcgccaggc tccaggcaaa gagcgtgagt gggtagccgg
     2701 tatgtccagc gcgggtgatc gtagctccta tgaagactcc gtgaagggcc gtttcaccat
     2761 cagccgtgac gatgcccgta acacggtgta tctgcaaatg aacagcttga aacctgaaga
     2821 tacggccgtg tattactgta atgtgaacgt gggcttcgag tattggggcc aaggcaccca
     2881 ggtcaccgtc tccagctaag atccttgact tgcggccgca actcccacct gcaacatgcg
     2941 tgactgactg aggccgcgac tctagagtcg acctgcatct aggcgccgga attagatctc
     3001 tcgaggttaa cgaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     3061 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     3121 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     3181 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     3241 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     3301 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     3361 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     3421 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     3481 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
     3541 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     3601 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     3661 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     3721 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     3781 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     3841 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     3901 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     3961 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     4021 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     4081 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     4141 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     4201 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg caacaaactt
     4261 ctctctgctg aaacaagccg gagatgtcga agagaatcct ggaccgatga ccgagtacaa
     4321 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     4381 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     4441 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     4501 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     4561 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     4621 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     4681 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     4741 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     4801 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     4861 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgatctt atcgataatc
     4921 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     4981 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5041 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5101 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5161 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     5221 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5281 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     5341 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     5401 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     5461 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     5521 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     5581 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     5641 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5701 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     5761 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
     5821 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
     5881 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
     5941 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
     6001 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
     6061 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     6121 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     6181 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     6241 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     6301 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
     6361 tctcagtaca atctagctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     6421 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     6481 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     6541 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     6601 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6661 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6721 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     6781 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6841 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     6901 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     6961 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7021 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7081 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7141 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7201 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7261 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7321 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7381 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7441 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7501 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7561 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     7621 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     7681 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7741 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     7801 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7861 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     7921 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7981 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8041 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8101 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8161 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8221 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8281 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8341 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8401 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8461 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     8521 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     8581 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     8641 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     8701 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     8761 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8821 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     8881 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
     8941 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
     9001 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
     9061 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
     9121 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
     9181 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
     9241 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     9301 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
     9361 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
     9421 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
     9481 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
     9541 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
     9601 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa
     9661 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
     9721 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
     9781 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
     9841 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg
     9901 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg
     9961 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt
    10021 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
    10081 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
    10141 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
    10201 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
    10261 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
    10321 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
    10381 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
    10441 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
    10501 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
    10561 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
    10621 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
    10681 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
    10741 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    10801 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    10861 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctagtggt
    10921 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    10981 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
    11041 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
    11101 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
    11161 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
    11221 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
    11281 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
    11341 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
    11401 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
    11461 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    11521 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    11581 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    11641 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
    11701 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
    11761 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
    11821 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
    11881 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
    11941 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
    12001 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
    12061 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    12121 caaaacaaaa gtaagaccac cgcacagcaa gcggccggtg atcttcagac ctggacgata
    12181 tatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
    12241 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
    12301 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
    12361 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
    12421 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
    12481 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
    12541 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
    12601 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
    12661 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
    12721 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
    12781 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
    12841 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
    12901 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
    12961 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
    13021 cggtcg
//
