LOCUS       V030080                13866 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030080
VERSION     V030080
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..3322
                     /label="ITCH(NM_001257137)"
                     /note="ITCH(NM_001257137)"
                     /gene="ITCH"
     CDS             3323..3349
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3395..3736
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3862..4361
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4382..5089
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5090..5146
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             5147..5746
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5757..6345
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6408..6641
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6666..6684)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7473..7577
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7578..8438
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8609..9197
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9443..9772
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10906..10971
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             11101..11121
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11546..11680
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11849..12482
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12529..12654
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    13146..13379
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13564..13608
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctgtaaca gttgcagtga
      601 actccaagcc accatgtctg acagtggatc acaacttggt tcaatgggta gcctcaccat
      661 gaaatcacag cttcagatca ctgtcatctc agcaaaactt aaggaaaata agaagaattg
      721 gtttggacca agtccttacg tagaggtcac agtagatgga cagtcaaaga agacagaaaa
      781 atgcaacaac acaaacagtc ccaagtggaa gcaacccctt acagttatcg ttacccctgt
      841 gagtaaatta cattttcgtg tgtggagtca ccagacactg aaatctgatg ttttgttggg
      901 aactgctgca ttagatattt atgaaacatt aaagtcaaac aatatgaaac ttgaagaagt
      961 agttgtgact ttgcagcttg gaggtgacaa agagccaaca gagacaatag gagacttgtc
     1021 aatttgtctt gatgggctac agttagagtc tgaagttgtt accaatggtg aaactacatg
     1081 ttcagaaaat ggggtttcgc tctgtttgcc caggctggag tgcaatagtg cgatctcggc
     1141 tcactgcaac ctctgcctcc cgggtttaag tgattctcct atctcagcct cccgagtagc
     1201 tgggtttaca ggtgcttctc agaatgatga tggctccaga tccaaggatg aaacaagagt
     1261 gagcacaaat ggatcagatg accctgaaga tgcaggagct ggtgaaaata ggagagtcag
     1321 tgggaataat tctccatcac tctcaaatgg tggttttaaa ccttctagac ctccaagacc
     1381 ttcacgacca ccaccaccca ccccacgtag accagcatct gtcaatggtt caccatctgc
     1441 cacttctgaa agtgatgggt ctagtacagg ctctctgccg ccgacaaata caaatacaaa
     1501 tacatctgaa ggagcaacat ctggattaat aattcctctt actatatctg gaggctcagg
     1561 ccctaggcca ttaaatcctg taactcaagc tcccttgcca cctggttggg agcagagagt
     1621 ggaccagcac gggcgagttt actatgtaga tcatgttgag aaaagaacaa catgggatag
     1681 accagaacct ctacctcctg gctgggaacg gcgggttgac aacatgggac gtatttatta
     1741 tgttgaccat ttcacaagaa caacaacgtg gcagaggcca acactggaat ccgtccggaa
     1801 ctatgaacaa tggcagctac agcgtagtca gcttcaagga gcaatgcagc agtttaacca
     1861 gagattcatt tatgggaatc aagatttatt tgctacatca caaagtaaag aatttgatcc
     1921 tcttggtcca ttgccacctg gatgggagaa gagaacagac agcaatggca gagtatattt
     1981 cgtcaaccac aacacacgaa ttacacaatg ggaagacccc agaagtcaag gtcaattaaa
     2041 tgaaaagccc ttacctgaag gttgggaaat gagattcaca gtggatggaa ttccatattt
     2101 tgtggaccac aatagaagaa ctaccaccta tatagatccc cgcacaggaa aatctgccct
     2161 agacaatgga cctcagatag cctatgttcg ggacttcaaa gcaaaggttc agtatttccg
     2221 gttctggtgt cagcaactgg ccatgccaca gcacataaag attacagtga caagaaaaac
     2281 attgtttgag gattcctttc aacagataat gagcttcagt ccccaagatc tgcgaagacg
     2341 tttgtgggtg atttttccag gagaagaagg tttagattat ggaggtgtag caagagaatg
     2401 gttctttctt ttgtcacatg aagtgttgaa cccaatgtat tgcctgtttg aatatgcagg
     2461 gaaggataac tactgcttgc agataaaccc cgcttcttac atcaatccag atcacctgaa
     2521 atattttcgt tttattggca gatttattgc catggctctg ttccatggga aattcataga
     2581 cacgggtttt tctttaccat tctataagcg tatcttgaac aaaccagttg gactcaagga
     2641 tttagaatct attgatccag aattttacaa ttctctcatc tgggttaagg aaaacaatat
     2701 tgaggaatgt gatttggaaa tgtacttctc cgttgacaaa gaaattctag gtgaaattaa
     2761 gagtcatgat ctgaaaccta atggtggcaa tattcttgta acagaagaaa ataaagagga
     2821 atacatcaga atggtagctg agtggaggtt gtctcgaggt gttgaagaac agacacaagc
     2881 tttctttgaa ggctttaatg aaattcttcc ccagcaatat ttgcaatact ttgatgcaaa
     2941 ggaattagag gtccttttat gtggaatgca agagattgat ttgaatgact ggcaaagaca
     3001 tgccatctac cgtcattatg caaggaccag caaacaaatc atgtggtttt ggcagtttgt
     3061 taaagaaatt gataatgaga agagaatgag acttctgcag tttgttactg gaacctgccg
     3121 attgccagta ggaggatttg ctgatctcat ggggagcaat ggaccacaga aattctgcat
     3181 tgaaaaagtt gggaaagaaa attggctacc cagaagtcat acctgtttta atcgcctgga
     3241 cctgccacca tacaagagct atgagcaact gaaggaaaag ctgttgtttg ccatagaaga
     3301 aacagaagga tttggacaag agtaccccta cgacgtgcct gactacgccg gcggcggtgg
     3361 ttcaggcggt ggcggttccg gtggtggggg atcagtccaa ctggtggagt ctggtggcgc
     3421 tttggtgcag ccaggtggct ctctgcgttt gtcctgtgcc gcttctggct tcccagtgaa
     3481 ccgctattcc atgcgctggt atcgccaggc tccaggcaaa gagcgtgagt gggtagccgg
     3541 tatgtccagc gcgggtgatc gtagctccta tgaagactcc gtgaagggcc gtttcaccat
     3601 cagccgtgac gatgcccgta acacggtgta tctgcaaatg aacagcttga aacctgaaga
     3661 tacggccgtg tattactgta atgtgaacgt gggcttcgag tattggggcc aaggcaccca
     3721 ggtcaccgtc tccagctaag atccttgact tgcggccgca actcccacct gcaacatgcg
     3781 tgactgactg aggccgcgac tctagagtcg acctgcatct aggcgccgga attagatctc
     3841 tcgaggttaa cgaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     3901 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     3961 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     4021 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     4081 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     4141 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     4201 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     4261 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     4321 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
     4381 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     4441 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     4501 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     4561 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     4621 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     4681 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     4741 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     4801 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     4861 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     4921 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     4981 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     5041 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg caacaaactt
     5101 ctctctgctg aaacaagccg gagatgtcga agagaatcct ggaccgatga ccgagtacaa
     5161 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     5221 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     5281 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     5341 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     5401 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     5461 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     5521 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     5581 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     5641 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     5701 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgatctt atcgataatc
     5761 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5821 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5881 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5941 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6001 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6061 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6121 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     6181 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6241 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     6301 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     6361 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     6421 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     6481 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6541 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6601 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
     6661 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
     6721 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
     6781 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
     6841 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
     6901 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     6961 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     7021 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     7081 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     7141 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
     7201 tctcagtaca atctagctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     7261 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     7321 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     7381 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     7441 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7501 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7561 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7621 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7681 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7741 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7801 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7861 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7921 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7981 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8041 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8101 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8161 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8221 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8281 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8341 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8401 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8461 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8521 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8581 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8641 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8701 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     8761 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8821 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8881 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8941 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9001 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9061 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9121 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9181 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9241 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9301 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9361 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9421 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     9481 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     9541 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     9601 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9661 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     9721 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
     9781 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
     9841 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
     9901 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
     9961 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
    10021 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
    10081 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
    10141 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
    10201 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
    10261 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
    10321 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
    10381 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
    10441 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa
    10501 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
    10561 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
    10621 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
    10681 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg
    10741 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg
    10801 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt
    10861 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
    10921 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
    10981 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
    11041 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
    11101 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
    11161 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
    11221 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
    11281 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
    11341 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
    11401 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
    11461 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
    11521 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
    11581 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    11641 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    11701 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctagtggt
    11761 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    11821 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
    11881 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
    11941 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
    12001 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
    12061 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
    12121 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
    12181 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
    12241 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
    12301 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    12361 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    12421 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    12481 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
    12541 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
    12601 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
    12661 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
    12721 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
    12781 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
    12841 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
    12901 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    12961 caaaacaaaa gtaagaccac cgcacagcaa gcggccggtg atcttcagac ctggacgata
    13021 tatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
    13081 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
    13141 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
    13201 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
    13261 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
    13321 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
    13381 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
    13441 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
    13501 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
    13561 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
    13621 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
    13681 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
    13741 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
    13801 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
    13861 cggtcg
//
