LOCUS       V030054                13275 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030054
VERSION     V030054
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2731
                     /label="G2E3(NM_017769)"
                     /note="G2E3(NM_017769)"
                     /gene="G2E3"
     CDS             2732..2758
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2804..3145
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3271..3770
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3791..4498
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4499..4555
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4556..5155
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5166..5754
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5817..6050
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6075..6093)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6882..6986
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6987..7847
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8018..8606
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8852..9181
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10315..10380
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10510..10530
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10955..11089
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11258..11891
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11938..12063
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12555..12788
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12973..13017
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctcatttc agcgcagtga
      601 actccaagcc accatgaatg aaagtaaacc tggtgactca cagaaccttg cttgtgtttt
      661 ctgtcgaaaa catgatgact gtcctaataa atacggagaa aagaaaacta aggagaaatg
      721 gaatctcact gtacattact actgtttgtt gatgtcaagt ggaatttggc agagaggcaa
      781 agaagaagaa ggagtttatg gttttctaat agaagatatc aggaaggaag tgaatagagc
      841 ttctaaactg aaatgctgtg tttgcaagaa aaatggtgct tcaattggat gtgttgcacc
      901 ccgatgtaaa cgaagttatc atttcccatg tggacttcag agagaatgta ttttccagtt
      961 tactggcaat tttgcgtcat tttgttggga ccatcgacct gttcaaataa ttacatctaa
     1021 taattataga gagtccttac catgcaccat ttgcttggaa tttattgagc ctattccaag
     1081 ttataacata ttacgaagtc cttgttgtaa gaacgcttgg tttcatagag actgtttaca
     1141 ggttcaagca ataaatgcgg gagtgttttt ctttaggtgt acaatatgca ataatagtga
     1201 catctttcag aaagagatgt tgagaatggg aattcatatt cctgaaaaag atgcttcctg
     1261 ggaattagag gaaaacgctt atcaagagct tctgcagcac tatgagcgtt gtgatgttcg
     1321 aagatgtcgt tgcaaagaag ggcgagacta taatgcacct gatagcaaat gggaaataaa
     1381 gcgctgtcag tgttgtggtt ccagtggcac acatttagcc tgctcctcat tacggtcatg
     1441 ggagcaaaat tgggagtgtt tggaatgtag gggtattatc tacaattcag gagagttcca
     1501 aaaagccaaa aaacatgtat tacccaattc taataatgtg gggattacag attgtttgtt
     1561 ggaagagtca tcacctaaat tacccagaca gtcacctgga tcccagagta aagatctact
     1621 gaggcaaggc agcaaattta gaagaaatgt atcaacacta ttaatagagt taggattcca
     1681 aattaaaaaa aaaactaaaa gattgtatat caacaaagcc aatatctgga atagtgcctt
     1741 agatgcattc agaaatcgaa actttaatcc ttcatatgca attgaagtag catatgttat
     1801 tgaaaatgat aattttggaa gtgagcatcc tggatcaaag caagaatttc tgagtctctt
     1861 aatgcaacat cttgagaact catcattgtt tgaagggtcc ttgtcaaaga acttgtctct
     1921 aaattctcaa gctctgaaag agaatcttta ctatgaagct ggcaaaatgc ttgccatttc
     1981 tttagttcac ggtggtcctt cacctggttt cttttctaaa accttgttta actgccttgt
     2041 ttatggacca gaaaataccc agccaatttt agatgatgtt tcagactttg atgtggcaca
     2101 gattataatc aggataaata ctgcaacaac tgtagctgac ttaaagtcaa taataaatga
     2161 atgctataac taccttgagt taattggatg tctcagactt ataacgacat taagtgataa
     2221 atatatgtta gtaaaagaca tacttggcta ccatgtaatt cagagagtcc acacaccctt
     2281 tgaaagtttt aagcagggtc tgaaaaccct tggtgttttg gagaaaattc aggcttatcc
     2341 agaagcattt tgtagcatcc tgtgtcataa acctgagagt ctttctgcaa aaatccttag
     2401 tgagcttttt acagtacaca cattacctga tgtgaaagct ttggggtttt ggaacagtta
     2461 cttacaggct gttgaagatg gtaaatctac aacaacaatg gaagacattc ttatttttgc
     2521 aactggttgc agttccattc ctccagctgg atttaaaccc actccttcaa ttgagtgtct
     2581 gcatgtggat tttcctgttg gaaacaagtg taataactgt ttagcaattc ccatcaccaa
     2641 tacatataaa gagtttcaag aaaatatgga cttcaccata agaaacactc taagactaga
     2701 aaaggaagaa agttctcatt acattggaca ttacccctac gacgtgcctg actacgccgg
     2761 cggcggtggt tcaggcggtg gcggttccgg tggtggggga tcagtccaac tggtggagtc
     2821 tggtggcgct ttggtgcagc caggtggctc tctgcgtttg tcctgtgccg cttctggctt
     2881 cccagtgaac cgctattcca tgcgctggta tcgccaggct ccaggcaaag agcgtgagtg
     2941 ggtagccggt atgtccagcg cgggtgatcg tagctcctat gaagactccg tgaagggccg
     3001 tttcaccatc agccgtgacg atgcccgtaa cacggtgtat ctgcaaatga acagcttgaa
     3061 acctgaagat acggccgtgt attactgtaa tgtgaacgtg ggcttcgagt attggggcca
     3121 aggcacccag gtcaccgtct ccagctaaga tccttgactt gcggccgcaa ctcccacctg
     3181 caacatgcgt gactgactga ggccgcgact ctagagtcga cctgcatcta ggcgccggaa
     3241 ttagatctct cgaggttaac gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     3301 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     3361 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     3421 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     3481 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     3541 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     3601 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     3661 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     3721 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
     3781 caagcttacc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     3841 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     3901 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     3961 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     4021 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     4081 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     4141 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     4201 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     4261 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     4321 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     4381 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     4441 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggc
     4501 aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg gaccgatgac
     4561 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     4621 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     4681 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     4741 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     4801 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     4861 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     4921 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     4981 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     5041 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     5101 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgatctta
     5161 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     5221 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5281 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5341 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     5401 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5461 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     5521 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     5581 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     5641 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     5701 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     5761 accgtcgacc aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     5821 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     5881 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     5941 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6001 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa
     6061 ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg
     6121 tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc
     6181 ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga
     6241 cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc
     6301 agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc
     6361 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
     6421 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
     6481 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
     6541 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
     6601 atggtgcact ctcagtacaa tctagctctg atgccgcata gttaagccag ccccgacacc
     6661 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     6721 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     6781 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     6841 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6901 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6961 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     7021 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     7081 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     7141 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     7201 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     7261 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     7321 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7381 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7441 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7501 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     7561 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     7621 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7681 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     7741 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7801 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7861 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7921 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7981 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8041 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8101 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8161 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8221 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8281 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8341 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8401 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8461 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8521 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8581 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8641 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8701 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8761 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     8821 gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     8881 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     8941 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     9001 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     9061 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     9121 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     9181 aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt
     9241 cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc
     9301 cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata
     9361 aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga
     9421 tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg
     9481 ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca
     9541 tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct
     9601 gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg
     9661 tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc
     9721 aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg
     9781 gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc
     9841 ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt
     9901 ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt
     9961 tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg
    10021 cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga
    10081 ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg
    10141 cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg
    10201 gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac
    10261 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
    10321 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
    10381 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
    10441 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
    10501 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
    10561 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
    10621 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
    10681 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
    10741 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
    10801 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
    10861 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
    10921 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
    10981 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    11041 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
    11101 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
    11161 cctagtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    11221 attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa
    11281 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
    11341 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
    11401 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
    11461 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
    11521 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
    11581 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
    11641 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
    11701 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
    11761 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    11821 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    11881 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
    11941 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
    12001 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
    12061 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
    12121 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
    12181 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
    12241 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
    12301 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
    12361 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggtga tcttcagacc
    12421 tggacgatat atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
    12481 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
    12541 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
    12601 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
    12661 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
    12721 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
    12781 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
    12841 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
    12901 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
    12961 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
    13021 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
    13081 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
    13141 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
    13201 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
    13261 ggatctcgac ggtcg
//
