LOCUS       V033403                12207 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033403
VERSION     V033403
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..1663
                     /label="DTX3(NM_001286245)"
                     /note="DTX3(NM_001286245)"
                     /gene="DTX3"
     CDS             1664..1690
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1736..2077
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2203..2702
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             2723..3430
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3431..3487
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3488..4087
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4098..4686
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             4749..4982
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5007..5025)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        5814..5918
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             5919..6779
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      6950..7538
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        7784..8113
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9247..9312
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9442..9462
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    9887..10021
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10190..10823
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    10870..10995
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11487..11720
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             11905..11949
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctgattga atcgcagtga
      601 actccaagcc accatgccaa ttctaagctc ttcaggatca aaaatggcag cctgtggagg
      661 cacctgcaag aacaaagtga ctgtgtccaa gcccgtgtgg gacttcctga gcaaagagac
      721 cccagcccgg ctggcccggc ttcgggagga gcaccgtgtg tccatcctca tagatggcga
      781 gacttctgac atctatgttc tccagctttc cccacagggt cctcccccgg cccctccaaa
      841 tgggctctac ctagcccgga aggctctcaa ggggctgcta aaagaggcag agaaagagct
      901 gaagaaagct cagaggcagg gggagctgat gggctgcctg gctctggggg gtggagggga
      961 gcaccctgag atgcaccgcg caggcccacc ccctctccga gcagccccac ttctgccccc
     1021 aggagctcgg gggctccccc ctcctcctcc ccccctgccc ccacctcttc ctcctcgcct
     1081 tcgggaggag gcagaagagc aggagagcac ctgccccatc tgtctggggg agatccagaa
     1141 tgccaagaca ttggagaagt gccggcattc attctgcgag ggctgcatca cccgggctct
     1201 gcaggtgaaa aaggcctgcc ccatgtgcgg ccgcttctat gggcagctgg tgggcaacca
     1261 gccccagaat gggcggatgc tggtctctaa ggacgccacc ctcctactgc ccagctatga
     1321 gaagtacggc accattgtca tccagtacgt cttcccgccc ggtgtccagg gggctgaaca
     1381 cccaaaccca ggagttcggt atcctggcac cacacgggtg gcctacctcc cggactgccc
     1441 tgagggcaac aaggtgctga ccctgttccg caaggcgttt gaccagcgtc tcaccttcac
     1501 tatcggcacg tccatgacca cagggagacc gaatgtcatc acctggaacg acatccacca
     1561 caagaccagc tgcacagggg gaccccagct gtttgggtac ccagacccca cctacctgac
     1621 ccgggtgcaa gaggagctga gagcgaaggg tatcacagat gactacccct acgacgtgcc
     1681 tgactacgcc ggcggcggtg gttcaggcgg tggcggttcc ggtggtgggg gatcagtcca
     1741 actggtggag tctggtggcg ctttggtgca gccaggtggc tctctgcgtt tgtcctgtgc
     1801 cgcttctggc ttcccagtga accgctattc catgcgctgg tatcgccagg ctccaggcaa
     1861 agagcgtgag tgggtagccg gtatgtccag cgcgggtgat cgtagctcct atgaagactc
     1921 cgtgaagggc cgtttcacca tcagccgtga cgatgcccgt aacacggtgt atctgcaaat
     1981 gaacagcttg aaacctgaag atacggccgt gtattactgt aatgtgaacg tgggcttcga
     2041 gtattggggc caaggcaccc aggtcaccgt ctccagctaa gatccttgac ttgcggccgc
     2101 aactcccacc tgcaacatgc gtgactgact gaggccgcga ctctagagtc gacctgcatc
     2161 taggcgccgg aattagatct ctcgaggtta acgaattcta ccgggtaggg gaggcgcttt
     2221 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     2281 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     2341 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     2401 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     2461 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     2521 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     2581 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     2641 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     2701 cgacctgcag cccaagctta ccatggtgag caagggcgag gaggataaca tggccatcat
     2761 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     2821 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     2881 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     2941 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     3001 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     3061 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     3121 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     3181 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     3241 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     3301 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     3361 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     3421 gctgtacaag gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc
     3481 tggaccgatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     3541 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     3601 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     3661 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     3721 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     3781 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     3841 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     3901 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     3961 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     4021 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     4081 tgcctgatct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4141 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4201 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     4261 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4321 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     4381 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     4441 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     4501 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     4561 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     4621 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     4681 ccccgcatcg ataccgtcga ccaaggcagc tgtagatctt agccactttt taaaagaaaa
     4741 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     4801 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     4861 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     4921 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     4981 cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata
     5041 cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt
     5101 gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc
     5161 ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc
     5221 agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc
     5281 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     5341 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     5401 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
     5461 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     5521 ggagagcgtc gaatggtgca ctctcagtac aatctagctc tgatgccgca tagttaagcc
     5581 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5641 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5701 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5761 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5821 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5881 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5941 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6001 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6061 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6121 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6181 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6241 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6301 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6361 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6421 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6481 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6541 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6601 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6661 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6721 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6781 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6841 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6901 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6961 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7021 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7081 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7141 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7201 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7261 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7321 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7381 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7441 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7501 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7561 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7621 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7681 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7741 ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg
     7801 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7861 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7921 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     7981 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8041 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8101 aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac
     8161 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
     8221 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
     8281 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
     8341 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
     8401 aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact
     8461 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
     8521 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
     8581 ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt
     8641 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc
     8701 gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc
     8761 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg
     8821 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
     8881 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
     8941 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact
     9001 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc
     9061 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg
     9121 atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg
     9181 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
     9241 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
     9301 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
     9361 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
     9421 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
     9481 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
     9541 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
     9601 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
     9661 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
     9721 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
     9781 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     9841 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     9901 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9961 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    10021 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
    10081 cactgccaat tacctagtgg tttcatttac tctaaacctg tgattcctct gaattatttt
    10141 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta
    10201 attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
    10261 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
    10321 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
    10381 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
    10441 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
    10501 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
    10561 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
    10621 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
    10681 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    10741 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    10801 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
    10861 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
    10921 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
    10981 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
    11041 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
    11101 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
    11161 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
    11221 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
    11281 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggt
    11341 gatcttcaga cctggacgat atatatgagg gacaattgga gaagtgaatt atataaatat
    11401 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
    11461 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
    11521 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
    11581 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
    11641 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
    11701 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
    11761 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
    11821 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
    11881 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
    11941 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
    12001 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
    12061 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
    12121 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
    12181 cgattagtga acggatctcg acggtcg
//
