LOCUS       V030633                13497 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030633
VERSION     V030633
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2953
                     /label="CUL5(NM_003478)"
                     /note="CUL5(NM_003478)"
                     /gene="CUL5"
     CDS             2954..2980
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3026..3367
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3493..3992
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4013..4720
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4721..4777
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4778..5377
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5388..5976
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6039..6272
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6297..6315)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7104..7208
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7209..8069
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8240..8828
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9074..9403
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10537..10602
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10732..10752
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11177..11311
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11480..12113
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12160..12285
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12777..13010
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13195..13239
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctgaccca tcggcagtga
      601 actccaagcc accatggcga cgtctaatct gttaaagaat aaaggttctc ttcagtttga
      661 agacaaatgg gattttatgc gcccgattgt tttgaagctt ttacgccagg aatctgttac
      721 aaaacagcag tggtttgatc tgttttcgga tgtgcatgca gtctgtcttt gggatgataa
      781 aggcccagca aaaattcatc aggctttaaa agaagatatt cttgagttta ttaagcaagc
      841 acaggcacga gtactgagcc atcaagatga tacggctttg ctaaaagcat atattgttga
      901 atggcgaaag ttctttacac aatgtgatat tttaccaaaa cctttttgtc aactagagat
      961 tactttaatg ggtaaacagg gcagcaataa aaaatcaaat gtggaagaca gtattgttcg
     1021 aaagcttatg cttgatacat ggaatgagtc aatcttttca aacataaaaa acagactcca
     1081 agatagtgca atgaagctgg tacatgctga gagattggga gaagcttttg attctcagct
     1141 ggttattgga gtaagagaat cctatgttaa cctttgttct aatcctgagg ataaacttca
     1201 aatttatagg gacaattttg agaaggcata cttggattca acagagagat tttatagaac
     1261 acaagcaccc tcgtatttac aacaaaatgg tgtacagaat tatatgaaat atgcagatgc
     1321 taaattaaaa gaagaagaaa aacgagcact acgttattta gaaacaagac gagaatgtaa
     1381 ctccgttgaa gcactcatgg aatgctgtgt aaatgccctg gtgacatcat ttaaagagac
     1441 tatcttagct gagtgccaag gcatgatcaa gagaaatgaa actgaaaaat tacatttaat
     1501 gttttcattg atggacaaag ttcctaatgg tatagagcca atgttgaaag acttggagga
     1561 acatatcatt agtgctggcc tggcagatat ggtagcagct gctgaaacta ttactactga
     1621 ctctgagaaa tacgttgagc agttacttac actatttaat agatttagta aactcgtcaa
     1681 agaagctttt caagatgatc cacgatttct tactgcaaga gataaggcgt ataaagcagt
     1741 tgttaatgat gctaccatat ttaaacttga attacctttg aagcagaagg gggtgggatt
     1801 aaaaactcag cctgaatcaa aatgccctga gctgcttgcc aattactgtg acatgttgct
     1861 aagaaaaaca ccattaagca aaaaactaac ctctgaagag attgaagcaa agcttaaaga
     1921 agtgctcttg gtacttaagt atgtacagaa caaagatgtt tttatgaggt atcataaagc
     1981 tcatttgaca cgacgtctta tattagacat ctctgccgat agtgaaattg aagaaaacat
     2041 ggtagagtgg ctaagagaag ttggtatgcc agcggattat gtaaacaagc ttgctagaat
     2101 gtttcaggac ataaaagtat ctgaagattt gaaccaagct tttaaggaaa tgcacaaaaa
     2161 taataaattg gcattaccag ctgattcagt taatataaaa attctgaatg ctggcgcctg
     2221 gtcaagaagt tctgagaaag tctttgtctc acttcctact gaactggagg acttgatacc
     2281 ggaagtagaa gaattctaca aaaaaaatca tagtggtaga aaattacatt ggcatcatct
     2341 catgtcaaat ggaattataa catttaagaa tgaagttggt caatatgatt tggaggtaac
     2401 cacgtttcag ctcgctgtat tgtttgcatg gaaccaaaga cccagagaga aaatcagctt
     2461 tgaaaatctt aagcttgcaa ctgaactccc tgatgctgaa cttaggagga ctttatggtc
     2521 tttagtagct ttcccaaaac tcaaacggca agttttgttg tatgaacctc aagtcaactc
     2581 acccaaagac tttacagaag gtaccctctt ctcagtgaac caggagttca gtttaataaa
     2641 aaatgcaaag gttcagaaaa ggggtaaaat caacttgatt ggacgtttgc agctcactac
     2701 agaaaggatg agagaagaag agaatgaagg aatagttcaa ctacgaatac taagaaccca
     2761 ggaagctatc atacaaataa tgaaaatgag aaagaaaatt agtaatgctc agctgcagac
     2821 tgaattagta gaaattttga aaaacatgtt cttgccacaa aagaaaatga taaaagagca
     2881 aatagagtgg ctaatagagc acaaatacat cagaagagat gaatctgata tcaacacttt
     2941 catatatatg gcatacccct acgacgtgcc tgactacgcc ggcggcggtg gttcaggcgg
     3001 tggcggttcc ggtggtgggg gatcagtcca actggtggag tctggtggcg ctttggtgca
     3061 gccaggtggc tctctgcgtt tgtcctgtgc cgcttctggc ttcccagtga accgctattc
     3121 catgcgctgg tatcgccagg ctccaggcaa agagcgtgag tgggtagccg gtatgtccag
     3181 cgcgggtgat cgtagctcct atgaagactc cgtgaagggc cgtttcacca tcagccgtga
     3241 cgatgcccgt aacacggtgt atctgcaaat gaacagcttg aaacctgaag atacggccgt
     3301 gtattactgt aatgtgaacg tgggcttcga gtattggggc caaggcaccc aggtcaccgt
     3361 ctccagctaa gatccttgac ttgcggccgc aactcccacc tgcaacatgc gtgactgact
     3421 gaggccgcga ctctagagtc gacctgcatc taggcgccgg aattagatct ctcgaggtta
     3481 acgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     3541 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     3601 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     3661 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     3721 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     3781 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     3841 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     3901 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     3961 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatggtgag
     4021 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     4081 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     4141 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     4201 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     4261 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     4321 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     4381 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     4441 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     4501 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     4561 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     4621 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     4681 gggccgccac tccaccggcg gcatggacga gctgtacaag gcaacaaact tctctctgct
     4741 gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg accgagtaca agcccacggt
     4801 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     4861 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     4921 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     4981 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     5041 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     5101 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     5161 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     5221 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     5281 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     5341 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgatct tatcgataat caacctctgg
     5401 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5461 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5521 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5581 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5641 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5701 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5761 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     5821 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     5881 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     5941 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga ccaaggcagc
     6001 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6061 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     6121 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6181 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6241 acccttttag tcagtgtgga aaatctctag cagcatctag aattaattcc gtgtattcta
     6301 tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt
     6361 tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta
     6421 tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct
     6481 ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc
     6541 cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
     6601 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
     6661 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
     6721 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
     6781 ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac
     6841 aatctagctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     6901 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     6961 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     7021 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     7081 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     7141 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     7201 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     7261 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     7321 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     7381 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     7441 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7501 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7561 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7621 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7681 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7741 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7801 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7861 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7921 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7981 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     8041 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     8101 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     8161 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8221 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8281 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8341 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     8401 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8461 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8521 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8581 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8641 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8701 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8761 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8821 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8881 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8941 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     9001 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     9061 gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     9121 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     9181 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     9241 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     9301 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     9361 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac
     9421 aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
     9481 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg
     9541 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
     9601 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
     9661 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
     9721 tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat
     9781 ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga
     9841 tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat
     9901 tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc
     9961 gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga
    10021 tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact
    10081 gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg
    10141 aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac
    10201 atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt
    10261 cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga
    10321 tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg
    10381 atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact
    10441 ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa
    10501 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
    10561 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
    10621 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
    10681 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
    10741 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
    10801 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
    10861 gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac
    10921 agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt
    10981 aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag
    11041 gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga
    11101 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
    11161 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    11221 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    11281 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
    11341 tcccaaactt cccaccccat accctattac cactgccaat tacctagtgg tttcatttac
    11401 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
    11461 gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt
    11521 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
    11581 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
    11641 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
    11701 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
    11761 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
    11821 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
    11881 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
    11941 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    12001 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    12061 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
    12121 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
    12181 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
    12241 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
    12301 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
    12361 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
    12421 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
    12481 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
    12541 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
    12601 agtaagacca ccgcacagca agcggccggt gatcttcaga cctggacgat atatatgagg
    12661 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
    12721 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
    12781 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
    12841 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
    12901 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
    12961 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
    13021 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
    13081 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
    13141 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
    13201 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
    13261 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
    13321 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
    13381 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
    13441 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtcg
//
