LOCUS       V030103                12972 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030103
VERSION     V030103
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2428
                     /label="BTRC(NM_033637)"
                     /note="BTRC(NM_033637)"
                     /gene="BTRC"
     CDS             2429..2455
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2501..2842
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2968..3467
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3488..4195
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4196..4252
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4253..4852
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4863..5451
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5514..5747
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5772..5790)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6579..6683
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6684..7544
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7715..8303
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8549..8878
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10012..10077
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10207..10227
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10652..10786
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10955..11588
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11635..11760
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12252..12485
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12670..12714
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctaaaatt cctgcagtga
      601 actccaagcc accatggacc cggccgaggc ggtgctgcaa gagaaggcac tcaagtttat
      661 gtgctctatg cccaggtctc tgtggctggg ctgctccagc ctggcggaca gcatgccttc
      721 gctgcgatgc ctgtataacc cagggactgg cgcactcaca gctttccaga attcctcaga
      781 gagagaagac tgtaataatg gcgaaccccc taggaagata ataccagaga agaattcact
      841 tagacagaca tacaacagct gtgccagact ctgcttaaac caagaaacag tatgtttagc
      901 aagcactgct atgaagactg agaattgtgt ggccaaaaca aaacttgcca atggcacttc
      961 cagtatgatt gtgcccaagc aacggaaact ctcagcaagc tatgaaaagg aaaaggaact
     1021 gtgtgtcaaa tactttgagc agtggtcaga gtcagatcaa gtggaatttg tggaacatct
     1081 tatatcccaa atgtgtcatt accaacatgg gcacataaac tcgtatctta aacctatgtt
     1141 gcagagagat ttcataactg ctctgccagc tcggggattg gatcatattg ctgagaacat
     1201 tctgtcatac ctggatgcca aatcactatg tgctgctgaa cttgtgtgca aggaatggta
     1261 ccgagtgacc tctgatggca tgctgtggaa gaagcttatc gagagaatgg tcaggacaga
     1321 ttctctgtgg agaggcctgg cagaacgaag aggatgggga cagtatttat tcaaaaacaa
     1381 acctcctgac gggaatgctc ctcccaactc tttttataga gcactttatc ctaaaattat
     1441 acaagacatt gagacaatag aatctaattg gagatgtgga agacatagtt tacagagaat
     1501 tcactgccga agtgaaacaa gcaaaggagt ttactgttta cagtatgatg atcagaaaat
     1561 agtaagcggc cttcgagaca acacaatcaa gatctgggat aaaaacacat tggaatgcaa
     1621 gcgaattctc acaggccata caggttcagt cctctgtctc cagtatgatg agagagtgat
     1681 cataacagga tcatcggatt ccacggtcag agtgtgggat gtaaatacag gtgaaatgct
     1741 aaacacgttg attcaccatt gtgaagcagt tctgcacttg cgtttcaata atggcatgat
     1801 ggtgacctgc tccaaagatc gttccattgc tgtatgggat atggcctccc caactgacat
     1861 taccctccgg agggtgctgg tcggacaccg agctgctgtc aatgttgtag actttgatga
     1921 caagtacatt gtttctgcat ctggggatag aactataaag gtatggaaca caagtacttg
     1981 tgaatttgta aggaccttaa atggacacaa acgaggcatt gcctgtttgc agtacaggga
     2041 caggctggta gtgagtggct catctgacaa cactatcaga ttatgggaca tagaatgtgg
     2101 tgcatgttta cgagtgttag aaggccatga ggaattggtg cgttgtattc gatttgataa
     2161 caagaggata gtcagtgggg cctatgatgg aaaaattaaa gtgtgggatc ttgtggctgc
     2221 tttggacccc cgtgctcctg cagggacact ctgtctacgg acccttgtgg agcattccgg
     2281 aagagttttt cgactacagt ttgatgaatt ccagattgtc agtagttcac atgatgacac
     2341 aatcctcatc tgggacttcc taaatgatcc agctgcccaa gctgaacccc cccgttcccc
     2401 ttctcgaaca tacacctaca tctccagata cccctacgac gtgcctgact acgccggcgg
     2461 cggtggttca ggcggtggcg gttccggtgg tgggggatca gtccaactgg tggagtctgg
     2521 tggcgctttg gtgcagccag gtggctctct gcgtttgtcc tgtgccgctt ctggcttccc
     2581 agtgaaccgc tattccatgc gctggtatcg ccaggctcca ggcaaagagc gtgagtgggt
     2641 agccggtatg tccagcgcgg gtgatcgtag ctcctatgaa gactccgtga agggccgttt
     2701 caccatcagc cgtgacgatg cccgtaacac ggtgtatctg caaatgaaca gcttgaaacc
     2761 tgaagatacg gccgtgtatt actgtaatgt gaacgtgggc ttcgagtatt ggggccaagg
     2821 cacccaggtc accgtctcca gctaagatcc ttgacttgcg gccgcaactc ccacctgcaa
     2881 catgcgtgac tgactgaggc cgcgactcta gagtcgacct gcatctaggc gccggaatta
     2941 gatctctcga ggttaacgaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     3001 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     3061 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     3121 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     3181 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     3241 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     3301 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     3361 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     3421 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa
     3481 gcttaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     3541 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     3601 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     3661 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     3721 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     3781 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     3841 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     3901 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     3961 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     4021 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     4081 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     4141 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaaggcaac
     4201 aaacttctct ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatgaccga
     4261 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     4321 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     4381 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     4441 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     4501 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     4561 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     4621 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     4681 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     4741 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     4801 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gatcttatcg
     4861 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     4921 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4981 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     5041 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     5101 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     5161 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     5221 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     5281 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     5341 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5401 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     5461 gtcgaccaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     5521 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     5581 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     5641 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     5701 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta
     5761 attccgtgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat
     5821 taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta
     5881 ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga
     5941 accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc
     6001 agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
     6061 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
     6121 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
     6181 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
     6241 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg
     6301 gtgcactctc agtacaatct agctctgatg ccgcatagtt aagccagccc cgacacccgc
     6361 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     6421 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     6481 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     6541 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6601 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6661 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6721 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6781 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6841 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6901 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6961 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     7021 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7081 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7141 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7201 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7261 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7321 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7381 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7441 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7501 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7561 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7621 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7681 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7741 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7801 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7861 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7921 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7981 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     8041 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8101 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8161 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8221 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8281 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8341 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8401 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8461 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     8521 ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     8581 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     8641 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8701 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     8761 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     8821 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     8881 gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag
     8941 ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag
     9001 atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac
     9061 aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc
     9121 atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg
     9181 taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
     9241 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
     9301 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
     9361 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
     9421 accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat
     9481 tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
     9541 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc
     9601 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
     9661 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
     9721 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
     9781 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
     9841 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
     9901 accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc
     9961 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
    10021 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
    10081 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
    10141 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
    10201 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
    10261 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
    10321 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
    10381 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
    10441 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
    10501 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca
    10561 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
    10621 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
    10681 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    10741 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
    10801 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
    10861 agtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
    10921 gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga
    10981 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
    11041 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
    11101 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
    11161 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
    11221 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
    11281 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
    11341 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
    11401 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
    11461 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
    11521 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
    11581 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
    11641 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
    11701 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
    11761 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
    11821 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
    11881 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
    11941 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
    12001 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
    12061 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggtgatct tcagacctgg
    12121 acgatatata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
    12181 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
    12241 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
    12301 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
    12361 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
    12421 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
    12481 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
    12541 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
    12601 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
    12661 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
    12721 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
    12781 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
    12841 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
    12901 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
    12961 tctcgacggt cg
//
