LOCUS       V033270                12771 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033270
VERSION     V033270
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2227
                     /label="BTBD6(NM_001387567)"
                     /note="BTBD6(NM_001387567)"
                     /gene="BTBD6"
     CDS             2228..2254
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2300..2641
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2767..3266
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3287..3994
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3995..4051
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4052..4651
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4662..5250
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5313..5546
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5571..5589)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6378..6482
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6483..7343
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7514..8102
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8348..8677
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9811..9876
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10006..10026
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10451..10585
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10754..11387
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11434..11559
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12051..12284
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12469..12513
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctattcct gccgcagtga
      601 actccaagcc accatgctgc tgcccctagc ctgcctgcac ggccgcgtcg ctcagtgcct
      661 gacctccttg cttttgcttg cagagccgct cccgaggccc cggcgcggcg cgagggcgcg
      721 gggcgcggcg tccacaggcg ccgaggctgc ccccgccgcc ccgcccgcga agatggcggc
      781 ggaactctac gctcccgcca gcgccgcggc cgcggaccta gccaacagca acgccggcgc
      841 cgccgtgggc aggaaggccg ggccgcgcag cccgcccagc gcccccgcgc cagcgccgcc
      901 gccgcccgcg cccgcgccgc ccacactcgg caacaaccac caggagagcc ccggctggcg
      961 gtgctgccgc cccacgctgc gcgagaggaa cgcgctcatg ttcaacaacg agctcatggc
     1021 cgacgtgcac ttcgtcgtgg ggcccccggg ggcgaccagg acggtgcccg cccacaagta
     1081 cgtcttggct gtcggcagct ccgtcttcta tgccatgttc tacggagacc tggcggaagt
     1141 caaatctgaa attcacattc cagacgtgga gcccgcagcc tttctgatcc tcttaaagta
     1201 catgtacagt gatgagatcg atctggaagc cgacacggtg ctggccactc tgtacgctgc
     1261 taagaagtac atcgtcccag cattggcaaa agcctgtgtc aactttctgg agacaagttt
     1321 ggaagccaag aacgcctgcg tcctgctgtc ccagagccgg ctgtttgagg agcccgagct
     1381 gacgcagcgc tgctgggagg tcattgacgc acaggccgag atggccctac ggtccgaagg
     1441 cttctgtgag atagaccggc agacgctgga gatcattgtc actcgggagg ccctcaacac
     1501 caaagaggcg gtggtcttcg aggccgtcct gaactgggcc gaggcggagt gcaagaggca
     1561 ggggctgcca atcaccccac gaaacaagag gcatgttctg gggcgagccc tctatctggt
     1621 ccgaattcca accatgaccc tagaggagtt tgccaacggc gctgcccagt cagacatcct
     1681 gactctggag gagacccaca gcatcttcct gtggtacacg gccaccaaca agccccgcct
     1741 ggactttccc ctgaccaaga ggaagggcct cgccccgcag aggtgccacc gattccagtc
     1801 ttctgcctac cgcagcaacc agtggcggta ccgcgggcgc tgcgacagca tccagtttgc
     1861 agtggacaga agggtattta ttgcagggct gggcctgtat ggctccagct ctgggaaggc
     1921 tgagtacagc gtgaagattg agctcaagcg gctcggggtg gttctggctc agaacttgac
     1981 caagttcatg tcagacggat ccagtaacac cttcccggtc tggtttgaac acccggtcca
     2041 ggttgaacaa gacaccttct acacggccag tgccgtcctg gacggcagcg aactcagcta
     2101 ctttgggcag gaggggatga cggaagtgca gtgtggaaag gtggccttcc agttccagtg
     2161 ctcctcggac agcaccaacg ggactggggt ccagggtggg cagatccctg agctcatttt
     2221 ctatgcctac ccctacgacg tgcctgacta cgccggcggc ggtggttcag gcggtggcgg
     2281 ttccggtggt gggggatcag tccaactggt ggagtctggt ggcgctttgg tgcagccagg
     2341 tggctctctg cgtttgtcct gtgccgcttc tggcttccca gtgaaccgct attccatgcg
     2401 ctggtatcgc caggctccag gcaaagagcg tgagtgggta gccggtatgt ccagcgcggg
     2461 tgatcgtagc tcctatgaag actccgtgaa gggccgtttc accatcagcc gtgacgatgc
     2521 ccgtaacacg gtgtatctgc aaatgaacag cttgaaacct gaagatacgg ccgtgtatta
     2581 ctgtaatgtg aacgtgggct tcgagtattg gggccaaggc acccaggtca ccgtctccag
     2641 ctaagatcct tgacttgcgg ccgcaactcc cacctgcaac atgcgtgact gactgaggcc
     2701 gcgactctag agtcgacctg catctaggcg ccggaattag atctctcgag gttaacgaat
     2761 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     2821 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     2881 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     2941 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     3001 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     3061 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     3121 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     3181 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     3241 cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatgg tgagcaaggg
     3301 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     3361 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     3421 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     3481 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     3541 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     3601 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     3661 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     3721 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     3781 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     3841 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     3901 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     3961 ccactccacc ggcggcatgg acgagctgta caaggcaaca aacttctctc tgctgaaaca
     4021 agccggagat gtcgaagaga atcctggacc gatgaccgag tacaagccca cggtgcgcct
     4081 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     4141 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     4201 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     4261 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     4321 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     4381 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     4441 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     4501 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     4561 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     4621 ctggtgcatg acccgcaagc ccggtgcctg atcttatcga taatcaacct ctggattaca
     4681 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4741 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4801 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4861 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4921 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4981 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5041 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     5101 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5161 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5221 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgaccaagg cagctgtaga
     5281 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     5341 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5401 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5461 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5521 ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt
     5581 cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac
     5641 gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct
     5701 ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac
     5761 gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc
     5821 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     5881 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     5941 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     6001 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     6061 ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatcta
     6121 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     6181 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     6241 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     6301 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     6361 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6421 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6481 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6541 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6601 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6661 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6721 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6781 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6841 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     6901 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     6961 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7021 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7081 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7141 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7201 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7261 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7321 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7381 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7441 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7501 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7561 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7621 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     7681 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7741 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7801 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7861 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7921 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7981 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8041 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8101 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8161 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8221 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8281 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     8341 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8401 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     8461 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     8521 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8581 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     8641 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt
     8701 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     8761 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     8821 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     8881 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     8941 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc
     9001 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
     9061 ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga
     9121 aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga
     9181 aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt
     9241 tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg
     9301 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg
     9361 cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg
     9421 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg
     9481 aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc
     9541 ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc
     9601 gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg
     9661 aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt
     9721 atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     9781 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     9841 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
     9901 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
     9961 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
    10021 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
    10081 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
    10141 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
    10201 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
    10261 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
    10321 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
    10381 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
    10441 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    10501 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    10561 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
    10621 acttcccacc ccatacccta ttaccactgc caattaccta gtggtttcat ttactctaaa
    10681 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    10741 caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg
    10801 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
    10861 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
    10921 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
    10981 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
    11041 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
    11101 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
    11161 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
    11221 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
    11281 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
    11341 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
    11401 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
    11461 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
    11521 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
    11581 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
    11641 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
    11701 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
    11761 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
    11821 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
    11881 accaccgcac agcaagcggc cggtgatctt cagacctgga cgatatatat gagggacaat
    11941 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
    12001 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
    12061 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
    12121 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
    12181 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
    12241 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
    12301 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
    12361 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
    12421 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
    12481 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
    12541 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
    12601 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
    12661 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
    12721 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggtc g
//
