LOCUS       V033830                12603 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033830
VERSION     V033830
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2059
                     /label="BTBD1(NM_025238)"
                     /note="BTBD1(NM_025238)"
                     /gene="BTBD1"
     CDS             2060..2086
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2132..2473
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2599..3098
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3119..3826
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3827..3883
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3884..4483
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4494..5082
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5145..5378
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5403..5421)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6210..6314
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6315..7175
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7346..7934
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8180..8509
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9643..9708
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9838..9858
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10283..10417
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10586..11219
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11266..11391
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11883..12116
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12301..12345
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctacgaca gtggcagtga
      601 actccaagcc accatggcct cactcgggcc tgccgcagct ggggagcagg cgtcgggggc
      661 tgaggcggag ccgggccccg cggggccgcc gccgccgccc tcaccgtcct ctctggggcc
      721 cctgctcccc ctgcagcggg aacctctcta caactggcag gcgaccaagg cgtcgctgaa
      781 ggagcgcttc gccttcctct tcaactcgga gctgctgagc gatgtgcgct tcgtactggg
      841 caagggtcgc ggcgccgccg ccgctggggg cccgcagcgc atccccgccc accgcttcgt
      901 gctggcggcc ggcagcgccg tctttgacgc catgttcaac ggcggcatgg ccaccacgtc
      961 ggccgagatc gagctgccgg acgtggagcc cgcagccttc ctggcgctgc tgagatttct
     1021 atattcagat gaagttcaaa ttggtccaga aacagttatg accactcttt atactgccaa
     1081 gaaatacgca gtcccagcct tggaagcaca ctgtgtagaa tttctcacca aacatcttag
     1141 ggcagataat gcctttatgt tacttactca ggctcgatta tttgatgaac ctcagcttgc
     1201 tagtctttgt ctagatacaa tagacaaaag cacaatggat gcaataagtg cagaagggtt
     1261 tactgatatt gatatagata cactctgtgc agttttagag agagacacac tcagtattcg
     1321 agaaagtcga ctttttggag ctgttgtacg ctgggcagaa gcagaatgtc agagacaaca
     1381 attacctgtg acttttggga ataaacaaaa agttctagga aaagcacttt ccttaatccg
     1441 gttcccactg atgacaattg aggaatttgc agcaggtcct gctcaatctg gaattttgtc
     1501 agatcgtgaa gtggtaaacc tctttcttca ttttactgtc aaccctaaac cccgagttga
     1561 atacattgac cgaccaagat gctgtctcag gggaaaggaa tgctgcatca atagattcca
     1621 gcaagtagaa agccgctggg gttacagtgg gacgagtgat cgaatcagat tcacagttaa
     1681 tagaaggatc tctatagttg gatttggctt gtatggatct attcatggcc ctacagatta
     1741 tcaagtgaat atacagatca ttgaatatga gaaaaagcaa accctgggac agaatgatac
     1801 cggctttagt tgtgatggga cagctaacac attcagggtc atgttcaagg aacccataga
     1861 gatcctgccc aatgtgtgct acacagcatg tgcaacactc aaaggtccag attcccacta
     1921 tggcacaaaa ggattgaaga aagtagtgca tgagacacct gctgcaagca agactgtttt
     1981 tttctttttt agttcccctg gcaataataa tggcacttca atagaagatg gacaaattcc
     2041 agaaatcata ttttatacat acccctacga cgtgcctgac tacgccggcg gcggtggttc
     2101 aggcggtggc ggttccggtg gtgggggatc agtccaactg gtggagtctg gtggcgcttt
     2161 ggtgcagcca ggtggctctc tgcgtttgtc ctgtgccgct tctggcttcc cagtgaaccg
     2221 ctattccatg cgctggtatc gccaggctcc aggcaaagag cgtgagtggg tagccggtat
     2281 gtccagcgcg ggtgatcgta gctcctatga agactccgtg aagggccgtt tcaccatcag
     2341 ccgtgacgat gcccgtaaca cggtgtatct gcaaatgaac agcttgaaac ctgaagatac
     2401 ggccgtgtat tactgtaatg tgaacgtggg cttcgagtat tggggccaag gcacccaggt
     2461 caccgtctcc agctaagatc cttgacttgc ggccgcaact cccacctgca acatgcgtga
     2521 ctgactgagg ccgcgactct agagtcgacc tgcatctagg cgccggaatt agatctctcg
     2581 aggttaacga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     2641 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     2701 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     2761 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     2821 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     2881 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     2941 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     3001 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     3061 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     3121 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     3181 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     3241 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     3301 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     3361 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     3421 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     3481 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     3541 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     3601 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     3661 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     3721 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     3781 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaaggcaa caaacttctc
     3841 tctgctgaaa caagccggag atgtcgaaga gaatcctgga ccgatgaccg agtacaagcc
     3901 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     3961 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     4021 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     4081 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     4141 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     4201 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     4261 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     4321 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     4381 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     4441 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgatcttatc gataatcaac
     4501 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     4561 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     4621 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     4681 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     4741 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     4801 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     4861 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     4921 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     4981 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     5041 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgaccaa
     5101 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5161 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     5221 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5281 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5341 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt
     5401 attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag
     5461 ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag
     5521 accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga
     5581 gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc
     5641 gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     5701 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     5761 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     5821 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     5881 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct
     5941 cagtacaatc tagctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     6001 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     6061 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     6121 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     6181 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6241 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6301 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6361 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6421 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6481 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6541 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6601 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6661 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6721 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6781 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6841 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6901 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6961 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7021 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7081 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7141 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7201 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7261 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7321 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7381 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7441 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     7501 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7561 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7621 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7681 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7741 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7801 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7861 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7921 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7981 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8041 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8101 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     8161 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     8221 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     8281 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     8341 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     8401 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     8461 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca
     8521 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
     8581 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
     8641 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
     8701 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta
     8761 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
     8821 gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt
     8881 ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga
     8941 aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt
     9001 tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg
     9061 tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc
     9121 gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct
     9181 ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta
     9241 ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct
     9301 ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg
     9361 tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga
     9421 tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga
     9481 tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg
     9541 gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg
     9601 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     9661 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     9721 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
     9781 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
     9841 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
     9901 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
     9961 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
    10021 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
    10081 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
    10141 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
    10201 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
    10261 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
    10321 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    10381 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    10441 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tagtggtttc
    10501 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    10561 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
    10621 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
    10681 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
    10741 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
    10801 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
    10861 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
    10921 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
    10981 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
    11041 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    11101 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    11161 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    11221 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
    11281 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
    11341 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
    11401 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
    11461 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
    11521 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
    11581 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
    11641 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
    11701 aacaaaagta agaccaccgc acagcaagcg gccggtgatc ttcagacctg gacgatatat
    11761 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
    11821 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
    11881 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
    11941 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
    12001 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
    12061 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
    12121 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
    12181 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
    12241 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    12301 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    12361 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    12421 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
    12481 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
    12541 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
    12601 tcg
//
