LOCUS       V033333                12435 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033333
VERSION     V033333
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..1891
                     /label="ASB4(NM_016116)"
                     /note="ASB4(NM_016116)"
                     /gene="ASB4"
     CDS             1892..1918
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1964..2305
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2431..2930
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             2951..3658
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3659..3715
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3716..4315
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4326..4914
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             4977..5210
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5235..5253)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6042..6146
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6147..7007
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7178..7766
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8012..8341
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9475..9540
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9670..9690
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10115..10249
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10418..11051
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11098..11223
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11715..11948
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12133..12177
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttcttgcacg ctggcagtga
      601 actccaagcc accatggacg gcaccactgc ccctgtcact aaatctggag ctgccaagtt
      661 agttaagaga aatttccttg aggcgctaaa gtccaatgac ttcggaaaat tgaaggctat
      721 tttgatccaa aggcaaatag atgtggacac tgtttttgaa gtcgaagatg agaatatggt
      781 tttggcatct tataaacaag gttactggtt gcctagctat aaattgaagt cttcctgggc
      841 cacaggcctc catctctctg tcttgtttgg ccatgtggaa tgtcttctgg tgctactgga
      901 ccacaatgct acaatcaact gtagacccaa tgggaaaacc cctcttcacg tggcttgtga
      961 aatggccaat gtggattgtg ttaagatcct ctgtgatcgt ggggcaaagc tcaattgcta
     1021 ctccttaagt ggacacacag ctttgcactt ttgtacaact ccaagttcca ttctctgtgc
     1081 caagcaattg gtttggagag gggcgaatgt gaacatgaag accaacaacc aagatgagga
     1141 gacgcccttg cacacggctg cccacttcgg cctttcggag ctggtggcct tctacgtgga
     1201 acacggggcc atagtggaca gcgtgaatgc ccacatggag acccccctgg ccatcgccgc
     1261 ctactgggcc ctccgcttta aggagcagga gtacagcacg gagcaccacc tggtctgccg
     1321 catgctgctt gactacaaag ccgaagtcaa tgcccgagat gacgacttta aatctcccct
     1381 ccacaaggca gcctggaact gtgaccacgt gctcatgcac atgatgctgg aagctggcgc
     1441 cgaagccaat ctcatggata tcaacggctg tgctgccatc cagtacgtgc tgaaggtcac
     1501 ctccgtgcgc cctgctgccc agcctgagat ctgctaccag ctcctgttga accatggggc
     1561 tgcccgaata taccctccac agttccataa ggtgatacag gcctgccatt cttgtcctaa
     1621 agcaattgaa gttgtagtca atgcctatga acacatcaga tggaacacaa agtggagaag
     1681 agctatcccc gatgatgact tggagaaata ctgggatttt taccactctc tctttactgt
     1741 gtgctgtaac tctccaagga ctctcatgca cttatcgaga tgtgccatta gaagaacatt
     1801 acacaacaga tgccatagag caattccttt gctttccctc ccattgtcat tgaaaaagta
     1861 cttgctttta gagccagagg gaattattta ttacccctac gacgtgcctg actacgccgg
     1921 cggcggtggt tcaggcggtg gcggttccgg tggtggggga tcagtccaac tggtggagtc
     1981 tggtggcgct ttggtgcagc caggtggctc tctgcgtttg tcctgtgccg cttctggctt
     2041 cccagtgaac cgctattcca tgcgctggta tcgccaggct ccaggcaaag agcgtgagtg
     2101 ggtagccggt atgtccagcg cgggtgatcg tagctcctat gaagactccg tgaagggccg
     2161 tttcaccatc agccgtgacg atgcccgtaa cacggtgtat ctgcaaatga acagcttgaa
     2221 acctgaagat acggccgtgt attactgtaa tgtgaacgtg ggcttcgagt attggggcca
     2281 aggcacccag gtcaccgtct ccagctaaga tccttgactt gcggccgcaa ctcccacctg
     2341 caacatgcgt gactgactga ggccgcgact ctagagtcga cctgcatcta ggcgccggaa
     2401 ttagatctct cgaggttaac gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     2461 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     2521 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     2581 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     2641 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     2701 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     2761 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     2821 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     2881 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
     2941 caagcttacc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     3001 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     3061 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     3121 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     3181 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     3241 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     3301 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     3361 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     3421 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     3481 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     3541 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     3601 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggc
     3661 aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg gaccgatgac
     3721 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     3781 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     3841 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     3901 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     3961 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     4021 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     4081 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     4141 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     4201 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     4261 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgatctta
     4321 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4381 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4441 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4501 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4561 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     4621 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4681 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     4741 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     4801 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     4861 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     4921 accgtcgacc aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     4981 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     5041 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     5101 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     5161 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa
     5221 ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg
     5281 tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc
     5341 ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga
     5401 cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc
     5461 agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc
     5521 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
     5581 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
     5641 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
     5701 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
     5761 atggtgcact ctcagtacaa tctagctctg atgccgcata gttaagccag ccccgacacc
     5821 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     5881 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     5941 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     6001 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6061 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6121 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6181 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6241 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6301 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6361 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6421 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6481 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6541 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6601 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6661 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6721 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6781 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6841 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6901 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6961 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7021 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7081 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7141 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7201 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7261 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7321 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7381 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7441 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7501 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7561 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7621 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7681 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7741 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7801 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7861 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7921 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7981 gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     8041 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     8101 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     8161 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     8221 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     8281 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     8341 aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt
     8401 cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc
     8461 cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata
     8521 aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga
     8581 tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg
     8641 ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca
     8701 tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct
     8761 gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg
     8821 tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc
     8881 aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg
     8941 gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc
     9001 ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt
     9061 ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt
     9121 tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg
     9181 cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga
     9241 ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg
     9301 cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg
     9361 gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac
     9421 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     9481 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     9541 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
     9601 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
     9661 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
     9721 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
     9781 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
     9841 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
     9901 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
     9961 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
    10021 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
    10081 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
    10141 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    10201 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
    10261 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
    10321 cctagtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    10381 attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa
    10441 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
    10501 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
    10561 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
    10621 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
    10681 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
    10741 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
    10801 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
    10861 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
    10921 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    10981 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    11041 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
    11101 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
    11161 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
    11221 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
    11281 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
    11341 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
    11401 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
    11461 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
    11521 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggtga tcttcagacc
    11581 tggacgatat atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
    11641 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
    11701 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
    11761 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
    11821 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
    11881 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
    11941 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
    12001 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
    12061 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
    12121 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
    12181 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
    12241 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
    12301 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
    12361 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
    12421 ggatctcgac ggtcg
//
