LOCUS       V033470                12918 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033470
VERSION     V033470
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2374
                     /label="ASB14(NM_001142733)"
                     /note="ASB14(NM_001142733)"
                     /gene="ASB14"
     CDS             2375..2401
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2447..2788
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2914..3413
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3434..4141
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4142..4198
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4199..4798
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4809..5397
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5460..5693
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5718..5736)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6525..6629
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6630..7490
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7661..8249
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8495..8824
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9958..10023
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10153..10173
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10598..10732
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10901..11534
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11581..11706
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12198..12431
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12616..12660
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctttacat cttgcagtga
      601 actccaagcc accatggata attacaccag cgatgaagac atagatgaag actttgacac
      661 ccagctcatc attcaacaga gtttgcagga tatttataag ccaggaacag cacaacatgc
      721 acctaaggat gagagtttgc attccttttt gagcgctgac tataagaaga tagttgaaac
      781 aatagagaaa ggtaaggaag atgcattgtc acacttaacc aagtaccatt ccgcatttgg
      841 tgaagcagat gagataggct ggattcctct gcataaggct gcagtgcaat taaataggaa
      901 aattttggaa ataaccctaa gcgcttcaga ccccagtctg tgggagcaaa ccactcacaa
      961 tggtgaaacg ccactttttt tggctgtcag cagttgcctc ttagaaaatg ccacttttct
     1021 tcttctcaat ggctgcaatc caaatgctaa gaatttcgaa ggcaattctc ctcttcttgc
     1081 agctgtgctg cgtgactgct atgacatggc tgccttgctg atcaactatg gagcagatgt
     1141 caatctgcgt tgtgccaacg agaggacagc tctccacgaa gcagccaaac tgggcagaga
     1201 ggacatggtg aagcttatgc tggtttctgg ggcacaccct gacccacaga gcacgtatgg
     1261 attcactcct cttgctcttg ctgcccaaag tggacacact gaaatcatgg aaatgttact
     1321 gcggaaagga gctaatgctc atggtcaggc ctctgattct tcttccatct tacttgaagc
     1381 cgcaagtgga ggaaatccag atgctgtggc tctcttgctg gagtatggag ctgatgccaa
     1441 catccctaag aattcaggcc acctgcccat ccatgtggca gctgacaggg gccacttact
     1501 agctctaaag atactgattc cagttacgga tcttgctgcc attaagcaga gtgggatcag
     1561 tccagttcac tgtgcagcag caggagcaca tcctcagtgc ctggaactcc tcatccaggc
     1621 tggatttgat gtgaacttca tgctggatca gagaattaac aaacactacg atgaccacag
     1681 gaagtcagct ttgtattttg ctgtatcaaa cagtgacctc tcttcagtca agctgcttct
     1741 gagtgctgga gctctgccta atcaagaccc ggttaactgc ctccagatag ccctcaggat
     1801 gggcaactat gagctgatca gtctgctgct aaggcatggg gccaatgtca attacttctg
     1861 cagagttaac cctttacatt tcccatcagc actgcaatac actctgaaag atgaagtcat
     1921 gctcaggatg ctgctgaact atgggtatga cacagagcga tgttttgatt gcccacatgg
     1981 agacaaagtc catccttcct atactgttga aggctggaca tctacagtta tcaaagatac
     2041 taagttctgt gaagtaataa ctttgtcatg gctgcaacat ctctctggaa aggttgttcg
     2101 agtgatgctt gattatgttg atcaagttcg gatctgttca aagttgaaag ctgtgctcca
     2161 aaaacagggg atctggtcag aaatacattt tatcttaaca aaccctcgct ccctaaaaca
     2221 tttgtgccgc ctaaagatcc ggaaatgcat gggacggtta catttgcgct gccctgtctt
     2281 catgtcattt cttccattac ccaatcgtct aaaagcatat gtcctttaca aagaatacga
     2341 cctttatgga caaggaattt ttacaggaac ctggtacccc tacgacgtgc ctgactacgc
     2401 cggcggcggt ggttcaggcg gtggcggttc cggtggtggg ggatcagtcc aactggtgga
     2461 gtctggtggc gctttggtgc agccaggtgg ctctctgcgt ttgtcctgtg ccgcttctgg
     2521 cttcccagtg aaccgctatt ccatgcgctg gtatcgccag gctccaggca aagagcgtga
     2581 gtgggtagcc ggtatgtcca gcgcgggtga tcgtagctcc tatgaagact ccgtgaaggg
     2641 ccgtttcacc atcagccgtg acgatgcccg taacacggtg tatctgcaaa tgaacagctt
     2701 gaaacctgaa gatacggccg tgtattactg taatgtgaac gtgggcttcg agtattgggg
     2761 ccaaggcacc caggtcaccg tctccagcta agatccttga cttgcggccg caactcccac
     2821 ctgcaacatg cgtgactgac tgaggccgcg actctagagt cgacctgcat ctaggcgccg
     2881 gaattagatc tctcgaggtt aacgaattct accgggtagg ggaggcgctt ttcccaaggc
     2941 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     3001 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     3061 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     3121 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     3181 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     3241 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     3301 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     3361 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
     3421 gcccaagctt accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     3481 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     3541 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     3601 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     3661 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     3721 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     3781 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     3841 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     3901 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     3961 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     4021 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     4081 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     4141 ggcaacaaac ttctctctgc tgaaacaagc cggagatgtc gaagagaatc ctggaccgat
     4201 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     4261 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     4321 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     4381 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     4441 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     4501 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     4561 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     4621 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     4681 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     4741 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatc
     4801 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4861 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4921 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4981 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     5041 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     5101 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     5161 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     5221 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     5281 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     5341 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     5401 gataccgtcg accaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     5461 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     5521 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     5581 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     5641 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta
     5701 gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt
     5761 atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct
     5821 ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt
     5881 ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca
     5941 atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc
     6001 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     6061 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     6121 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
     6181 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
     6241 cgaatggtgc actctcagta caatctagct ctgatgccgc atagttaagc cagccccgac
     6301 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     6361 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     6421 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     6481 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6541 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6601 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6661 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6721 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6781 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6841 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6901 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6961 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7021 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7081 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7141 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7201 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7261 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7321 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7381 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7441 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7501 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7561 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7621 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7681 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7741 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7801 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7861 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7921 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7981 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8041 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8101 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8161 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8221 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8281 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8341 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8401 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8461 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     8521 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     8581 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8641 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8701 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8761 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     8821 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
     8881 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
     8941 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
     9001 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
     9061 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
     9121 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg
     9181 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
     9241 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
     9301 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
     9361 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
     9421 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
     9481 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
     9541 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
     9601 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
     9661 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca
     9721 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc
     9781 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga
     9841 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct
     9901 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
     9961 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
    10021 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
    10081 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
    10141 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
    10201 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
    10261 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
    10321 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
    10381 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
    10441 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
    10501 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
    10561 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
    10621 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    10681 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
    10741 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
    10801 ttacctagtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    10861 gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc
    10921 caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
    10981 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
    11041 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
    11101 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
    11161 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
    11221 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
    11281 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
    11341 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
    11401 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
    11461 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
    11521 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
    11581 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
    11641 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
    11701 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
    11761 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
    11821 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
    11881 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
    11941 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
    12001 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg tgatcttcag
    12061 acctggacga tatatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
    12121 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
    12181 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
    12241 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
    12301 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
    12361 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
    12421 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
    12481 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
    12541 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
    12601 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
    12661 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
    12721 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
    12781 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
    12841 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
    12901 aacggatctc gacggtcg
//
