LOCUS       V015072                11146 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015072
VERSION     V015072
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11146)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    25..142
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     protein_bind    210..228
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        418..456
                     /label="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        524..542
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             603..629
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             675..1016
                     /label="GFP nanobody"
                     /note="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
     promoter        1142..1641
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             1662..2369
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             2427..3023
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    3037..3625
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             3688..3921
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3946..3964)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        4753..4857
                     /label="AmpR promoter"
     CDS             4858..5715
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5889..6477
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        6723..7052
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     intron          8186..8251
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8381..8401
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    8826..8960
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     LTR             9129..9762
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9809..9934
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    10426..10659
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             10844..10888
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             11037..11078
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctggtacc gtcatcggat
      601 cctaccccta cgacgtgcct gactacgccg gcggcggtgg ttcaggcggt ggcggttccg
      661 gtggtggggg atcagtccaa ctggtggagt ctggtggcgc tttggtgcag ccaggtggct
      721 ctctgcgttt gtcctgtgcc gcttctggct tcccagtgaa ccgctattcc atgcgctggt
      781 atcgccaggc tccaggcaaa gagcgtgagt gggtagccgg tatgtccagc gcgggtgatc
      841 gtagctccta tgaagactcc gtgaagggcc gtttcaccat cagccgtgac gatgcccgta
      901 acacggtgta tctgcaaatg aacagcttga aacctgaaga tacggccgtg tattactgta
      961 atgtgaacgt gggcttcgag tattggggcc aaggcaccca ggtcaccgtc tccagctaag
     1021 atccttgact tgcggccgca actcccacct gcaacatgcg tgactgactg aggccgcgac
     1081 tctagagtcg acctgcatct aggcgccgga attagatctc tcgaggttaa cgaattctac
     1141 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     1201 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     1261 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     1321 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     1381 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     1441 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     1501 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     1561 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     1621 tcctcatctc cgggcctttc gacctgcagc ccaagcttac catggtgagc aagggcgagg
     1681 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     1741 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     1801 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     1861 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     1921 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     1981 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     2041 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     2101 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     2161 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     2221 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     2281 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     2341 ccaccggcgg catggacgag ctgtacaagg caacaaactt ctctctgctg aaacaagccg
     2401 gagatgtcga agagaatcct ggaccgatga ccgagtacaa gcccacggtg cgcctcgcca
     2461 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     2521 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     2581 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     2641 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     2701 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     2761 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     2821 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     2881 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     2941 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     3001 gcatgacccg caagcccggt gcctgatctt atcgataatc aacctctgga ttacaaaatt
     3061 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3121 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3181 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     3241 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3301 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3361 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3421 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     3481 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     3541 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     3601 atctcccttt gggccgcctc cccgcatcga taccgtcgac caaggcagct gtagatctta
     3661 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     3721 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     3781 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     3841 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     3901 cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct
     3961 aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa
     4021 tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc
     4081 gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt
     4141 cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta
     4201 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
     4261 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
     4321 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
     4381 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
     4441 aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctagctct
     4501 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     4561 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     4621 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     4681 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     4741 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4801 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4861 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4921 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     4981 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5041 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5101 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5161 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5221 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5281 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5341 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5401 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5461 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5521 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5581 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5641 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5701 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5761 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5821 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5881 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5941 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6001 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     6061 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6121 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6181 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6241 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6301 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6361 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6421 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6481 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6541 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     6601 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     6661 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga
     6721 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6781 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     6841 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     6901 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6961 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     7021 gaggcttttt tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag
     7081 atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg
     7141 gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt
     7201 ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa
     7261 aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg
     7321 atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg
     7381 cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg
     7441 atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct
     7501 tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt
     7561 atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg
     7621 actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg
     7681 tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg
     7741 ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc
     7801 atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc
     7861 tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat
     7921 ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc
     7981 attgacccac gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt
     8041 accgacgatg atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag
     8101 tgggccccgg atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact
     8161 acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa
     8221 actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga
     8281 gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag
     8341 tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt
     8401 agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag
     8461 taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata
     8521 caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca
     8581 taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc
     8641 tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat
     8701 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg
     8761 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     8821 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8881 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8941 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
     9001 ccaccccata ccctattacc actgccaatt acctagtggt ttcatttact ctaaacctgt
     9061 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
     9121 ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat
     9181 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     9241 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     9301 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     9361 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
     9421 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
     9481 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
     9541 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
     9601 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     9661 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     9721 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     9781 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     9841 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     9901 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     9961 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
    10021 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
    10081 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
    10141 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
    10201 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
    10261 cgcacagcaa gcggccggtg atcttcagac ctggacgata tatatgaggg acaattggag
    10321 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
    10381 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
    10441 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
    10501 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
    10561 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
    10621 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
    10681 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
    10741 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
    10801 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
    10861 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
    10921 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
    10981 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
    11041 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
    11101 agacagagac agatccattc gattagtgaa cggatctcga cggtcg
//
